Sanger Sequencing Software Support – Getting Started

This software has been extensively tested on Windows® 7 SP1 (32-bit version). While there are some indications that the software will run normally on the 64-bit version of Windows® 7 SP1 operating system, the 64-bit configuration is not officially supported. It is strongly recommended that this software be run on Windows® 7 SP1 (32-bit).

If installing on the instrument computer, the update will require a new computer and upgrading the Data Collection Software as well. Please click here for the Sequencing Analysis Software 6 datasheet.

The computer requirements are as follows:

• Minimum: 2 GHz CPU or faster
• 2 GB RAM
• 1 GB hard disk space for application

Recommended CPU: Core 2 Duo E8400/3.0 GHz

• 4 GB RAM
• 1 GB hard disk space

For the operating system, Sequencing Analysis Software 6 requires Windows® 7 Professional, 32-bit edition, service pack 1. Please click here for the Sequencing Analysis Software 6 datasheet.

The Sequencing Analysis Software 6 is compatible with the following data files:

• 310 PC, Data Collection Software v.3.0, 3.1
  
• 3100 series, Data Collection Software v.1.0, 1.1, 2.0
  
• 3130 series, Data Collection Software v.3.0, 3.1, 4
  
• 3730 series, Data Collection Software v.3.0, 3.1, 4
  
• 3500 series, Data Collection Software v.1.0, 1.1, 2

For more information, please click here for the Sequencing Analysis Software 6 datasheet.

Unfortunately, Sequencing Analysis Software v.5.4 is not Windows® 7 compatible. Please click here for the Sequencing Analysis Software v.5.4 datasheet.

The computer requirements are as follows:

• Minimum: 2.8 GHz Pentium® IV CPU or faster
• 1 GB RAM
• 40 GB hard disk space for application

Recommended CPU: Core 2 Duo E8400/3.0 GHz

• 2 GB RAM
• 320 GB hard disk space
• For the operating system, Sequencing Analysis Software v.5.4 requires either Windows® XP Professional, service pack 2 or 3, or Windows Vista™ (32-bit Business Edition), service pack 1. Please click here for the Sequencing Analysis Software v.5.4 datasheet.

Sequencing Analysis Software v.5.4 is compatible with the following data files:
310 PC, Data Collection Software v.3.0, 3.1
3100 series, Data Collection Software v.1.0, 1.1, 2.0
3130 series, Data Collection Software v.3.0, 3.1, 4
3730 series, Data Collection Software v.3.0, 3.1, 4
3500 series, Data Collection Software v.1.0, v1.1, 2
For more information, please click here for the Sequencing Analysis Software v.5.4 datasheet.

Unfortunately, Sequencing Analysis Software v.5.3.1 is not Windows® 7 compatible. Please click here for the Sequencing Analysis Software v.5.3.1 datasheet.

The computer requirements are as follows:

• Minimum: 733 MHz Pentium® IV CPU or faster
• 512 MB RAM
• 5 GB hard disk space for application

For the operating system, Sequencing Analysis Software v.5.3.1 requires Windows® 2000, service pack 3 or 4, Windows® XP Professional, service pack 2 or 3, or Windows® Vista (32-bit Business Edition). Please click here for the Sequencing Analysis Software v.5.3.1 datasheet.

The Sequencing Analysis Software v.5.3.1 is compatible with the following data files:
310 PC, Data Collection Software v.3.0
3100 series, Data CollectionSoftware v,1.0, 1.1, 2.0
3130 series, Data CollectionSoftware v.3.0
3730 series, Data Collection Software v.3.0

For more information, please click here for the Sequencing Analysis Software v.5.3.1 datasheet.

If you received the error message about insufficient user privileges to start low level driver, you are most likely not logged in locally as the Administrator. Reboot the computer, change the domain to the local computer and log in as the Administrator.

Right click on “Computer” and select “Properties”. In the System section, look at the System Type and see if you have Windows® 7 Professional, 32-bit or 64-bit edition. Please write this down, as you will need this information during the install process.
To install the necessary driver:

1. Open a browser and go to https://www.ilok.com/#!home.
   
2. On the right-hand side of the page, you should see the “Free Download License Support” links.
   
   If your computer has the Windows® 7 Professional, 32-bit edition operating system, select the middle option (“Windows 32-bit XP, Vista, 7, 8”). If your computer has the Windows® 7 Professional, 64-bit edition operating system, choose the third option (“Windows 64-bit XP, Vista, 7, 8”).
   
   
   
   Note: If your browser gives you the choice to Run or Save, save the file. For the Internet Explorer® browser, you can choose the location to save the file. For Mozilla Firefox® and Google Chrome® browsers, the default location to save the file is the “Downloads” folder.
   
   
3. Run the installer. When the installation is complete, reboot the computer.

Right click on “Computer” and select “Properties”. In the System section, look at the System Type and see if you have Windows® 7 Professional, 32-bit or 64-bit edition. Please write this down, as you will need this information during the install process.
To install the necessary driver:

1. Open a browser and go to https://www.ilok.com/#!home.
2. On the right-hand side of the page, you should see the “Free Download License Support” links.
   
   If your computer has the Windows® 7 Professional, 32-bit edition operating system, select the middle option Windows 32-bit XP, Vista, 7, 8”). If your computer has the Windows® 7 Professional, 64-bit edition operating system, choose the third option (“Windows 64-bit XP, Vista, 7, 8”).
   
   
   
   Note: If your browser gives you the choice to Run or Save, Save the file. For the Internet Explorer® browser, you can choose the location to save the file. For Mozilla Firefox® and Google Chrome® browsers, the default location to save the file is the “Downloads” folder.
   
   
3. Run the installer. When the installation is complete, reboot the computer.

This software has been extensively tested on Windows® 7 SP1 (32-bit version). While there are some indications that software will run normally on the 64-bit version of Windows® 7 SP1 operating system, the 64-bit configuration is not officially supported. It is strongly recommended that this software is run on Windows® 7 SP1 (32-bit).

If installing on the instrument computer, the update will require a new computer and upgrading the Data Collection Software as well. Please click here for the SeqScape® Software v.3 datasheet.

The computer requirements are as follows:

• Minimum: 2.0 GHz or faster Intel® processor
• 2 GB RAM
• 1 GB hard disk space for application

Recommended CPU: Core 2 Duo E8400/3.0 GHz

• 4 GB RAM
• 1 GB hard disk space

For the operating system, SeqScape® Software v.3 requires Windows 7 Professional, 32-bit edition, service pack 1. Please click here for the SeqScape® Software v.3 datasheet.

The SeqScape® Software v.3 is compatible with the following data files:

• 310 PC, Data Collection Software v.3.0, 3.1
  
• 3100 series, Data Collection Software v.1.0, 1.1, 2.0
  
• 3130 series, Data CollectionSoftware v.3.0, 3.1, 4
  
• 3730 series, Data Collection Software v.3.0, 3.1, 4
  
• 3500 series, Data Collection Software v.1.0, 1.1, 2
  
• 3500 Dx series, Data Collection Software v.1.0

For more information, please click here for the SeqScape® Software v.3 datasheet.

This software is not Windows® 7 compatible. Please click here for the SeqScape® Software v.2.7 datasheet.

The computer requirements are as follows:

• Minimum: 2.8 GHz Pentium® IV CPU or faster
• 1 GB RAM
• 40 GB hard disk space for application
•  

Recommended CPU: Core 2 Duo E8400/3.0 GHz

• 2 GB RAM
• 320 GB hard disk space

For the operating system, SeqScape® Software v.2.7 requires either Windows® XP Professional, service pack 2 or 3 or Windows Vista™ (32-bit Business Edition), service pack 1. Please click here for the SeqScape® Software v.2.7 datasheet.

The SeqScape® Software v.2.7 is compatible with the following data files:

• 310 PC, Data Collection Software v.3.0, 3.1
  
• 3100 series, Data Collection Software v.1.0, 1.1, 2.0
  
• 3130 series, Data Collection Software v.3.0, 3.1, 4
  
• 3730 series, Data Collection Software v.3.0, 3.1, 4
  
• 3500 series, Data Collection Software v.1.0, 1.1, 2

Please note, upgrade will require Data Collection Software v.3.1 on the 3130 and 3730 series instruments for co-installation. System upgrade may be required.

For more information, please click here for the SeqScape® Software v.2.7 datasheet.

This software has been extensively tested on Windows® 7 SP1 (32-bit version). While there are some indications that the software will run normally on the 64-bit version of Windows® 7 SP1 operating system, the 64-bit configuration is not officially supported. It is strongly recommended that this software is run on Windows® 7 SP1 (32-bit).

If installing on the instrument computer, the update will require a new computer and upgrading the Data Collection Software as well. Please click here for the Variant Reporter® Software v.2 datasheet.

The computer requirements are as follows:

• Minimum: 2.0 GHz or faster Intel® processor
• 2 GB RAM
• 1 GB hard disk space for application

Recommended CPU: Core 2 Duo E8400/3.0 GHz

• 4 GB RAM
• 1 GB hard disk space

For the operating system, Variant Reporter® Software v.2 requires Windows® 7 Professional, 32-bit edition, service pack 1. Please click here for the Variant Reporter® Software v.2 datasheet.

The Variant Reporter® Software v.2 is compatible with the following data files:

• 3130 series Data Collection Software 4
• 3730 series Data Collection Software 4
• 3500 series Data Collection Software 20

For more information, please click here for the Variant Reporter® Software v.2 datasheet.

Unfortunately, Variant Reporter® Software v.1.1 is not Windows® 7 compatible.
Please click here for the Variant Reporter® Software v.1.1 datasheet.

The computer requirements are as follows:

• Minimum: 2.8 GHz Pentium® IV CPU or faster
• 512 MB RAM
• 40 GB hard disk space for application

Recommended CPU: Core 2 Duo E8400/3.0 GHz

• 1 GB RAM
• 320 GB hard disk space

For the operating system, Variant Reporter® Software v.1.1 requires either Windows® XP Professional, service pack 3, or Windows Vista™ (32-bit Business Edition), service pack 1. Please click here for the Variant Reporter® Software v.1.1 datasheet.

The Variant Reporter® Software v.1.1 is compatible with the following data files:

• 310 PC, Data Collection Software v.3.1
  
• 3130 series Data Collection Software v.3.1
  
• 3730 series Data Collection Software v.3.1
  
• 3500 series Data Collection Software v.1.0

Please note, ABI PRISM® 310 data will have to be pre-analyzed in Sequencing Analysis Software before importing into Variant Reporter®. For more information, please click here for the Variant Reporter® Software v.1.1 datasheet.

Yes, please see here.

Minor Variant Finder Software allows you to identify somatic mutations confidently down to 5% with 95% sensitivity and 99% specificity.

Minor Variant Finder Software subtracts sequencing background noise to allow for detection of rare minor variants.

Forward and reverse sequences for the sample, as well as forward and reverse sequences for a control sample with the same primary sequence are needed to analyze a sample in Minor Variant Finder Software.

Yes, the software allows reviewing of the original sequences of the test and control samples, as well as the noise-reduced sequence, all on the same page.

Variants can be exported in .vcf and .csv file formats.

Yes, Minor Variant Finder Software can be used to verify NGS data.