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Fluorescent Secondary Antibodies
Fluorescent-dye conjugated secondary antibodies provide a tool for identifying proteins in many applications including fluorescent cell imaging, western blotting, immunohistochemistry and more. The advantages of using a fluorescently labeled secondary antibody include brighter signal, multiplexing capabilities, and ease of use. Browse our portfolio of Alexa Fluor secondary antibodies, classic secondaries (such as FITC and TRITC), and our selection of enzyme and biotin-labeled secondaries.
Need to select the optimal fluorescent dyes for use with the EVOS cell imaging systems? The Fluorophore and Reagent Selection Guide for Cell Imaging is here to help!
Quick links to secondary antibody conjugate products
| Alexa Fluor Secondary Antibody Conjugates | Other Fluorescent Secondaries | Enzyme & Biotin-Labeled Secondaries |
|---|---|---|
Quick links to fixatives, mountants and other organelle staining reagents for use with secondary antibodies
| Reagent type | Recommended products |
|---|---|
| Mountant | |
| Fixative | |
| Organelle staining |
Compare fluorophore conjugate technologies
See how classical fluorophore, Alexa Fluor, and Alexa Fluor Plus secondary antibody conjugates compare.
| Classical fluorophore conjugates | Alexa Fluor conjugates | Alexa Fluor Plus conjugates | |
|---|---|---|---|
| What conjugate products are available? | APC Cy3 Cy5 FITC PE TRITC Rhodamine Texas Red Texas Red-X | Conjugates of 18 Alexa Fluor dyes | Conjugates of 7 Alexa Fluor Plus dyes |
| Use | For detection of high-abundance targets For use with easy-to-obtain samples | For detection of medium- to high-abundance targets For use in most research areas | For detection of low-abundance targets For use with hard-to-obtain samples, e.g., neuroscience |
| Applications |
| ||
| Signal-to-noise ratio | Low | Medium | High |
| Photostability | Varying levels of photostability, depending on dye | Photostable | Photostable |
| Specificity | Various options available | Various options available | Highly specific, low cross-reactivity (highly cross-adsorbed) |
| Sensitivity and brightness | Lower sensitivity and brightness compared to leading Alexa Fluor conjugates | Widely recognized standard for brightness and sensitivity | Improved sensitivity and brightness over leading Alexa Fluor conjugates in IF/ICC imaging and fluorescent western blotting |
| Range of linear detection | Low | Medium | High |
Featured secondary antibody conjugate categories
Select from 18 different individual Alexa Fluor dyes and 4 Alexa Fluor tandem dyes as secondary antibody conjugates. Alexa Fluor secondary antibodies exhibit superior brightness and photostability, outperforming conventional fluorescent secondary antibodies.
Learn about Alexa Fluor Plus secondary antibodies, which represent an advancement in fluorescent conjugate technology, designed to provide brighter signal, enhanced sensitivity, and minimal cross-reactivity in a variety of applications.
Discover features and varieties of available horseradish peroxidase (HRP), alkaline phosphatase (AP), biotin, and other enzyme or affinity-tagged secondary antibody conjugates.
Determine which Alexa Fluor secondary antibodies fit your imaging or western blotting needs by using our instrument compatibility table to guide you. Microscope, microplate reader, high-content platform and fluorescent western blotting compatibility information are also provided.
For Research Use Only. Not for use in diagnostic procedures.