Organism TypeHumanThis is a human cell line with hyperpentaploid chromosome count. The modal chromosome number should be around 128 to 132. The rate of cells with higher ploidies was 1.39%. Fourteen to 16 marker chromosomes were common to most cells. They were: der(1)t(1;?) (p36;?), i(6p), der(10)t(10;?) (q24;?), der (19)t(19;?) (q13;?), der(15)t(15;?) (q26?;?), minute metacentric and eight to ten others. Most of these structurally altered markers had complex interchromosomal exchanges. The der(10) and der(19) could be formed from a balanced translocation, i.e., t(10;19) (q24;q13). These two markers and the minute metacentric were present in three or more copies in most cells. There were six or more copies for N5, N7, N11, N13, N20, N21, and N22 in most cells. The X and N15 had only one copy.
TissuesBrain
PhenotypeAdherent
Primaryno
ApplicationRNAi,Stable Cell Transfection

推荐的转染试剂

  • 质粒 DNA 转染和共转染：Lipofectamine 3000（转染效率为 51-79%，与 Lipofectamine 2000 相比，蛋白表达提高4倍）
  • RNAi 转染 (siRNA/miRNA) 和基因沉默：Lipofectamine RNAiMAX

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