No matter what the source of DNA, you will most likely need to use PCR to generate sufficient material for cloning. We offer best in-class PCR enzymes with brands you trust including our most popular enzymes for cloning (Table 1).

Hot-start Amplification of a Broad Range of Targets

AmpliTaq Gold® 360 DNA Polymerase (Roche) and Platinum® Taq DNA Polymerase

  • Increased specificity with choice of trusted hot-start technologies
  • Amplify the broadest range of targets (<3 kb)
  • Available in Master Mix or SuperMix formats for increased convenience

Chemically modified hot-start AmpliTaq Gold® 360 DNA Polymerase has been optimized to amplify a wide range of targets while delivering the high specificity, yield, and reliability you have come to expect.

Platinum® Taq DNA Polymerase uses antibody-mediated hot-start to enable increased specificity, yield, and sensitivity with the fastest activation times. Although there are several types of hot-start enzymes, the most reliable and widely used techniques involve chemically modified hot-start or antibody-mediated hot-start enzymes.

How to Choose Between Chemically Modified & Antibody-mediated Hot-start Reagents

Chemically modified hot-start reagents are best when performing high-throughput applications. These enzymes withstand longer periods at room temperature. However, the activation time is longer and the read lengths are shorter. Antibody-mediated hot-start products deliver fast activation times and minimal template degradation, which promote the amplification of longer fragments. However, antibody-mediated chemistries tend to exhibit reduced specificity and involve longer setup times.

With both types of hot-start technologies available from Life Technologies, you can choose the chemistry that works best for your research.

Robust PCR Aplification with High Yields & Increased Fidelity

Platinum® Taq DNA Polymerase High Fidelity

  • Greater than six times higher fidelity than Taq DNA polymerase
  • Amplification of fragments up to 20 kb
  • High yields with no need to optimize reaction conditions

The accuracy of Platinum® Taq DNA Polymerase High Fidelity is provided by a mixture of Platinum® Taq DNA Polymerase and the proofreading (3´ > 5´ exonuclease activity) enzyme from Pyrococcus species, GB-D polymerase. PCR specificity is improved with the incorporation of Platinum® automatic “hot-start” technology. Platinum® Taq DNA Polymerase High Fidelity is especially effective for high-fidelity applications such as cloning or mutagenesis.  Platinum® Taq DNA Polymerase High Fidelity predominantly produces PCR products with 3’-A overhangs for compatibility with TOPO® TA Cloning® Kits.

Which Standard PCR Enzyme Is Right for You?

  Value Choice for non-Hot Start DNA Polymerase Value Choice for non-Hot Start DNA Polymerase General Choice for Hot Start DNA Polymerase Premium Choice for Hot Start DNA Polymerase
Top Selleryesyes
Improved Specificityyesyes
Improved Sensitivityyes
Amplicon Size Up to 5 kbUp to 5 kbUp to 5 kbUp to 8 kb
Improved Yield yes
GC-Rich Formatyesyes
Activation Time Immediate Immediate 10 minutes 30 seconds
Master Mix/Super Mix Format yesyesyesyes
Price/Reaction$0.30 - $0.55 $0.44 - $0.55 $0.69
(4,000 Reactions) 		$0.72
(5,000 Reactions)
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