Depletion is used to reduce the complexity of biological samples such as serum, plasma, or biofluids, which contain high concentrations of albumin and immunoglobulins. These products utilize immunoaffinity techniques to enable the removal of the most abundant proteins, thus enhancing the detection of lower-abundance proteins in both discovery and targeted proteomic analyses. 

  • Efficient—kits remove >95% of albumin, IgG and up to 12 other abundant proteins
  • Fast—spin column formats enable the processing of samples in 5 – 10 minutes
  • Economical—cost-effective spin columns are priced for single use; bulk resins available for customized options
  • Consistent—one-time use helps prevent abundant protein carryover and experimental variability

Choose the right abundant protein depletion product for your mass spec application

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 Pierce Albumin Depletion KitHigh Select HSA/Immunoglobulin Depletion Spin ColumnsHigh Select Top14 Abundant Protein Depletion Spin Columns
 
Pierce Albumin
Depletion Kit
High-Select HSA/Immunoglobulin Depletion Spin Columns
High-Select Top14 Abundant Protein Depletion Spin Columns
Proteins depletedAlbuminAlbumin, IgG, IgG (light chains), IgA, IgM, IgD, IgE14 abundant proteins*
Sample volume capacity10–50 µL10 μL (mini) or
100 μL (midi)		10 μL (mini) or
100 μL (midi)
Processing time20–30 min5–10 min5–10 min
FormatLoose resin, spin columns, buffersPre-filled mini or midi spin columns, bulk resinPre-filled mini or midi spin columns, bulk resin

*Top 14 proteins include Albumin, IgG, IgG (light chains), IgA, IgM, IgD, IgE, α1-Acid glycoprotein, Fibrinogen, Haptoglobin, α1-antitrypsin, α2-macroglobulin, Transferrin, Apolipoprotein A-I

Featured product data

Protein removal achieved using High-Select Abundant Protein Depletion Spin Columns
Figure 1. Protein removal achieved using High-Select Abundant Protein Depletion Spin Columns. (A) Values were determined by targeted MS. (B) The abundant protein depletion percentage was confirmed by ELISA and was in agreement with >99% removal. Immunoglobulins include IgG (KappaXL and Lambda), IgA, IgM, IgD, and IgE.
Abundant protein depletion improves identification of unique proteins
Figure 2. Performance of High-Select Abundant Protein Depletion Spin Columns. Human serum (10–20 μL, Cat. No. 31876) was loaded onto each resin and processed according to protocols. Total protein in the depleted fractions was estimated using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Cat. No. 23225). The total amount of albumin in the depleted fractions was determined using the AssayMax™ Human Albumin ELISA Kit (Assaypro, Cat. No. EA2201-1). FT = flow-through (i.e., depleted sample); E = eluate (i.e., proteins that were bound by the resin, plus stripped affinity antibodies of the column). With the top 14 proteins removed, low-abundance proteins are now visible in each depleted sample lane (FT).
Performance of High-Select Abundant Protein Depletion Spin Columns

Figure 3. Abundant protein depletion improves identification of unique proteins. Protein group identification profiles for normal human plasma samples which were (a) not depleted, or depleted using the HSA/Immunoglobulin (b) and Top14 (c) depletion resins, are shown. All samples were reduced/alkylated and digested with trypsin. Samples were analyzed by LC-MS on an Orbitrap Fusion Tribrid mass spectrometer over a 60 minute gradient using 750 ng of sample per injection (performed in triplicate). Raw files were searched against a human protein database using Thermo Scientific Proteome Discoverer 1.4 software. Non-redundant protein group identification numbers are reported for each sample type.

Depletion results (MS)
Protein removal achieved using High-Select Abundant Protein Depletion Spin Columns
Figure 1. Protein removal achieved using High-Select Abundant Protein Depletion Spin Columns. (A) Values were determined by targeted MS. (B) The abundant protein depletion percentage was confirmed by ELISA and was in agreement with >99% removal. Immunoglobulins include IgG (KappaXL and Lambda), IgA, IgM, IgD, and IgE.
Depletion results (Gel electrophoresis)
Abundant protein depletion improves identification of unique proteins
Figure 2. Performance of High-Select Abundant Protein Depletion Spin Columns. Human serum (10–20 μL, Cat. No. 31876) was loaded onto each resin and processed according to protocols. Total protein in the depleted fractions was estimated using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Cat. No. 23225). The total amount of albumin in the depleted fractions was determined using the AssayMax™ Human Albumin ELISA Kit (Assaypro, Cat. No. EA2201-1). FT = flow-through (i.e., depleted sample); E = eluate (i.e., proteins that were bound by the resin, plus stripped affinity antibodies of the column). With the top 14 proteins removed, low-abundance proteins are now visible in each depleted sample lane (FT).
Improved identification
Performance of High-Select Abundant Protein Depletion Spin Columns

Figure 3. Abundant protein depletion improves identification of unique proteins. Protein group identification profiles for normal human plasma samples which were (a) not depleted, or depleted using the HSA/Immunoglobulin (b) and Top14 (c) depletion resins, are shown. All samples were reduced/alkylated and digested with trypsin. Samples were analyzed by LC-MS on an Orbitrap Fusion Tribrid mass spectrometer over a 60 minute gradient using 750 ng of sample per injection (performed in triplicate). Raw files were searched against a human protein database using Thermo Scientific Proteome Discoverer 1.4 software. Non-redundant protein group identification numbers are reported for each sample type.

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