Cell lysis is the first step when extracting proteins for mass spectrometry applications. Fractionation may be required to enrich for proteins of interest or to study protein localization. The kits featured here are convenient, easy to use, and have been validated for downstream analysis with Thermo Scientific mass spectrometers. They include options for either total protein extraction or isolation of proteins from specific cellular fractions. The Thermo Scientific EasyPep Mini MS Sample Prep Kit provides pre-formulated reagents and a robust method for the preparation of high quality samples that are ready for MS analysis in less than 3 hours.

  • Optimized—extract total protein or specific cellular fractions
  • Effective—enable high protein yields with low cross-contamination for fractionation kits
  • Validated—extract from cultured mammalian cells have been successfully analyzed using Thermo Scientific mass spectrometers
  • Convenient—minimize the need for mechanical cell disruption
     

How to Use video for EasyPep Mini MS Sample Prep Kit

Use this rapid mass spec sample prep kit for protein extraction, digestion and peptide clean-up in 2-4 hours.

Choose the right protein extraction kit for your mass spec workflow

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 Pierce™ Mass Spec Sample Prep Kit for Cultured Cells
 
Thermo Scientific EasyPep Mini MS Sample Prep Kit

Mem-PER Plus Membrane Protein Extraction Kit

Subcellular Protein Fractionation Kit
for Cultured Cells 
Fractions isolatedPrimarily cytosolicIntegral and membrane-associated proteinsNucleus, cytoplasm, membrane, cytoskeletal, chromatin-bound
Amount of sample processed20 samples of 10-100 µg of cell pellet or tissue50 samples of
5 million cells each
50 samples of
2 million cells each
(20 µL packed)
Sample processing time (pre-digestion)<30 min1 hr2–3 hr
Digestion proteases included?YesNoNo
Total sample preparation time1.5-3.5 hr24-48 hr24-48 hr

Featured product data

The EasyPep Mini MS Sample Prep Kit compared to a traditional protocol and another commercially available kit
The EasyPep Mini MS Sample Prep Kit compared to a traditional protocol and another commercially available kit. The EasyPep Mini MS Sample Prep Kit provides a high peptide yield, and comparable peptide and protein identifications in cell lysates with good reproducibility.

 


Representative quantitation

Representative quantitation of protein enrichment of marker proteins (Hsp90 and histone 3) using TMTsixplex reagents in different subcellular fractions relative to whole cell lysate. The red bars show the relative intensity of TMT reagent reporter ions for unique marker proteins. Subcellular fractions were prepared using the Thermo Scientific Subcellular Protein Fractionation Kit for Cultured Cells (Cat. No. 78840).


Identification of peptides by LC-MS/MS from immunoprecipitated Na+/ K+-ATPase transport complex extracted with the Mem-PER Plus Kit (Cat. No. 89842). Samples were processed by in-solution digestion according to the kit protocol, and analyzed on the Orbitrap Fusion mass spectrometer.

ProteinPeptide sequenceXcorr value
Na+/K+-transporting ATPase subunit beta-1VAPPGLTQIPQIQK2.67
VGNVEYFGLGNSPGFPLQYYPYYGK4.81
AYGENIGYSEK2.52
SYEAYVLNIVR3.17
Na+/K+-transporting ATPase subunit alpha-1LSLDELHR2.20
QGAIVAVTGDGVNDSPALK4.03
NIAFFSTNCVEGTAR3.29
SPDFTNENPLETR4.46
TSATWLALSR3.18
AVFQANQENLPILK2.01

仅供科研使用,不可用于诊断目的。