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Volatiles Screening and Quantitation
Blood alcohol screening
生体液体中のアルコールレベルはいくらですか? A fair question, both from forensic and law enforcement stand point.
概要: Blood alcohol content (BAC) analysis is one of the most common preliminary tests in forensic science. However, for purposes of law enforcement, blood alcohol content is used to define the level of intoxication and a rough measure of impairment. It is generally performed via headspace gas chromatography (GC) with a flame ionization detector (FID).
Method: The content of alcohol in blood is most commonly expressed as grams per deciliter (g/dL).
Results: Calibration curves are prepared for methanol, ethanol, isopropanol, and acetone. Method repeatability was tested for most common volatiles on 20 consecutive injections of the same sample.
BAC Analysis workflow
サンプルコレクション
Blood is collected by a phlebotomist in two grey-top tubes that should contain a preservative and anticoagulant (grey top tubes are normally used in medicine for glucose-testing). Typically sodium fluoride is used as preservative. One tube is for the crime lab to test and the other is preserved for the subject’s independent testing purposes. At the crime lab, one tube is opened and the contents are poured into a clean container. A measured amount of blood and a type of alcohol (usually n-propanol) as internal standard are mixed together into a headspace vial, which is then crimp-sealed.
Sample processing
The number of samples run in a day by a forensic lab includes specimen and quality control standards can easily reach several hundreds. Sample preparation often accounts for more than twice the time spent on actual chromatography. Automate and accelerate organic volatiles determinations to increase sample turnaround and lower the cost per analysis. It is paramount that the system will be able to run many unattended analysis and safely classify and store results.
TriPlus 300 Headspace Autosampler
The Thermo Scientific TriPlus 300 Headspace Autosampler: Designed for fast startup, high productivity, and maximum flexibility for any lab analyzing organic volatiles.
- Superior sample integrity and traceability
- End-to-end inertness of the sample path
- Seamless integration into multiple chromatography data systems
- A chromatographer-friendly local user interface further enhances the system's versatility
TriPlus RSH オートサンプラー
高度なロボットを内蔵し、きわめて高い精度やサンプル処理の柔軟性と、週末の無人運転が可能な信頼性を実現します。 Ideal for high throughput lab with quick sample turnaround time.
- Temperature-resistant syringe eliminates the need for a transfer line for direct headspace injection
- Overlapping sample incubation capability allows higher productivity
- Accommodates up to 300 vials facilitating high throughput need for the lab
- Optional module enables accurate quantification of volatiles in samples with interfering matrices
その他のリソース
GC Headspace
Typical screening for blood alcohol is done with static headspace system hooked to a gas chromatograph equipped with dual FID detectors. Headspace analyzer which is an extraction & injection system, is equipped with GC split injector to modulate the amount of sample that goes into the analytical columns. A short uncoated guard column connects to two different columns typically using different stationary phases. The columns are connected to two different FIDs, one for quantitation, and the other for confirmation.
Blood is a very complex biological matrix, which includes salts, proteins, lipids and other metabolites. As such, a direct analysis of blood would result in a very frequent contamination of the GC injector, leading to frequent stops in the working routines and as such very low efficiency. Headspace facilitates the extraction of volatiles and a “clean” injection in the GC system leading to minimized instrument downtime and overall short time of analysis.
TRACE 1310 Headspace GC
TRACE 1300 series Headspace GC (includes 1300 and 1310):
- Instant connect modular GC
- Touch screen interface provides instant access for ease of use and local control
- Multiple detector for maximum confidence
- Multi-language capabilities
- Easy method development and effective feedback mechanism
- Full audit trail to maintain chain of custody
ISQ QD シングル四重極 GC-MS
ISQ series Single Quadrupole GC-MS (includes ISQ QD and ISQ LT):
- Robust, reliable solution for routine lab productivity
- Flexible configuration options to meet your lab volatile determination needs
- Extended dynamic range to cover range of volatiles
Achieve fast instrument setup for your analytical layout with the easy to install and swap Instant Connect modular injectors and detectors for the TRACE 1300 line of gas chromatography systems.
その他のリソース
消耗品
データ解析
Quick, efficient peak integration with dynamic data linkage and traceability to maintain steady tracking of data at any point during processing.
Chromeleon Software benefits:
- キャリブレーションプロットからレポート表やチャートまで、すべてのデータを動的に関連付け
- Compliance ready data management
- 完全なマルチユーザークロマトグラフィーマネージメントソリューション
- Robust and secure file access and storage
Learn how to streamline your laboratory workflow with Operational Simplity and Intelligent Functionality. Chromeleon 7.2 CDS は、クライアント/サーバー環境での定量質量分析のコントロールと処理が採用された初の CDS です。
その他のリソース
サンプルコレクション
Blood is collected by a phlebotomist in two grey-top tubes that should contain a preservative and anticoagulant (grey top tubes are normally used in medicine for glucose-testing). Typically sodium fluoride is used as preservative. One tube is for the crime lab to test and the other is preserved for the subject’s independent testing purposes. At the crime lab, one tube is opened and the contents are poured into a clean container. A measured amount of blood and a type of alcohol (usually n-propanol) as internal standard are mixed together into a headspace vial, which is then crimp-sealed.
Sample processing
The number of samples run in a day by a forensic lab includes specimen and quality control standards can easily reach several hundreds. Sample preparation often accounts for more than twice the time spent on actual chromatography. Automate and accelerate organic volatiles determinations to increase sample turnaround and lower the cost per analysis. It is paramount that the system will be able to run many unattended analysis and safely classify and store results.
TriPlus 300 Headspace Autosampler
The Thermo Scientific TriPlus 300 Headspace Autosampler: Designed for fast startup, high productivity, and maximum flexibility for any lab analyzing organic volatiles.
- Superior sample integrity and traceability
- End-to-end inertness of the sample path
- Seamless integration into multiple chromatography data systems
- A chromatographer-friendly local user interface further enhances the system's versatility
TriPlus RSH オートサンプラー
高度なロボットを内蔵し、きわめて高い精度やサンプル処理の柔軟性と、週末の無人運転が可能な信頼性を実現します。 Ideal for high throughput lab with quick sample turnaround time.
- Temperature-resistant syringe eliminates the need for a transfer line for direct headspace injection
- Overlapping sample incubation capability allows higher productivity
- Accommodates up to 300 vials facilitating high throughput need for the lab
- Optional module enables accurate quantification of volatiles in samples with interfering matrices
その他のリソース
GC Headspace
Typical screening for blood alcohol is done with static headspace system hooked to a gas chromatograph equipped with dual FID detectors. Headspace analyzer which is an extraction & injection system, is equipped with GC split injector to modulate the amount of sample that goes into the analytical columns. A short uncoated guard column connects to two different columns typically using different stationary phases. The columns are connected to two different FIDs, one for quantitation, and the other for confirmation.
Blood is a very complex biological matrix, which includes salts, proteins, lipids and other metabolites. As such, a direct analysis of blood would result in a very frequent contamination of the GC injector, leading to frequent stops in the working routines and as such very low efficiency. Headspace facilitates the extraction of volatiles and a “clean” injection in the GC system leading to minimized instrument downtime and overall short time of analysis.
TRACE 1310 Headspace GC
TRACE 1300 series Headspace GC (includes 1300 and 1310):
- Instant connect modular GC
- Touch screen interface provides instant access for ease of use and local control
- Multiple detector for maximum confidence
- Multi-language capabilities
- Easy method development and effective feedback mechanism
- Full audit trail to maintain chain of custody
ISQ QD シングル四重極 GC-MS
ISQ series Single Quadrupole GC-MS (includes ISQ QD and ISQ LT):
- Robust, reliable solution for routine lab productivity
- Flexible configuration options to meet your lab volatile determination needs
- Extended dynamic range to cover range of volatiles
Achieve fast instrument setup for your analytical layout with the easy to install and swap Instant Connect modular injectors and detectors for the TRACE 1300 line of gas chromatography systems.
その他のリソース
消耗品
データ解析
Quick, efficient peak integration with dynamic data linkage and traceability to maintain steady tracking of data at any point during processing.
Chromeleon Software benefits:
- キャリブレーションプロットからレポート表やチャートまで、すべてのデータを動的に関連付け
- Compliance ready data management
- 完全なマルチユーザークロマトグラフィーマネージメントソリューション
- Robust and secure file access and storage
Learn how to streamline your laboratory workflow with Operational Simplity and Intelligent Functionality. Chromeleon 7.2 CDS は、クライアント/サーバー環境での定量質量分析のコントロールと処理が採用された初の CDS です。
その他のリソース
リソース