Search Thermo Fisher Scientific
Gene Expression Support
Explore the “Getting Started” and “Troubleshooting” sections for solutions to top inquiries and common problems. Browse through the “Guides and Tools” section to access comprehensive product-related support resources.
Getting Started
Troubleshooting
- I am getting amplification from my NTC wells. What is wrong?
- I am not getting any amplification from my sample. What is wrong?
- The amplification from this assay is later than what I expected. What could be wrong?
- What can I do if I cannot find a good endogenous control?
- How can I analyze my data if I do not have a calibrator/reference sample?
Guides and Tools
Search our database for Product Documents: Manuals, Product Literature, Citations and References
Search our database for Quality and Safety Documents: MSDS, COA
- TaqMan Gene Expression Assays brochure
- Overview: Gene Expression Analysis using TaqMan® Assays
- Download: OpenArray® TPF Plate Files
- Reference: Analysis of relative gene expression data using real-time quantitative PCR and the ddCT method (Livak and Schmittgen)
- Reference: Analyzing real-time PCR data by the comparative CT method (Schmittgen and Livak)
- Reference: Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays (Bustin)
- Video: Ask TaqMan® Video Gallery
- Video: Finding the Right TaqMan® Gene Expression Assay
- Video: qPCR Technology
- Video: Introduction to Symphoni™ Suite Analysis Software
- Video: Introduction to Thermo Fisher Cloud
- Video : Relative Quantification (RQ) Application Module on Thermo Fisher Cloud
Need sequences for your TaqMan Gene Expression Assays? Learn how to generate a padded amplicon
- Training: Gene Expression Data Analysis using ExpressionSuite Software
- Training: Overview of the Relative Quantitation (RQ) App on Thermo Fisher Cloud
- Browse our site for: Training, Events, and Webinars
- Download: OpenArray® TPF Plate Files
- Software: ExpressionSuite Software—for relative quantification data analysis
- Software: DataAssist™ Software—for relative quantification data analysis
- Software: Primer Express Software v3.0.1—for TaqMan® Probe and SYBR primer design
- Tool: OligoPerfect—free program for SYBR primer design
- Tool: TaqMan® Assay Search Tool
- Tool: Gene Expression Troubleshooting Tool
- Real-Time PCR: an app for real-time PCR resources, including videos, an interactive troubleshooting tool, and a reaction calculator
- Reagent Rewards: an app-based program that turns reagent purchases into rewards
Services
Custom Services and Reagents for Real-Time PCR (qPCR)
Our Genetic Analysis Custom Products and Services team offers a wide range of choices that include customized reagents such as master mixes or custom-designed TaqMan® assays. We will work with you to develop a custom solution that fits your needs.
Contract Laboratory Services for Real-Time PCR and other Genetic Analysis
Our Custom Products and Services team can handle large projects when you do not have access to all the right equipment or reagents. Our contract research work includes designing and performing experiments including sample preparation, data analysis, and reporting for many different applications.
For Research Use Only. Not for use in diagnostic procedures.