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The SOLiD Library TaqMan Quantitation Kit facilitates the quantitation of amplifiable template in a SOLiD library for use in emulsion PCR (ePCR) on the SOLiD System. Complete with TaqMan qPCR mix custom-designed for SOLiD library adaptors and an adapted SOLiD library control*, the kit is a highly specific solution for determining the optimal amount of starting material needed to most effectively carry out ePCR.
* The SOLiD library control included in the kit is recommended for use only as a qPCR control, and not as a sequence control.
The SOLiD Library TaqMan Quantitation Kit is designed to quantitate libraries accurately regardless of size and can be used on any real-time instrument, using either fast or standard cycling programs. The kit has been validated for the quantitation of SOLiD fragment libraries, SOLiD short and long mate-paired libraries, SOLiD whole transcriptome libraries, SOLiD small RNA libraries, SOLiD Chip-seq libraries, and SOLiD SAGE™ libraries.
The multicolored amplification curves represent six SOLiD Libraries quantitated by the SOLiD Library TaqMan Quantitation Kit (Cat. No. 4449639) on the Applied Biosystems StepOnePlus™ Real-Time PCR System with fast cycling. Using a 5-point serial dilution from 100 pM to 10 fM of the SOLiD Library qPCR Standard, the SOLiD library input can accurately be measured. The gray line represents a no-template control.
仅供科研使用,不可用于诊断目的。