Residual Protein Quantitation | Thermo Fisher Scientific

A breakthrough protein immuno-PCR quantitation platform designed for sensitive and robust protein quantitation with 4 orders of magnitude of dynamic range

The detection, characterization, and removal of process impurities are critical in biopharmaceutical production. Current methods to quantify proteins (e.g., ELISA) typically suffer from narrow dynamic range, low sensitivity, laborious workflows, lack of dilution linearity, and poor robustness when used with complex matrices. Numerous replicates are required to obtain valid results, while extensive dilutions to mitigate complex matrices further compromise sensitivity.

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Webinar Spotlight: Eliminate Barriers of Conventional Immunoassays with a Customizable qPCR-based High-Performance Assay Platform

In this webinar we talk about a system that combines semi-automated magnetic bead-based protein antigen extraction with quantitative real-time PCR as readout. Its many advantages in assay workflow, robustness, and technical superiority make it an ideal assay platform system for ELISA replacement and streamlined bioprocessing.

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The ProteinSEQ Protein Quantitation System is an immuno–PCR-based method that addresses these challenges, providing greater confidence and quality of data throughout the quantitation process. Quantitative PCR (qPCR) delivers outstanding sensitivity and broad dynamic range. The ProteinSEQ Protein Quantitation System is a breakthrough immunoassay platform that leverages the analytical benefits of TaqMan qPCR readout and the selectivity of immunochemistry.

PCR sensitivity: quantitation to as low as 0.2 ng/mL generic host cell protein (HCP)
Wide dynamic range: 4 orders of magnitude
Robustness: dilution linearity and efficiency in complex matrices
Semi-automation: 2-hour walk-away time

Technology
Software

The technology underlying ProteinSEQ Protein Quantitation Systems is based on the Applied Biosystems™ TaqMan™ Protein Expression Assay. This is a homogenous assay that utilizes two antibodies conjugated to an oligonucleotide via a streptavidin–biotin linkage. When the two antibodies bind to their protein target, their proximity permits ligation of their respective oligonucleotides, which can serve as templates for real-time PCR amplification and quantification (Figure 1).

Figure 1. TaqMan Protein Expression Assay Technology. The TaqMan Protein Expression Assay is a homogeneous assay based on two antibodies, each conjugated to an oligonucleotide through a streptavidin—biotin linkage. When the two conjugated antibodies bind to their target protein and are in proximity, the oligonucleotides can be ligated, serving as the template for real-time PCR amplification and quantification.

The software solution for Mycoplasma detection, protein analyses and residual DNA

The Applied Biosystems AccuSEQ Real-Time PCR Detection Software supports the unique needs of analytical testing of contaminants and impurities during the biopharmaceutical manufacturing process, as well as routine qPCR assays.

AccuSEQ software is part of the integrated workflow for Mycoplasma detection, residual Protein A quantitation, and residual DNA quantitation. Automated analysis tools enable one-click processing of complex C_t, amplitude (DV), and melting temperature for the MycoSEQ Mycoplasma detection assay according to user specifications. Nonlinear curve-fitting enables rapid analysis of ProteinSEQ Protein Quantitation System data. Next-generation algorithms deliver accurate quantitation data for the resDNASEQ Residual Host Cell DNA Quantitation.