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Flow Compatible Positive Isolation Kits |
Positive cell isolation directly from human whole blood, buffy coat, or mouse/human mononuclear cells
Flow cytometry and fluorescent-activated cell sorting (FACS) heavily rely on the user working with intact cells. Flow researchers must balance removal of cell debris and unwanted cells while preserving the integrity of cells-of-interest. Dynabeads FlowComp positive cell isolation kits positively isolate cells of interest directly followed by cell release and removal of magnetic beads. No sample preparation (e.g., density gradient centrifugation or lysis of red blood cells) is required, which helps eliminate the risk of contaminating and incubating your cells with iron, dextran, or other potentially harmful chemicals that are present in lysis buffers.
Get flow-ready cells in just three steps:
- Add FlowComp antibody to sample for binding of target T cells
- Add magnetic beads to capture targeted T cells
- T cells are released from magnetic beads—ready for flow or any other downstream application
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Dynabeads magnetic beads combine with T cell markers to positively isolate specific T cell populations of interest. FlowComp positive isolation kits are available against four different human T cell markers.
- CD3: targets cytotoxic T cells (CD8+ naive T cells) and T helper cells (CD4+ naive T cells)
- CD4: targets T helper (Th) cells, regulatory T (Treg) cells, and natural killer T (NKT) cells
- CD8: targets cytotoxic T cells (CTLs)
- CD14: targets naive CD4+ T cells
Cells isolated with Dynabeads FlowComp kits keep their functional characteristics and are directly suitable for flow cytometry and any downstream cell-based assay.
View FlowComp protocol for isolation from human whole blood
Order Dynabeads FlowComp kits for human T cell markers
Dynabeads magnetic beads combine with T cell markers to positively isolate your specific T cell populations of interest for your mouse studies. FlowComp positive isolation kits are available against four different mouse T cell markers.
- CD4: targets naive CD4+ T cells
- CD8: targets cytotoxic T cells (CD8+ naive T cells)
- CD4/CD25: targets regulatory CD4+ T cells
- Pan T cell: targets all T cells
Cells isolated with Dynabeads FlowComp kits are significantly purer and more viable than isolation with a column-based method (Figure 1). These cells keep their functional characteristics and are suitable to be used directly for flow cytometry and any downstream cell-based assay.
Click image to enlarge
Figure 1. Comparison of cell purity and viability using Dynabeads FlowComp mouse CD4 marker vs a column-based method for mouse CD4 T cells. The top panel is representative of control for cell purity and viability before cell isolation. Using the Dynabeads FlowComp mouse CD4 marker shows a 97.2% pure population in the left panel and 86% cell viability in the right panel. After isolation with a column-based method for mouse CD4 T cells, cells were 78.5% pure population in the left panel and 63% cell viability in the right panel.
Dynabeads FlowComp build-your-own isolation kit
Combine Dynabeads magnetic beads with your antibody of choice to isolate any cell type. The Dynabeads FlowComp Flexi Kit has a wide range of species reactivity. Starting samples are compatible with the build-your-own kit:
- Whole blood
- Peripheral blood mononuclear cells/mononuclear cells (PBMC/MNC)
- Single cell suspensions from lymph nodes, spleen, or other tissues
Dynabeads FlowComp kit ordering information
 | Compatible antibodies with FlowComp Dynabeads human CD4 positive isolation kit |
| Conjugate | Recommended antibody |
|---|---|
| Alexa Fluor 405 | CD4, Mouse anti-Human, (Alexa Fluor 405) |
| Alexa Fluor 488 | CD4, Mouse anti-Human, (Alexa Fluor 488) |
| Alexa Fluor 700 | CD4, Mouse anti-Human, (Alexa Fluor 700) |
| APC | CD4, Mouse anti-Human, (APC) |
| APC-Alexa Fluor 750 | CD4, Mouse anti-Human, (APC-Alexa Fluor 750) |
| APC-Cy5.5 | CD4, Mouse anti-Human, (APC-Cy5.5) |
| FITC | CD4, Mouse anti-Human, (FITC) |
| FITC | CD4, Mouse anti-Human, (FITC) |
| Pacific Blue | CD4, Mouse anti-Human, (Pacific Blue) |
| Pacific Orange | CD4, Mouse anti-Human, (Pacific Orange) |
| PE | CD4, Mouse anti-Human, (PE) |
| PE | CD4, Mouse anti-Human, (PE) |
| PE-Alexa Fluor 610 | CD4, Mouse anti-Human, (PE-Alexa Fluor 610) |
| PE-Alexa Fluor 700 | CD4, Mouse anti-Human, (PE-Alexa Fluor 700) |
| PE-Cy5.5 | CD4, Mouse anti-Human, (PE-Cy5.5) |
| PE-Cy7 | CD4, Mouse anti-Human, (PE-Cy7) |
| PE-Texas Red | CD4, Mouse anti-Human, (PE-TR) |
| TRI-COLOR | CD4, Mouse anti-Human, (TRI-COLOR) |
 | Compatible antibodies with FlowComp Dynabeads mouse CD4 positive isolation kit |
| Conjugate | Recommended antibody |
|---|---|
| Alexa Fluor 405 | CD4, Rat anti-Mouse, (Alexa Fluor 405) |
| Alexa Fluor 488 | CD4, Rat anti-Mouse, (Alexa Fluor 488) |
| APC | CD4, Rat anti-Mouse, (APC) |
| APC-Cy5.5 | CD4, Rat anti-Mouse, (APC-Cy5.5) |
| FITC | CD4, Rat anti-Mouse, (FITC) |
| Pacific Blue | CD4, Rat anti-Mouse, (Pacific Blue) |
| Pacific Orange | CD4, Rat anti-Mouse, (Pacific Orange) |
| PE | CD4, Rat anti-Mouse, (PE) |
| PE-Alexa Fluor 610 | CD4, Rat anti-Mouse, (PE-eFluor 610) |
| PE-Texas Red | CD4, Rat anti-Mouse, (PE-Texas Red) |
| TRI-COLOR | CD4, Rat anti-Mouse, (TRI-COLOR) |
| Compatible antibodies with FlowComp Dynabeads human CD8 positive isolation kit |
| Conjugate | Recommended antibody |
|---|---|
| Alexa Fluor 405 | CD8, Mouse anti-Human, (Alexa Fluor 405) |
| Alexa Fluor 647 | CD8, Mouse anti-Human, (Alexa Fluor 647) |
| Alexa Fluor 700 | CD8, Mouse anti-Human, (Alexa Fluor 700) |
| APC | CD8, Mouse anti-Human, (APC) |
| APC-Alexa Fluor 750 | CD8, Mouse anti-Human, (APC-Alexa Fluor 750) |
| FITC | CD8, Mouse anti-Human, (FITC) |
| FITC | CD8, Mouse anti-Human, (FITC) |
| Pacific Blue | CD8, Mouse anti-Human, (Pacific Blue) |
| Pacific Orange | CD8, Mouse anti-Human, (Pacific Orange) |
| PE | CD8, Mouse anti-Human, (PE) |
| PE | CD8, Mouse anti-Human, (PE) |
| PE-Alexa Fluor 610 | CD8, Mouse anti-Human, (PE-Alexa Fluor 610) |
| PE-Cy5.5 | CD8, Mouse anti-Human, (PE-Cy5.5) |
| PE-Cy7 | CD8, Mouse anti-Human, (PE-Cy7) |
| PE-Texas Red | CD8, Mouse anti-Human, (PE-Texas Red) |
| TRI-COLOR | CD8, Mouse anti-Human, (TRI-COLOR) |
For information about tested clones from other suppliers than Thermo Fisher, please contact technical support.
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