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We have collaborated with The Parkinson’s Institute in Sunnyvale, California, to demonstrate how to develop cellular models to identify drugs that may ameliorate the underlying processes of Parkinson’s disease (PD). In a series of white papers, we described three steps for creating PD cell models using Life Technologies tools for generation of NSCs, iPSCs, and editing the genome. We have collected a set of resources here to help enable your disease modeling research.
Protocols and user guides on this page | More resources on this page |
What assay? | Workflow step | What consumable? | Protocol or user guide |
---|---|---|---|
iPSC culture | Culture | KnockOut™ Serum Replacement | Feeder-Dependent Culture of Human Induced Pluripotent Stem Cells |
iPSC culture | Culture | Essential 8™ Medium | Culturing Pluripotent Stem Cells (PSCs) in Essential 8™ Medium |
iPSC culture | Culture | mouse embryonic fibroblasts | Culturing Induced Pluripotent Stem Cells (iPSCs) on MEF Conditioned Medium |
hESC culture | Culture | mouse embryonic fibroblasts | Culturing Induced Pluripotent Stem Cells (hESCs) on MEF Conditioned Medium |
NSC culture | Culture | Neural Expansion Medium | Expansion of Neural Stem Cells Using Neural Expansion Medium |
NSC culture | Culture | StemPro® NSC SFM | Expansion of Neural Stem Cells Using StemPro® NSC SFM |
What assay? | Workflow step | What instrument? | What consumable? | Protocol or user guide |
---|---|---|---|---|
iPSC generation | Engineer | EVOS® XL Imaging System | CytoTune®-iPS Sendai Reprogramming Kit | Reprogramming Fibroblasts with the CytoTune®-iPS Reprogramming Kit |
iPSC reprogramming efficiency calculation | Engineer | EVOS® FLoid® Cell Imaging Station | CytoTune®-iPS Sendai Reprogramming Kit | Calculating Reprogramming Efficiency using CytoTune®-iPS Reprogramming Kit |
Genomic cleavage | Engineer | Agarose gel electrophoresis | GeneArt® Genomic Cleavage Detection Kit | GeneArt® Genomic Cleavage Detection Kit - User Guide |
SNP genotyping | Engineer | QuantStudio® 12K Flex Real-Time PCR System | Custom TaqMan® SNP Genotyping Assays | Custom TaqMan® SNP Genotyping Assays - Protocol |
Targeted DNA double-stranded breakage via electroporation | Engineer | Neon® Transfection System | GeneArt® TALs Products and Services | GeneArt® Precision TALs - User Manual |
What assay? | Workflow step | What instrument? | What consumable? | Protocol or user guide |
---|---|---|---|---|
iPSC characterization - EB formation immunostaining | Differentiate | EVOS® FLoid® Cell Imaging Station | antibodies | Creation of Embryoid Bodies from iPSCs |
NSC generation | Differentiate | EVOS® XL Imaging System | PSC Neural Induction Medium | Induction of Neural Stem Cells Using Gibco® PSC Neural Induction Medium |
NSC differentiation - DA neuron | Differentiate | EVOS® FLoid® Cell Imaging Station | 50X B-27® Serum-Free Supplement, 100X N-2 Supplement | Differentiating Neural Stem Cells into Neurons |
NSC differentiation - glial | Differentiate | EVOS® FLoid® Cell Imaging Station | 50X B-27® Serum-Free Supplement, 100X N-2 Supplement | Differentiating Neural Stem Cells into Glial Cells |
All webinars were recorded at the 24 Hours of Stem Cells Event, November 2013.
Date | Title | |
---|---|---|
On Demand | Life Technologies and The Parkinson's Institute collaborate to understand the molecular basis for Parkinson's disease | View Webinar |
On Demand | Generation of induced pluripotent stem cells from Parkinson's disease fibroblasts | View Webinar |
On Demand | Generation and characterization of neural stem cells from Parkinson's disease donor-derived iPSCs | View Webinar |
On Demand | Genome editing of Parkinson's disease iPSCs using GeneArt® Precision TALs technology | View Webinar |
Video | Presented | Year |
---|---|---|
Creating Integration-free iPSCs and NSCs to Model Parkinson’s Disease by Dr. Birgitt Schuele | ISSCR | 2013 |