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*Water solubility: With very few exceptions, all crosslinkers are soluble in miscible organic solvents such as DMSO, DMF, or acetonitrile. Typical protocols involve first dissolving the reagent at 10X the working concentration and then adding it to an aqueous reaction mixture. Organic solvents are not compatible with certain applications, and directly aqueous-soluble crosslinkers are preferable in these situations. We classify crosslinkers as water-soluble if they can be dissolved directly in water or aqueous buffers at greater than 1 to 5X the concentration needed for typical procedures.
**Spacer arm lengths: These are characterized as short (< 10 Å), medium (10.1–30 Å), or long (> 30 Å).
†Packaging: Single-use indicates that reagents are available in the unique No-Weigh format, which provides 1 or 2 milligrams of pre-weighed dry chemicals in a single-use vial. Just add water, buffer, or solvent to create a working solution. Premium grade indicates packaging for applications where product integrity and risk minimization are critical. These reagents have clearly defined quality standards by including batch-specific information such as quality assurance review, lot sample retention, and change control notification (CCN), as well as an enhanced level of analytical testing and product characterization.