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Custom Antibody Production
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Custom antibody services include competitively priced packages and purification options for antibody production. We provide complete support for all types and steps of antibody production—from peptide design, synthesis, and carrier protein conjugation to animal immunization, serum collection, hybridoma fusion, titer analysis, and final antibody purification. We can also immunize with recombinant proteins or protein fragments provided by you or expressed and purified in our lab to generate antibodies in rabbits, chickens, goats, guinea pigs, mice, or rats.
Our detailed knowledge of antigen-determining factors (e.g., size, localized secondary structure, and antigenicity) allows us to produce custom antibodies with unmatched specificity, affinity, and assay utility. Our expertise helps increase the probability that you will obtain a polyclonal or monoclonal antibody capable of distinguishing among even highly related proteins within complex mixtures to characterize protein expression patterns using IHC, ICC, IF, FACS, ELISA, western blotting, or immunoprecipitation (IP, Co-IP, ChIP).
Featured custom antibody categories
We offer eight protocol packages (immunization schedules for rabbits, goats, etc.) for producing custom polyclonal antibodies to peptide and protein antigens.
Custom antibody services for the production and purification of phosphospecific and post-translational-modification-specific antibodies.
Rat and mouse hybridoma development options for producing custom monoclonal antibodies from synthesized peptides or recombinant protein antigens.
Three-step purification procedure as well as specialized purification options for obtaining monospecific antibodies.
ELISA and additional screening and method validation procedures for custom antibodies.
The Thermo Scientific Antigen Profiler is a bioinformatics protein sequence analysis tool and custom peptide design algorithm for designing and creating superior peptide antigens. The platform incorporates an exclusive set of powerful bioinformatics algorithms for analyzing and designing peptide and protein antigens to ensure high-titer custom production of highly-specific antibodies. The analysis is offered as a service for researchers who wish to design and synthesize effective peptide antigens for custom antibody development using our comprehensive custom antibody production service.
Full-service, customized production of monoclonal and polyclonal antibodies
- Peptide design, synthesis, and conjugation—we will help you design an optimized antigenic peptide using our exclusive Thermo Scientific Antigen Profiler System. We will then synthesize the peptide for you and conjugate it to the carrier protein of your choice.
- Fusion protein expression and purification—we offer bacterial, mammalian, and insect protein expression services as part of our antibody development platform; we'll make your protein and purify it to prepare it for immunization and ELISA testing.
- Polyclonal or monoclonal antibody protocols—choose one of six species options (eight immunization schedules) for polyclonal antibody production, or one of three package sizes (number and strains of mice) for monoclonal antibody production.
- Biomarker antibody development—choose from several standardized packages for producing polyclonal or monoclonal antibodies to specific biomarkers of interest.
- Screening and titering analysis—we screen and characterize the antisera or hybridoma supernatants produced, and we will perform nearly any assay development and validation experiment you request.
- Purification specificities and deliverables—we purify all antibodies with an effective three-step procedure; in addition, we offer many specialized purification options for obtaining monospecific antibodies, such as to phosphorylated, acetylated, or other post-translational modification state.
Get started now:
Is this your first time considering custom antibody production? No problem!
- Study the Highlights and related informational pages above.
- Choose "Custom Antibody Request" and our form will guide you through the details.
Summary of service capabilities and options
The following tables summarize some of our more popular custom antibody production packages, options, and corresponding features.
Polyclonal antibody production services
| Recombinant Protein Antibody | Standard Anti-Peptide Antibody | Modification Specific Antibody† | |
|---|---|---|---|
| Antigen Design with Antigen Profiler | ✓ | ✓ | ✓ |
| Peptide Antigen Synthesis | N/A | ✓ | ✓ |
| Depleting Peptide Synthesis | N/A | N/A | ✓ |
| Protein Expression (vs. you supply) | Optional | N/A | N/A |
| Animal Immunization (8 protocol options) | ✓ | ✓ | ✓ |
| Bleeds, Serum Prep, ELISA Screening and Titration | ✓ | ✓ | ✓ |
| Affinity Purification and Depletion (several options) | Optional | Optional | ✓ |
| Specific Assay Validation or Antibody Labeling | Optional | Optional | Optional |
† Modification specific antibodies are phosphospecific antibodies or monospecific antibodies against peptides with other post-translational modifications or specific states (phosphorylation, sumoylation, myristoylation, acetylation, polymorphisms, drug binding, ubiquitination, glycosylation, isoforms, splice variants, ligand binding, prenylation, protein cleavage neo-epitopes, mutations, species cross-reactivity).
Monoclonal antibody production services
| Modification Specific Antibody† | Standard Anti-Peptide Antibody | Recombinant Protein Antibody | |
|---|---|---|---|
| Antigen Design with Antigen Profiler | ✓ | ✓ | ✓ |
| Peptide Antigen Synthesis | ✓ | ✓ | N/A |
| Control Peptide Synthesis | ✓ | N/A | N/A |
| Protein Expression (vs. you supply) | N/A | N/A | Optional |
| Animal Immunization (3 protocol options) | ✓ | ✓ | ✓ |
| Hybridoma Fusion, Cloning, Screening and ELISA Titration | ✓ | ✓ | ✓ |
| Culture, Production and Purification (several options) | Optional | Optional | Optional |
| Specific Assay Validation or Antibody Labeling | Optional | Optional | Optional |
| Complete Online Project Tracking | Coming Soon | Coming Soon | Coming Soon |
† Modification specific antibodies are phosphospecific antibodies or monospecific antibodies against peptides with other post-translational modifications or specific states (phosphorylation, sumoylation, myristoylation, acetylation, polymorphisms, drug binding, ubiquitination, glycosylation, isoforms, splice variants, ligand binding, prenylation, protein cleavage neo-epitopes, mutations, species cross-reactivity).
Polyclonal antibody production services
| Recombinant Protein Antibody | Standard Anti-Peptide Antibody | Modification Specific Antibody† | |
|---|---|---|---|
| Antigen Design with Antigen Profiler | ✓ | ✓ | ✓ |
| Peptide Antigen Synthesis | N/A | ✓ | ✓ |
| Depleting Peptide Synthesis | N/A | N/A | ✓ |
| Protein Expression (vs. you supply) | Optional | N/A | N/A |
| Animal Immunization (8 protocol options) | ✓ | ✓ | ✓ |
| Bleeds, Serum Prep, ELISA Screening and Titration | ✓ | ✓ | ✓ |
| Affinity Purification and Depletion (several options) | Optional | Optional | ✓ |
| Specific Assay Validation or Antibody Labeling | Optional | Optional | Optional |
† Modification specific antibodies are phosphospecific antibodies or monospecific antibodies against peptides with other post-translational modifications or specific states (phosphorylation, sumoylation, myristoylation, acetylation, polymorphisms, drug binding, ubiquitination, glycosylation, isoforms, splice variants, ligand binding, prenylation, protein cleavage neo-epitopes, mutations, species cross-reactivity).
Monoclonal antibody production services
| Modification Specific Antibody† | Standard Anti-Peptide Antibody | Recombinant Protein Antibody | |
|---|---|---|---|
| Antigen Design with Antigen Profiler | ✓ | ✓ | ✓ |
| Peptide Antigen Synthesis | ✓ | ✓ | N/A |
| Control Peptide Synthesis | ✓ | N/A | N/A |
| Protein Expression (vs. you supply) | N/A | N/A | Optional |
| Animal Immunization (3 protocol options) | ✓ | ✓ | ✓ |
| Hybridoma Fusion, Cloning, Screening and ELISA Titration | ✓ | ✓ | ✓ |
| Culture, Production and Purification (several options) | Optional | Optional | Optional |
| Specific Assay Validation or Antibody Labeling | Optional | Optional | Optional |
| Complete Online Project Tracking | Coming Soon | Coming Soon | Coming Soon |
† Modification specific antibodies are phosphospecific antibodies or monospecific antibodies against peptides with other post-translational modifications or specific states (phosphorylation, sumoylation, myristoylation, acetylation, polymorphisms, drug binding, ubiquitination, glycosylation, isoforms, splice variants, ligand binding, prenylation, protein cleavage neo-epitopes, mutations, species cross-reactivity).