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GeneArt® It… with GeneArt® Strings™ DNA Fragments
Why choose GeneArt® Strings™ DNA Fragments?
Conventional PCR and cloning techniques require optimization and troubleshooting, which take up valuable lab time and resources. What if you could have your favorite gene made for you, using a method that’s analogous to an optimized, error-free PCR reaction? Save time and money with GeneArt® Strings™ DNA Fragments—they’re molecular cloning made easy!
Video Testimonials
Customer Stories
James Lucas
Student
UC Davis Biochemistry
Member of the Grand Prize–winning team of the 2014 iGEM Competition
Why do you use GeneArt® Strings™ DNA Fragments, and how have they impacted your research?
For the UC Davis 2014 iGEM project, we needed to screen a large number of enzymes for unique substrate specificities. GeneArt® Strings™ fragments made it extremely straightforward to order our desired genes and construct our expression plasmids with Gibson assembly. This allowed us to minimize the time between identifying enzyme sequences and experimentally characterizing these enzymes in the lab. Within the timeframe of an iGEM project, accelerating this process was extremely important to the success of our project!
What do you like best about GeneArt® Strings™ DNA Fragments?
My favorite aspect of GeneArt® Strings™ fragments is how convenient it is to customize and order DNA. The online project manager lets you modify genes by adding 3’ and 5’ cloning sites, protecting against certain restriction sites, and optimizing sequences for expression in different chassis. The project manager interface is extremely intuitive and makes ordering GeneArt® Strings™ fragments quick and painless.
What was your ordering experience like?
Ordering GeneArt® Strings™ fragments is quick and efficient. It takes only about 10 minutes to order genes, and they arrive a week later ready for cloning.
Have you used the online GeneArt® portal for gene optimization?
Yes, the online portal for gene optimization is extremely easy to use. Once you are finished specifying cloning sites and restriction sites to avoid, optimization is as simple as selecting your chassis in a dropdown menu. It takes less than a minute and your genes are optimized and ready to go!
Once you received your GeneArt® Strings™ DNA Fragments, were you ready to proceed to your experiment?
On the same day as receiving my GeneArt® Strings™ fragments, I use Gibson assembly to construct my plasmids and transform them into BLR strainEscherichia coli for enzyme expression.
Would you recommend GeneArt® Strings™ DNA Fragments to others?
Definitely!
Other Quotes
Dr. Hans-Ulrich Schmoldt
BioNTech AG, Germany
Used GeneArt® Strings™ DNA Fragments for a 15-position alanine scan:
“I just wanted to give you feedback regarding your tip with String synthesis for an Alanine Scan. I must say I am really enthusiastic. I cloned the 15 Strings directly without PCR amplification and then sent 6 clones each for sequencing. Nearly all clones where correct, that means that I could finalize all clonings within 1.5 weeks.”
Citations for GeneArt® Strings™ DNA Fragments
- Dickson JR, Kruse C, Montagna DR et al. (2013) Alternative polyadenylation and miR-34 family members regulate tau expression. J Neurochem 127(6):739–749.
- Hartwig S, Frister T, Alemdar S et al. (2014) Expression, purification and activity assay of apatchoulol synthase cDNA variant fused to thioredoxin in Escherichia coli. Protein Expr Purif 97:61–71.
- Hitachi K, Nakatani M, Tsuchida K (2014) Myostatin signaling regulates Akt activity via the regulation ofmiR-486 expression. Int J Biochem Cell Biol 47:93–103.
- Hnilicova J, Jirat Matejckova J, Sikova M et al. (2014) Ms1, a novel sRNA interacting with the RNA polymerase core in mycobacteria. Nucleic Acids Res 42(18):11763–11776.
- Kunjapur AM, Tarasova Y, Prather KL (2014) Synthesis and accumulation of aromatic aldehydesin an engineered strain of Escherichia coli. Am J Chem Soc 136(33):11644–11654.
- Murai, M.J. et al. (2014) The same site on LEDGF IBD domain represents therapeutic target for MLL leukemia and HIV. Blood, ePub ahead of print.
- Ng FS, Schutte J, Ruau D et al. (2014) Constrained transcription factor spacing is prevalent and important for transcriptional control of mouse blood cells. Nucleic Acids Res 42(22):13513–13524.
- Oltean BM, Ernst M, Renneker S et al. (2013) Whole antigenic lysates of Ixodes ricinus, but not Der-p2 allergen-like protein, are potent inducers of basophil activation in previously tick-exposed human hosts. Transbound Emerg Dis 60 Suppl 2: 162–171.
- Parsons JB, Frank MW, Eleveld MJ et al. (2014) A thioesterase bypasses the requirement for exogenous fatty acids in the plsX deletion of Streptococcus pneumonia. Mol Microbiol ePub ahead of print.
- Seitz P, Pezeshgi Modarres H, Borgeaud S et al. (2014) ComEA is essential for the transfer of external DNA into the periplasm in naturally transformable Vibrio cholera cells. PLoS Genet 10(1):e1004066.
- Vyas S, Matic I, Uchima L et al. (2014) Family-wide analysis of poly(ADP-ribose) polymerase activity. Nature Commun 5:4426.
- Wright O, Delmans M, Stan GB et al. (2014) GeneGuard: a modular plasmid system designed for biosafety. ACS Synth Biol ePub ahead of print.
Fred Chedin
Associate Professor
UC Davis Genome Center
Molecular and Cellular Biology
Why do you use GeneArt® Strings™ DNA Fragments?
Our goal is to express human proteins in E. coli to purify them and characterize them biochemically. My experience is that traditional methods (produce a cDNA, clone it, validate it, try to express it) are lengthy and inefficient especially given the challenges of suboptimal codon usage between human and E. coli. GeneArt® Strings™ fragments allow us to bypass all these steps and clone in an optimized gene fragment that is expression-ready.
How have GeneArt® Strings™ DNA Fragments impacted your research?
Strings™ fragments have permitted a tremendous gain in productivity.
What do you like best about GeneArt® Strings™ DNA Fragments?
Ordering a custom-built gene fragment is made so easy. And the quality of the product is fantastic. One can go from receiving a Strings™ fragment to expressing the protein in a matter of 2–3 days.
How do GeneArt® Strings™ DNA Fragments compare to similar products?
I have not tested other companies. And I’m not about to—Strings™ fragments offer everything I need at a good price.
What was your ordering experience like?
Very smooth, easy, user-friendly.
Have you used the online GeneArt® portal for gene optimization?
Yes, and it works incredibly well.
Once you received your GeneArt® Strings™ DNA Fragments, were you ready to proceed to your experiment?
Yes, the fragments were cloned in a matter of 2 days, and successful protein expression was obtained another 2 days later.
Would you recommend GeneArt® Strings™ DNA Fragments to others?
Absolutely. I can’t conceive of going back to traditional cloning myself, given the gains in productivity that Strings™ fragments afford you.
Kathryn GuggenheimPostdoctoral fellow |
Why do you use GeneArt® Strings™ DNA Fragments?
For transformation into E. coli and expression of encoded protein of interest for characterization.
How have GeneArt® Strings™ DNA Fragments impacted your research?
They have saved weeks to months of busy work.
What do you like best about GeneArt® Strings™ DNA Fragments?
That they have saved weeks to months of busy work.
How do GeneArt® Strings™ DNA Fragments compare to similar products?
I have not ordered full genes from other companies before.
What was your ordering experience like?
Very easy and user-friendly ordering process.
Have you used the online GeneArt® portal for gene optimization?
Yes, it is very straightforward.
Once you received your GeneArt® Strings™ DNA Fragments, were you ready to proceed to your experiment?
Yes.
Would you recommend GeneArt® Strings™ DNA Fragments to others?
Yes.
Other Quotes
Dr. Hans-Ulrich Schmoldt
BioNTech AG, Germany
Used GeneArt® Strings™ DNA Fragments for a 15-position alanine scan:
“I just wanted to give you feedback regarding your tip with String synthesis for an Alanine Scan. I must say I am really enthusiastic. I cloned the 15 Strings directly without PCR amplification and then sent 6 clones each for sequencing. Nearly all clones where correct, that means that I could finalize all clonings within 1.5 weeks.”
James Lucas
Student
UC Davis Biochemistry
Member of the Grand Prize–winning team of the 2014 iGEM Competition
Why do you use GeneArt® Strings™ DNA Fragments, and how have they impacted your research?
For the UC Davis 2014 iGEM project, we needed to screen a large number of enzymes for unique substrate specificities. GeneArt® Strings™ fragments made it extremely straightforward to order our desired genes and construct our expression plasmids with Gibson assembly. This allowed us to minimize the time between identifying enzyme sequences and experimentally characterizing these enzymes in the lab. Within the timeframe of an iGEM project, accelerating this process was extremely important to the success of our project!
What do you like best about GeneArt® Strings™ DNA Fragments?
My favorite aspect of GeneArt® Strings™ fragments is how convenient it is to customize and order DNA. The online project manager lets you modify genes by adding 3’ and 5’ cloning sites, protecting against certain restriction sites, and optimizing sequences for expression in different chassis. The project manager interface is extremely intuitive and makes ordering GeneArt® Strings™ fragments quick and painless.
What was your ordering experience like?
Ordering GeneArt® Strings™ fragments is quick and efficient. It takes only about 10 minutes to order genes, and they arrive a week later ready for cloning.
Have you used the online GeneArt® portal for gene optimization?
Yes, the online portal for gene optimization is extremely easy to use. Once you are finished specifying cloning sites and restriction sites to avoid, optimization is as simple as selecting your chassis in a dropdown menu. It takes less than a minute and your genes are optimized and ready to go!
Once you received your GeneArt® Strings™ DNA Fragments, were you ready to proceed to your experiment?
On the same day as receiving my GeneArt® Strings™ fragments, I use Gibson assembly to construct my plasmids and transform them into BLR strainEscherichia coli for enzyme expression.
Would you recommend GeneArt® Strings™ DNA Fragments to others?
Definitely!
Other Quotes
Dr. Hans-Ulrich Schmoldt
BioNTech AG, Germany
Used GeneArt® Strings™ DNA Fragments for a 15-position alanine scan:
“I just wanted to give you feedback regarding your tip with String synthesis for an Alanine Scan. I must say I am really enthusiastic. I cloned the 15 Strings directly without PCR amplification and then sent 6 clones each for sequencing. Nearly all clones where correct, that means that I could finalize all clonings within 1.5 weeks.”
Citations for GeneArt® Strings™ DNA Fragments
- Dickson JR, Kruse C, Montagna DR et al. (2013) Alternative polyadenylation and miR-34 family members regulate tau expression. J Neurochem 127(6):739–749.
- Hartwig S, Frister T, Alemdar S et al. (2014) Expression, purification and activity assay of apatchoulol synthase cDNA variant fused to thioredoxin in Escherichia coli. Protein Expr Purif 97:61–71.
- Hitachi K, Nakatani M, Tsuchida K (2014) Myostatin signaling regulates Akt activity via the regulation ofmiR-486 expression. Int J Biochem Cell Biol 47:93–103.
- Hnilicova J, Jirat Matejckova J, Sikova M et al. (2014) Ms1, a novel sRNA interacting with the RNA polymerase core in mycobacteria. Nucleic Acids Res 42(18):11763–11776.
- Kunjapur AM, Tarasova Y, Prather KL (2014) Synthesis and accumulation of aromatic aldehydesin an engineered strain of Escherichia coli. Am J Chem Soc 136(33):11644–11654.
- Murai, M.J. et al. (2014) The same site on LEDGF IBD domain represents therapeutic target for MLL leukemia and HIV. Blood, ePub ahead of print.
- Ng FS, Schutte J, Ruau D et al. (2014) Constrained transcription factor spacing is prevalent and important for transcriptional control of mouse blood cells. Nucleic Acids Res 42(22):13513–13524.
- Oltean BM, Ernst M, Renneker S et al. (2013) Whole antigenic lysates of Ixodes ricinus, but not Der-p2 allergen-like protein, are potent inducers of basophil activation in previously tick-exposed human hosts. Transbound Emerg Dis 60 Suppl 2: 162–171.
- Parsons JB, Frank MW, Eleveld MJ et al. (2014) A thioesterase bypasses the requirement for exogenous fatty acids in the plsX deletion of Streptococcus pneumonia. Mol Microbiol ePub ahead of print.
- Seitz P, Pezeshgi Modarres H, Borgeaud S et al. (2014) ComEA is essential for the transfer of external DNA into the periplasm in naturally transformable Vibrio cholera cells. PLoS Genet 10(1):e1004066.
- Vyas S, Matic I, Uchima L et al. (2014) Family-wide analysis of poly(ADP-ribose) polymerase activity. Nature Commun 5:4426.
- Wright O, Delmans M, Stan GB et al. (2014) GeneGuard: a modular plasmid system designed for biosafety. ACS Synth Biol ePub ahead of print.
Fred Chedin
Associate Professor
UC Davis Genome Center
Molecular and Cellular Biology
Why do you use GeneArt® Strings™ DNA Fragments?
Our goal is to express human proteins in E. coli to purify them and characterize them biochemically. My experience is that traditional methods (produce a cDNA, clone it, validate it, try to express it) are lengthy and inefficient especially given the challenges of suboptimal codon usage between human and E. coli. GeneArt® Strings™ fragments allow us to bypass all these steps and clone in an optimized gene fragment that is expression-ready.
How have GeneArt® Strings™ DNA Fragments impacted your research?
Strings™ fragments have permitted a tremendous gain in productivity.
What do you like best about GeneArt® Strings™ DNA Fragments?
Ordering a custom-built gene fragment is made so easy. And the quality of the product is fantastic. One can go from receiving a Strings™ fragment to expressing the protein in a matter of 2–3 days.
How do GeneArt® Strings™ DNA Fragments compare to similar products?
I have not tested other companies. And I’m not about to—Strings™ fragments offer everything I need at a good price.
What was your ordering experience like?
Very smooth, easy, user-friendly.
Have you used the online GeneArt® portal for gene optimization?
Yes, and it works incredibly well.
Once you received your GeneArt® Strings™ DNA Fragments, were you ready to proceed to your experiment?
Yes, the fragments were cloned in a matter of 2 days, and successful protein expression was obtained another 2 days later.
Would you recommend GeneArt® Strings™ DNA Fragments to others?
Absolutely. I can’t conceive of going back to traditional cloning myself, given the gains in productivity that Strings™ fragments afford you.
Kathryn GuggenheimPostdoctoral fellow |
Why do you use GeneArt® Strings™ DNA Fragments?
For transformation into E. coli and expression of encoded protein of interest for characterization.
How have GeneArt® Strings™ DNA Fragments impacted your research?
They have saved weeks to months of busy work.
What do you like best about GeneArt® Strings™ DNA Fragments?
That they have saved weeks to months of busy work.
How do GeneArt® Strings™ DNA Fragments compare to similar products?
I have not ordered full genes from other companies before.
What was your ordering experience like?
Very easy and user-friendly ordering process.
Have you used the online GeneArt® portal for gene optimization?
Yes, it is very straightforward.
Once you received your GeneArt® Strings™ DNA Fragments, were you ready to proceed to your experiment?
Yes.
Would you recommend GeneArt® Strings™ DNA Fragments to others?
Yes.
Other Quotes
Dr. Hans-Ulrich Schmoldt
BioNTech AG, Germany
Used GeneArt® Strings™ DNA Fragments for a 15-position alanine scan:
“I just wanted to give you feedback regarding your tip with String synthesis for an Alanine Scan. I must say I am really enthusiastic. I cloned the 15 Strings directly without PCR amplification and then sent 6 clones each for sequencing. Nearly all clones where correct, that means that I could finalize all clonings within 1.5 weeks.”
GeneArt® It… with GeneArt® Gene Synthesis and Optimization
Have you ever lacked the time to clone your favorite gene? You should consider gene synthesis, sometimes referred to as synthetic biology. Whether you need industry-leading gene synthesis services or optimized protein expression, or want to outsource the entire process to protein and cell line production, GeneArt® products and services can help you succeed.
Customer Stories
Zmapp Story
Mapp Biopharmaceutical was seeking to improve expression of several monoclonal antibodies that are part of their Ebola therapy ZMappTM. With GeneArt® gene optimization, antibody yield was substantially enhanced by testing different nucleotide variants of the heavy and light chain genes. Gene synthesis and subcloning services provided by GeneArt® led to speedy evaluation of these variants.
Numerous global pharmaceutical companies rely on GeneArt® gene optimization and gene synthesis for enhancement of their protein therapeutics, predominantly monoclonal antibodies in mammalian cells. The collaboration with Mapp Biopharmaceutical, which manufactures ZMappTM in a relative of tobacco (Nicotiana benthamiana), proves that GeneArt® GeneOptimizer™ can also be used for plant expression.
Quote from Michael Pauly, CSO: “The excellent web interface, ease of ordering, great customer support and improved protein expression all make GeneArt® an important part of our drug development pipeline. We especially appreciate their willingness to respond very rapidly to our urgent requests for new reagents during the recent Ebola epidemic."
Photo in left was taken by Chandres (Own work) and is copyrighted. , via Wikimedia Commons. Note: This permission only extends to this work at this link http://commons.wikimedia.org/wiki/File:Nicotiana_benthamiana.jpg
This file is licensed under the creative commons Attribution-Share alike 3.0 Unported license.
GeneArt® It… with GeneArt® Strings™ DNA Fragments
Why choose GeneArt® Strings™ DNA Fragments?
Conventional PCR and cloning techniques require optimization and troubleshooting, which take up valuable lab time and resources. What if you could have your favorite gene made for you, using a method that’s analogous to an optimized, error-free PCR reaction? Save time and money with GeneArt® Strings™ DNA Fragments—they’re molecular cloning made easy!
Video Testimonials
Customer Stories
James Lucas
Student
UC Davis Biochemistry
Member of the Grand Prize–winning team of the 2014 iGEM Competition
Why do you use GeneArt® Strings™ DNA Fragments, and how have they impacted your research?
For the UC Davis 2014 iGEM project, we needed to screen a large number of enzymes for unique substrate specificities. GeneArt® Strings™ fragments made it extremely straightforward to order our desired genes and construct our expression plasmids with Gibson assembly. This allowed us to minimize the time between identifying enzyme sequences and experimentally characterizing these enzymes in the lab. Within the timeframe of an iGEM project, accelerating this process was extremely important to the success of our project!
What do you like best about GeneArt® Strings™ DNA Fragments?
My favorite aspect of GeneArt® Strings™ fragments is how convenient it is to customize and order DNA. The online project manager lets you modify genes by adding 3’ and 5’ cloning sites, protecting against certain restriction sites, and optimizing sequences for expression in different chassis. The project manager interface is extremely intuitive and makes ordering GeneArt® Strings™ fragments quick and painless.
What was your ordering experience like?
Ordering GeneArt® Strings™ fragments is quick and efficient. It takes only about 10 minutes to order genes, and they arrive a week later ready for cloning.
Have you used the online GeneArt® portal for gene optimization?
Yes, the online portal for gene optimization is extremely easy to use. Once you are finished specifying cloning sites and restriction sites to avoid, optimization is as simple as selecting your chassis in a dropdown menu. It takes less than a minute and your genes are optimized and ready to go!
Once you received your GeneArt® Strings™ DNA Fragments, were you ready to proceed to your experiment?
On the same day as receiving my GeneArt® Strings™ fragments, I use Gibson assembly to construct my plasmids and transform them into BLR strainEscherichia coli for enzyme expression.
Would you recommend GeneArt® Strings™ DNA Fragments to others?
Definitely!
Other Quotes
Dr. Hans-Ulrich Schmoldt
BioNTech AG, Germany
Used GeneArt® Strings™ DNA Fragments for a 15-position alanine scan:
“I just wanted to give you feedback regarding your tip with String synthesis for an Alanine Scan. I must say I am really enthusiastic. I cloned the 15 Strings directly without PCR amplification and then sent 6 clones each for sequencing. Nearly all clones where correct, that means that I could finalize all clonings within 1.5 weeks.”
Citations for GeneArt® Strings™ DNA Fragments
- Dickson JR, Kruse C, Montagna DR et al. (2013) Alternative polyadenylation and miR-34 family members regulate tau expression. J Neurochem 127(6):739–749.
- Hartwig S, Frister T, Alemdar S et al. (2014) Expression, purification and activity assay of apatchoulol synthase cDNA variant fused to thioredoxin in Escherichia coli. Protein Expr Purif 97:61–71.
- Hitachi K, Nakatani M, Tsuchida K (2014) Myostatin signaling regulates Akt activity via the regulation ofmiR-486 expression. Int J Biochem Cell Biol 47:93–103.
- Hnilicova J, Jirat Matejckova J, Sikova M et al. (2014) Ms1, a novel sRNA interacting with the RNA polymerase core in mycobacteria. Nucleic Acids Res 42(18):11763–11776.
- Kunjapur AM, Tarasova Y, Prather KL (2014) Synthesis and accumulation of aromatic aldehydesin an engineered strain of Escherichia coli. Am J Chem Soc 136(33):11644–11654.
- Murai, M.J. et al. (2014) The same site on LEDGF IBD domain represents therapeutic target for MLL leukemia and HIV. Blood, ePub ahead of print.
- Ng FS, Schutte J, Ruau D et al. (2014) Constrained transcription factor spacing is prevalent and important for transcriptional control of mouse blood cells. Nucleic Acids Res 42(22):13513–13524.
- Oltean BM, Ernst M, Renneker S et al. (2013) Whole antigenic lysates of Ixodes ricinus, but not Der-p2 allergen-like protein, are potent inducers of basophil activation in previously tick-exposed human hosts. Transbound Emerg Dis 60 Suppl 2: 162–171.
- Parsons JB, Frank MW, Eleveld MJ et al. (2014) A thioesterase bypasses the requirement for exogenous fatty acids in the plsX deletion of Streptococcus pneumonia. Mol Microbiol ePub ahead of print.
- Seitz P, Pezeshgi Modarres H, Borgeaud S et al. (2014) ComEA is essential for the transfer of external DNA into the periplasm in naturally transformable Vibrio cholera cells. PLoS Genet 10(1):e1004066.
- Vyas S, Matic I, Uchima L et al. (2014) Family-wide analysis of poly(ADP-ribose) polymerase activity. Nature Commun 5:4426.
- Wright O, Delmans M, Stan GB et al. (2014) GeneGuard: a modular plasmid system designed for biosafety. ACS Synth Biol ePub ahead of print.
Fred Chedin
Associate Professor
UC Davis Genome Center
Molecular and Cellular Biology
Why do you use GeneArt® Strings™ DNA Fragments?
Our goal is to express human proteins in E. coli to purify them and characterize them biochemically. My experience is that traditional methods (produce a cDNA, clone it, validate it, try to express it) are lengthy and inefficient especially given the challenges of suboptimal codon usage between human and E. coli. GeneArt® Strings™ fragments allow us to bypass all these steps and clone in an optimized gene fragment that is expression-ready.
How have GeneArt® Strings™ DNA Fragments impacted your research?
Strings™ fragments have permitted a tremendous gain in productivity.
What do you like best about GeneArt® Strings™ DNA Fragments?
Ordering a custom-built gene fragment is made so easy. And the quality of the product is fantastic. One can go from receiving a Strings™ fragment to expressing the protein in a matter of 2–3 days.
How do GeneArt® Strings™ DNA Fragments compare to similar products?
I have not tested other companies. And I’m not about to—Strings™ fragments offer everything I need at a good price.
What was your ordering experience like?
Very smooth, easy, user-friendly.
Have you used the online GeneArt® portal for gene optimization?
Yes, and it works incredibly well.
Once you received your GeneArt® Strings™ DNA Fragments, were you ready to proceed to your experiment?
Yes, the fragments were cloned in a matter of 2 days, and successful protein expression was obtained another 2 days later.
Would you recommend GeneArt® Strings™ DNA Fragments to others?
Absolutely. I can’t conceive of going back to traditional cloning myself, given the gains in productivity that Strings™ fragments afford you.
Kathryn GuggenheimPostdoctoral fellow |
Why do you use GeneArt® Strings™ DNA Fragments?
For transformation into E. coli and expression of encoded protein of interest for characterization.
How have GeneArt® Strings™ DNA Fragments impacted your research?
They have saved weeks to months of busy work.
What do you like best about GeneArt® Strings™ DNA Fragments?
That they have saved weeks to months of busy work.
How do GeneArt® Strings™ DNA Fragments compare to similar products?
I have not ordered full genes from other companies before.
What was your ordering experience like?
Very easy and user-friendly ordering process.
Have you used the online GeneArt® portal for gene optimization?
Yes, it is very straightforward.
Once you received your GeneArt® Strings™ DNA Fragments, were you ready to proceed to your experiment?
Yes.
Would you recommend GeneArt® Strings™ DNA Fragments to others?
Yes.
Other Quotes
Dr. Hans-Ulrich Schmoldt
BioNTech AG, Germany
Used GeneArt® Strings™ DNA Fragments for a 15-position alanine scan:
“I just wanted to give you feedback regarding your tip with String synthesis for an Alanine Scan. I must say I am really enthusiastic. I cloned the 15 Strings directly without PCR amplification and then sent 6 clones each for sequencing. Nearly all clones where correct, that means that I could finalize all clonings within 1.5 weeks.”
James Lucas
Student
UC Davis Biochemistry
Member of the Grand Prize–winning team of the 2014 iGEM Competition
Why do you use GeneArt® Strings™ DNA Fragments, and how have they impacted your research?
For the UC Davis 2014 iGEM project, we needed to screen a large number of enzymes for unique substrate specificities. GeneArt® Strings™ fragments made it extremely straightforward to order our desired genes and construct our expression plasmids with Gibson assembly. This allowed us to minimize the time between identifying enzyme sequences and experimentally characterizing these enzymes in the lab. Within the timeframe of an iGEM project, accelerating this process was extremely important to the success of our project!
What do you like best about GeneArt® Strings™ DNA Fragments?
My favorite aspect of GeneArt® Strings™ fragments is how convenient it is to customize and order DNA. The online project manager lets you modify genes by adding 3’ and 5’ cloning sites, protecting against certain restriction sites, and optimizing sequences for expression in different chassis. The project manager interface is extremely intuitive and makes ordering GeneArt® Strings™ fragments quick and painless.
What was your ordering experience like?
Ordering GeneArt® Strings™ fragments is quick and efficient. It takes only about 10 minutes to order genes, and they arrive a week later ready for cloning.
Have you used the online GeneArt® portal for gene optimization?
Yes, the online portal for gene optimization is extremely easy to use. Once you are finished specifying cloning sites and restriction sites to avoid, optimization is as simple as selecting your chassis in a dropdown menu. It takes less than a minute and your genes are optimized and ready to go!
Once you received your GeneArt® Strings™ DNA Fragments, were you ready to proceed to your experiment?
On the same day as receiving my GeneArt® Strings™ fragments, I use Gibson assembly to construct my plasmids and transform them into BLR strainEscherichia coli for enzyme expression.
Would you recommend GeneArt® Strings™ DNA Fragments to others?
Definitely!
Other Quotes
Dr. Hans-Ulrich Schmoldt
BioNTech AG, Germany
Used GeneArt® Strings™ DNA Fragments for a 15-position alanine scan:
“I just wanted to give you feedback regarding your tip with String synthesis for an Alanine Scan. I must say I am really enthusiastic. I cloned the 15 Strings directly without PCR amplification and then sent 6 clones each for sequencing. Nearly all clones where correct, that means that I could finalize all clonings within 1.5 weeks.”
Citations for GeneArt® Strings™ DNA Fragments
- Dickson JR, Kruse C, Montagna DR et al. (2013) Alternative polyadenylation and miR-34 family members regulate tau expression. J Neurochem 127(6):739–749.
- Hartwig S, Frister T, Alemdar S et al. (2014) Expression, purification and activity assay of apatchoulol synthase cDNA variant fused to thioredoxin in Escherichia coli. Protein Expr Purif 97:61–71.
- Hitachi K, Nakatani M, Tsuchida K (2014) Myostatin signaling regulates Akt activity via the regulation ofmiR-486 expression. Int J Biochem Cell Biol 47:93–103.
- Hnilicova J, Jirat Matejckova J, Sikova M et al. (2014) Ms1, a novel sRNA interacting with the RNA polymerase core in mycobacteria. Nucleic Acids Res 42(18):11763–11776.
- Kunjapur AM, Tarasova Y, Prather KL (2014) Synthesis and accumulation of aromatic aldehydesin an engineered strain of Escherichia coli. Am J Chem Soc 136(33):11644–11654.
- Murai, M.J. et al. (2014) The same site on LEDGF IBD domain represents therapeutic target for MLL leukemia and HIV. Blood, ePub ahead of print.
- Ng FS, Schutte J, Ruau D et al. (2014) Constrained transcription factor spacing is prevalent and important for transcriptional control of mouse blood cells. Nucleic Acids Res 42(22):13513–13524.
- Oltean BM, Ernst M, Renneker S et al. (2013) Whole antigenic lysates of Ixodes ricinus, but not Der-p2 allergen-like protein, are potent inducers of basophil activation in previously tick-exposed human hosts. Transbound Emerg Dis 60 Suppl 2: 162–171.
- Parsons JB, Frank MW, Eleveld MJ et al. (2014) A thioesterase bypasses the requirement for exogenous fatty acids in the plsX deletion of Streptococcus pneumonia. Mol Microbiol ePub ahead of print.
- Seitz P, Pezeshgi Modarres H, Borgeaud S et al. (2014) ComEA is essential for the transfer of external DNA into the periplasm in naturally transformable Vibrio cholera cells. PLoS Genet 10(1):e1004066.
- Vyas S, Matic I, Uchima L et al. (2014) Family-wide analysis of poly(ADP-ribose) polymerase activity. Nature Commun 5:4426.
- Wright O, Delmans M, Stan GB et al. (2014) GeneGuard: a modular plasmid system designed for biosafety. ACS Synth Biol ePub ahead of print.
Fred Chedin
Associate Professor
UC Davis Genome Center
Molecular and Cellular Biology
Why do you use GeneArt® Strings™ DNA Fragments?
Our goal is to express human proteins in E. coli to purify them and characterize them biochemically. My experience is that traditional methods (produce a cDNA, clone it, validate it, try to express it) are lengthy and inefficient especially given the challenges of suboptimal codon usage between human and E. coli. GeneArt® Strings™ fragments allow us to bypass all these steps and clone in an optimized gene fragment that is expression-ready.
How have GeneArt® Strings™ DNA Fragments impacted your research?
Strings™ fragments have permitted a tremendous gain in productivity.
What do you like best about GeneArt® Strings™ DNA Fragments?
Ordering a custom-built gene fragment is made so easy. And the quality of the product is fantastic. One can go from receiving a Strings™ fragment to expressing the protein in a matter of 2–3 days.
How do GeneArt® Strings™ DNA Fragments compare to similar products?
I have not tested other companies. And I’m not about to—Strings™ fragments offer everything I need at a good price.
What was your ordering experience like?
Very smooth, easy, user-friendly.
Have you used the online GeneArt® portal for gene optimization?
Yes, and it works incredibly well.
Once you received your GeneArt® Strings™ DNA Fragments, were you ready to proceed to your experiment?
Yes, the fragments were cloned in a matter of 2 days, and successful protein expression was obtained another 2 days later.
Would you recommend GeneArt® Strings™ DNA Fragments to others?
Absolutely. I can’t conceive of going back to traditional cloning myself, given the gains in productivity that Strings™ fragments afford you.
Kathryn GuggenheimPostdoctoral fellow |
Why do you use GeneArt® Strings™ DNA Fragments?
For transformation into E. coli and expression of encoded protein of interest for characterization.
How have GeneArt® Strings™ DNA Fragments impacted your research?
They have saved weeks to months of busy work.
What do you like best about GeneArt® Strings™ DNA Fragments?
That they have saved weeks to months of busy work.
How do GeneArt® Strings™ DNA Fragments compare to similar products?
I have not ordered full genes from other companies before.
What was your ordering experience like?
Very easy and user-friendly ordering process.
Have you used the online GeneArt® portal for gene optimization?
Yes, it is very straightforward.
Once you received your GeneArt® Strings™ DNA Fragments, were you ready to proceed to your experiment?
Yes.
Would you recommend GeneArt® Strings™ DNA Fragments to others?
Yes.
Other Quotes
Dr. Hans-Ulrich Schmoldt
BioNTech AG, Germany
Used GeneArt® Strings™ DNA Fragments for a 15-position alanine scan:
“I just wanted to give you feedback regarding your tip with String synthesis for an Alanine Scan. I must say I am really enthusiastic. I cloned the 15 Strings directly without PCR amplification and then sent 6 clones each for sequencing. Nearly all clones where correct, that means that I could finalize all clonings within 1.5 weeks.”
GeneArt® It… with GeneArt® Gene Synthesis and Optimization
Have you ever lacked the time to clone your favorite gene? You should consider gene synthesis, sometimes referred to as synthetic biology. Whether you need industry-leading gene synthesis services or optimized protein expression, or want to outsource the entire process to protein and cell line production, GeneArt® products and services can help you succeed.
Customer Stories
Zmapp Story
Mapp Biopharmaceutical was seeking to improve expression of several monoclonal antibodies that are part of their Ebola therapy ZMappTM. With GeneArt® gene optimization, antibody yield was substantially enhanced by testing different nucleotide variants of the heavy and light chain genes. Gene synthesis and subcloning services provided by GeneArt® led to speedy evaluation of these variants.
Numerous global pharmaceutical companies rely on GeneArt® gene optimization and gene synthesis for enhancement of their protein therapeutics, predominantly monoclonal antibodies in mammalian cells. The collaboration with Mapp Biopharmaceutical, which manufactures ZMappTM in a relative of tobacco (Nicotiana benthamiana), proves that GeneArt® GeneOptimizer™ can also be used for plant expression.
Quote from Michael Pauly, CSO: “The excellent web interface, ease of ordering, great customer support and improved protein expression all make GeneArt® an important part of our drug development pipeline. We especially appreciate their willingness to respond very rapidly to our urgent requests for new reagents during the recent Ebola epidemic."
Photo in left was taken by Chandres (Own work) and is copyrighted. , via Wikimedia Commons. Note: This permission only extends to this work at this link http://commons.wikimedia.org/wiki/File:Nicotiana_benthamiana.jpg
This file is licensed under the creative commons Attribution-Share alike 3.0 Unported license.
For Research Use Only. Not for use in diagnostic procedures.