Search Thermo Fisher Scientific
QuantiGene Plex Gene Expression Assay
Accurate and precise RNA quantitation—measure up to 80 genes in a single well
QuantiGene Plex assays provide a fast and high-throughput solution for multiplexed gene expression quantitation, allowing the simultaneous measurement of up to 80 genes of interest in a single well of a 96- or 384-well plate. The QuantiGene Plex assay is hybridization-based and incorporates branched DNA (bDNA) technology, which uses signal amplification rather than target amplification for direct measurement of RNA transcripts. The assay is extremely easy to use with a simple ELISA-like workflow, and does not require RNA purification.
NEW Invitrogen Immune Response 80-Plex Human QuantiGene Plex Panel
Analyze 80 cytokine, chemokine, and growth factor RNA targets simultaneously for efficient immune response profiling, biomarker discovery, and validation.
NEW Use the Invitrogen QuantiGene Plex Gene Expression Panel
Browse hundreds of preconfigured and published panels using name, keyword, category, or gene target search. You can also create custom panels.
Unique benefits of QuantiGene Plex Gene Expression Assay
- Increase efficiency—harmonized gene and protein multiplex solution—QuantiGene and ProcartaPlex matched panels are complementary, highly correlative and run on the same Luminex platform, seamlessly integrated for unparalleled insights
- Excellent correlation with gold standard technology—between qPCR and QuantiGene
- Works with difficult sample types—works with degraded and cross-linked RNA in FFPE tissues and directly with blood; no RNA purification required
- True multiplexing—measure up to 80 genes of interest and housekeeping genes in the same well with no cross-reactivity, helping reduce the number of samples needed
- Standardized platform—96-well plate format compatible with Luminex 200, MAGPIX, and FlexMap3D systems and 384-well with FlexMap3D system
- Simple workflow—ELISA-like workflow for target hybridization and signal amplification with no reverse transcription needed
- Large inventory of validated genes—select from over 22,000 genes to create pathway- and disease-themed panels
- Customization—if we don’t have your gene(s), we can create your custom panel within 3–4 weeks
How QuantiGene Plex Assay works
The QuantiGene Plex Assay utilizes xMAP Luminex beads for multiplexing of 3 to 80 RNA targets followed by branched DNA-based signal amplification. The assay can be performed in 96- or 384-well plates, and signal is detected using a Luminex instrument.
Video: QuantiGene Plex–How It Works animation
QuantiGene Plex Assays incorporate branched DNA technology and Luminex xMAP technology for accurate multiplex gene expression profiling. Branched DNA assays allow for the direct measurement of RNA transcripts by using signal amplification rather than target amplification.
QuantiGene Plex Gene Expression Assay workflow
The QuantiGene Plex Gene Expression Assay is a hybridization-based assay using xMAP Luminex beads and performed in 96- or 384-well plates. The assay is based on direct quantification of the RNA targets for multiplexing of 3 to 80 RNA targets and branched DNA (bDNA) signal amplification technology. On the first day the sample is lysed to release the RNAs and incubated overnight with target specific probe sets and Luminex capture beads. On the second day the signal amplification tree is built via sequential hybridization of Pre-amplifier, Amplifier, and Label Probe. Each amplification unit provides a 400x signal amplification and there are six amplification units per target RNA copy resulting in a 2,400x signal amplification per copy RNA. The signal is detected by using the fluorescent reporter molecule, phycoerythrin, on a Luminex instrument for readout and analysis.
QuantiGene Plex Assay Workflow
Step 1.Sample preparation: Samples are lysed to release and stabilize RNAs. The RNA assay works with a variety of samples such as: cultured cells, virus, bacteria, plant and animal tissues, FFPE tissues, whole blood and PAXGene blood, or purified RNA.
Step 2. Target hybridization: Overnight hybridization in the 96- or 384-well plates with the target specific probe sets panel (Capture extenders–CEs, Label Extenders–LEs, and Blocking probes).
Step 3. Signal amplification: Signal amplification is achieved using branched DNA (bDNA) technology. First, an individual Pre-Amplifier molecule hybridizes to each pair of Label Extenders. Then, multiple Amplifier molecules hybridize to each Pre-Amplifier. Finally, multiple biotinylated Label Probe oligonucleotides hybridize to each Amplifier.
Step 4. Detection: Addition of streptavidin phycoerythrin (SAPE) generates a signal that is proportional with the amount of target RNA present in the sample. The signal is read using a Luminex instrument.
Click image to enlarge
Step 5. Data Analysis: Analyze data using the Thermo Fisher Connect cloud-based QuantiGene data analysis application from any computer, anywhere. For advanced analysis and visualization, data can be analyzed using the free desktop application software, Applied Biosystems Transcriptome Analysis Console (TAC) software. TAC provides visualization and interpretation using tools such as scatter and volcano plots, hierarchical clustering, and provides link outs to publicly available annotations.
QuantiGene Plex high throughput assays
The QuantiGene Plex 384-well assay format enables multiplex gene expression analysis in a high throughput format. The assay uses target-specific probes to capture the RNA of interest and branched DNA technology to amplify fluorescent signals which are read with the FLEXMAP 3D, Luminex platform. Automation and batch processing options for this assay format increase sample throughput and decrease hands-on time while allowing for fast time to results for screening projects.
The QuantiGene Plex 384-well Assay Kit (Cat. No. QP1016) contains all the necessary reagents, buffers, and plates to perform QuantiGene Plex assays in 384-well plates. Design your target-specific QuantiGene Plex panel with our Panel Configuration Tool or contact your local Technical Sales Specialist for more information.
QuantiGene Plex 384-well example data
The QuantiGene Plex 384-well assay is designed to increase sample throughput and decrease hands-on time while allowing for fast time to results for screening projects. Below is example data that was generated using the 384-well format using the recommended equipment listed below.
Stimulation of cytokine production in HeLa cells
Cytokine production in cultured HeLa cells was stimulated using a Cell Stimulation Cocktail and monitored for various time points up to 24 hours. In this experiment, a full 80-plex panel was used for screening purposes focused on cytokine, and chemokine biomarkers.
Click image to enlarge
Figure 1. Normalized gene expression of various targets in HeLa cells at different time points post stimulation with a Cell Stimulation cocktail. Y-axis: Average normalized expression; x-axis: mRNA targets; z-axis: time points of stimulation. Only a subset of gene targets tested is being displayed. HeLa cells were cultured in a 384-well format and stimulated with a Cell Stimulation cocktail for various times up to 24h (1h, 2h, 4h, 24h). Unstimulated cells served as baseline control. At the indicated time points, cells were lysed according to the QuantiGene sample prep protocol for cell lysates using liquid handling (Thermo Fisher Platemate). After all samples from all time points were prepared, the QuantiGene Plex 384-well assay was performed. Chart illustrates the normalized gene expression results.
The normalized gene expression of various targets in HeLa cells at different time points post stimulation with a Cell Stimulation cocktail reveals the degree of up- or downregulation. The average normalized expression (y-axis) represents the level of gene induction for the mRNA targets listed on the x-axis, while the time points of stimulation are shown on the z-axis. Of note, for example IL1B, IL8 and TGFB1 show pronounced relative gene expression at various time points of stimulation. While TGFB1 relative expression seems to gradually increase over time, peak expression for IL1B is seen at later time points around 24h. Table 1 shows normalized gene expression and fold change of various targets in HeLa cells at different time points post stimulation with a Cell Stimulation cocktail.
Click image to enlarge
Table 1 and 2. Normalized gene expression and fold change of various targets in HeLa cells at different time points post stimulation with a Cell Stimulation cocktail.
See how scientists are using QuantiGene Plex 384-well in their research:
- Danahay et. al. (2015) Notch2 Is Required for Inflammatory Cytokine-Driven Goblet Cell Metaplasia in the Lung. Cell Reports 10:239-252.
- Ferrer et al. (2014) A multiplex high-throughput gene expression assay to simultaneously detect disease and functional markers in induced pluripotent stem cell-derived retinal pigment epithelium. Stem Cells Transl Med 2:911-922.
- Severyn et al. (2016) Development of a High-Throughput Gene Expression Screen for Modulators of RAS-MAPK Signaling in a Mutant RAS Cellular Context. J Biomol screen 9:989-997.
- Neilsen et al. (2020) High-throughput identification of protein functional similarities using a gene-expression-based siRNA screen. Sci Data 7:27.
QuantiGene Plex instrumentation and equipment for high throughput and batch processing
Find instrumentation and equipment to make every step of your QuantiGene Plex 384-well workflow easy. For detailed options and recommendations for batch processing, refer to the QuantiGene Plex 384-well manual or contact your local Technical Sales Specialist.
| Workflow step | Recommended equipment | Description | |
|---|---|---|---|
| Sample incubation and target hybridization | Temperature regulated orbital shaker: MaxQ 4450 Benchtop Orbital Shakers |  | Versatile shaker ideal for incubating a small number of vessels and cell culture and many other applications. |
| Plate washes | Magnetic microplate washers | Automated magnetic microplate washer for 384-well plates. | |
| Sample incubation | Plate sealer: ALPS 50 V-Manual Heat Sealer |  | Applies consistent, secure, tight seals around individual wells, eliminating sample loss through evaporation and cross contamination between wells. |
| Sample and plate additions | Versette Automated Liquid Handler |  | Compact liquid handler featuring user-friendly programming, reliable performance, and a choice of 96- or 384-channel pipetting heads. |
| Multiplex detection | Luminex FLEXMAP 3D Instrument System |  | Most advanced and multifunctional multiplex unit for simultaneous measurement of up to 80 tests in a single reaction volume. |
QuantiGene Plex specifications
| Parameter | 96-well | 384-well | |
|---|---|---|---|
| Product specification | Sensitivity (Limit of detection) | 1,000–2,000 transcripts/assay well | 2,000–4,000 transcripts/assay well |
| Assay variation (C.V.) | ≤15% | ≤20% | |
| Linearity | ≥3 logarithmic units | ||
| Plex level | 3–80 | 3–80 | |
| Samples | Cultured cells, bacteria, whole blood, PAXgene blood or dried blood spots, fresh/frozen tissues (animal or plant), FFPE samples, purified RNA | ||
| Compatible Luminex instrumentation | MAGPIX, Luminex 200, FLEXMAP 3D, INTELLIFLEX | FLEXMAP 3D, INTELLIFLEX | |
| Assay volumes | Target probe hybridization | 100 µL | 70 µL |
| Pre-amp hybridization | 100 µL | 30 µL | |
| Amp hybridization | 100 µL | 30 µL | |
| Label probe hybridization | 100 µL | 30 µL | |
| SAPE | 100 µL | 30 µL | |
QuantiGene Plex FAQs
- What sample types are compatible with QuantiGene Plex assay?
Whole blood, fresh tissue, cultured cells, and purified RNA are validated. Other sample types including Tempus blood, bacteria, H&E stained/unstained FFPE, and blood spots should also be applicable. - What data analysis software solutions are offered for the QuantiGene Plex assay?
Analyze data using the QuantiGene cloud-based application from any computer, anywhere. For advanced analysis and visualize, data can be analyzed using the free desktop application software, Applied Biosystems Transcriptome Analysis Console (TAC) software. - Can the VorTemp™ 56 Shaking Incubator be used for hybridization?
We highly recommend the MaxQ Shaking incubator for optimal assay performance. It is possible to use the Vortemp, but 800 rpm is recommended. - What is the recommended temperature for hybridization?
The Day 1 temperature is 54°C while the Day 2 temperature is 50°C. Please use Quantigene Incubator Temperature Validation Kit (QS0517) to verify the incubator temperature. - What are the proper storage conditions for the QuantiGene Plex reagents?
When the kit arrives, please follow the instructions to store individual components. The Preamp/Amp/LP Diluent, Bead Mix, SAPE, SAPE Diluent should be stored at 4°C, while probe set mix, Proteinase K, and blocking reagent should be stored at -20°C. Others are stored at room temperature. Refer to the product insert for details. - What reagents are included in the kit?
The kit provides all necessary reagents to perform the assay except Target Probe and Bead mix which are ordered separately. See the user manual for a list of the reagents. - Can QuantiGene Plex 384-well assays be used for miRNA or DNA analysis?
No. We have options with the QuantiGene Singleplex assay for miRNA and DNA. - Should any control/housekeeping genes be used in my panel
Three or more control/housekeeping genes are recommended to normalize the gene expression results. - Can I use a heat sealer to seal the plate on day 2 of the assay
No, manual sealing using the provided seal is recommended. Heat sealing would deform the plate causing problem in washing. - How do I dilute my samples?
For cell lysate or blood lysate, please use Diluted Lysis Mix (DLM); for tissue homogenates, please use Homogenizing Solution. - How should I seal the Day 1 plate for Target Probe hybridization?
The Day 1 plate can be sealed either manually or using a heat sealer. The pressure seal for manual sealing and the heat seal AB-0757 for Thermo Fisher ALPS 50 V (AB-1443A) are provided in the kit. If a different heat sealer will be used, please consult the manufacturer for suitable sealing.
Technical guides
User manuals
User manuals for use with assay kits QP1013, QP1014, QP1015 and QP1016
QuantiGene sample processing
Application notes
Unique benefits of QuantiGene Plex Gene Expression Assay
- Increase efficiency—harmonized gene and protein multiplex solution—QuantiGene and ProcartaPlex matched panels are complementary, highly correlative and run on the same Luminex platform, seamlessly integrated for unparalleled insights
- Excellent correlation with gold standard technology—between qPCR and QuantiGene
- Works with difficult sample types—works with degraded and cross-linked RNA in FFPE tissues and directly with blood; no RNA purification required
- True multiplexing—measure up to 80 genes of interest and housekeeping genes in the same well with no cross-reactivity, helping reduce the number of samples needed
- Standardized platform—96-well plate format compatible with Luminex 200, MAGPIX, and FlexMap3D systems and 384-well with FlexMap3D system
- Simple workflow—ELISA-like workflow for target hybridization and signal amplification with no reverse transcription needed
- Large inventory of validated genes—select from over 22,000 genes to create pathway- and disease-themed panels
- Customization—if we don’t have your gene(s), we can create your custom panel within 3–4 weeks
How QuantiGene Plex Assay works
The QuantiGene Plex Assay utilizes xMAP Luminex beads for multiplexing of 3 to 80 RNA targets followed by branched DNA-based signal amplification. The assay can be performed in 96- or 384-well plates, and signal is detected using a Luminex instrument.
Video: QuantiGene Plex–How It Works animation
QuantiGene Plex Assays incorporate branched DNA technology and Luminex xMAP technology for accurate multiplex gene expression profiling. Branched DNA assays allow for the direct measurement of RNA transcripts by using signal amplification rather than target amplification.
QuantiGene Plex Gene Expression Assay workflow
The QuantiGene Plex Gene Expression Assay is a hybridization-based assay using xMAP Luminex beads and performed in 96- or 384-well plates. The assay is based on direct quantification of the RNA targets for multiplexing of 3 to 80 RNA targets and branched DNA (bDNA) signal amplification technology. On the first day the sample is lysed to release the RNAs and incubated overnight with target specific probe sets and Luminex capture beads. On the second day the signal amplification tree is built via sequential hybridization of Pre-amplifier, Amplifier, and Label Probe. Each amplification unit provides a 400x signal amplification and there are six amplification units per target RNA copy resulting in a 2,400x signal amplification per copy RNA. The signal is detected by using the fluorescent reporter molecule, phycoerythrin, on a Luminex instrument for readout and analysis.
QuantiGene Plex Assay Workflow
Step 1.Sample preparation: Samples are lysed to release and stabilize RNAs. The RNA assay works with a variety of samples such as: cultured cells, virus, bacteria, plant and animal tissues, FFPE tissues, whole blood and PAXGene blood, or purified RNA.
Step 2. Target hybridization: Overnight hybridization in the 96- or 384-well plates with the target specific probe sets panel (Capture extenders–CEs, Label Extenders–LEs, and Blocking probes).
Step 3. Signal amplification: Signal amplification is achieved using branched DNA (bDNA) technology. First, an individual Pre-Amplifier molecule hybridizes to each pair of Label Extenders. Then, multiple Amplifier molecules hybridize to each Pre-Amplifier. Finally, multiple biotinylated Label Probe oligonucleotides hybridize to each Amplifier.
Step 4. Detection: Addition of streptavidin phycoerythrin (SAPE) generates a signal that is proportional with the amount of target RNA present in the sample. The signal is read using a Luminex instrument.
Click image to enlarge
Step 5. Data Analysis: Analyze data using the Thermo Fisher Connect cloud-based QuantiGene data analysis application from any computer, anywhere. For advanced analysis and visualization, data can be analyzed using the free desktop application software, Applied Biosystems Transcriptome Analysis Console (TAC) software. TAC provides visualization and interpretation using tools such as scatter and volcano plots, hierarchical clustering, and provides link outs to publicly available annotations.
QuantiGene Plex high throughput assays
The QuantiGene Plex 384-well assay format enables multiplex gene expression analysis in a high throughput format. The assay uses target-specific probes to capture the RNA of interest and branched DNA technology to amplify fluorescent signals which are read with the FLEXMAP 3D, Luminex platform. Automation and batch processing options for this assay format increase sample throughput and decrease hands-on time while allowing for fast time to results for screening projects.
The QuantiGene Plex 384-well Assay Kit (Cat. No. QP1016) contains all the necessary reagents, buffers, and plates to perform QuantiGene Plex assays in 384-well plates. Design your target-specific QuantiGene Plex panel with our Panel Configuration Tool or contact your local Technical Sales Specialist for more information.
QuantiGene Plex 384-well example data
The QuantiGene Plex 384-well assay is designed to increase sample throughput and decrease hands-on time while allowing for fast time to results for screening projects. Below is example data that was generated using the 384-well format using the recommended equipment listed below.
Stimulation of cytokine production in HeLa cells
Cytokine production in cultured HeLa cells was stimulated using a Cell Stimulation Cocktail and monitored for various time points up to 24 hours. In this experiment, a full 80-plex panel was used for screening purposes focused on cytokine, and chemokine biomarkers.
Click image to enlarge
Figure 1. Normalized gene expression of various targets in HeLa cells at different time points post stimulation with a Cell Stimulation cocktail. Y-axis: Average normalized expression; x-axis: mRNA targets; z-axis: time points of stimulation. Only a subset of gene targets tested is being displayed. HeLa cells were cultured in a 384-well format and stimulated with a Cell Stimulation cocktail for various times up to 24h (1h, 2h, 4h, 24h). Unstimulated cells served as baseline control. At the indicated time points, cells were lysed according to the QuantiGene sample prep protocol for cell lysates using liquid handling (Thermo Fisher Platemate). After all samples from all time points were prepared, the QuantiGene Plex 384-well assay was performed. Chart illustrates the normalized gene expression results.
The normalized gene expression of various targets in HeLa cells at different time points post stimulation with a Cell Stimulation cocktail reveals the degree of up- or downregulation. The average normalized expression (y-axis) represents the level of gene induction for the mRNA targets listed on the x-axis, while the time points of stimulation are shown on the z-axis. Of note, for example IL1B, IL8 and TGFB1 show pronounced relative gene expression at various time points of stimulation. While TGFB1 relative expression seems to gradually increase over time, peak expression for IL1B is seen at later time points around 24h. Table 1 shows normalized gene expression and fold change of various targets in HeLa cells at different time points post stimulation with a Cell Stimulation cocktail.
Click image to enlarge
Table 1 and 2. Normalized gene expression and fold change of various targets in HeLa cells at different time points post stimulation with a Cell Stimulation cocktail.
See how scientists are using QuantiGene Plex 384-well in their research:
- Danahay et. al. (2015) Notch2 Is Required for Inflammatory Cytokine-Driven Goblet Cell Metaplasia in the Lung. Cell Reports 10:239-252.
- Ferrer et al. (2014) A multiplex high-throughput gene expression assay to simultaneously detect disease and functional markers in induced pluripotent stem cell-derived retinal pigment epithelium. Stem Cells Transl Med 2:911-922.
- Severyn et al. (2016) Development of a High-Throughput Gene Expression Screen for Modulators of RAS-MAPK Signaling in a Mutant RAS Cellular Context. J Biomol screen 9:989-997.
- Neilsen et al. (2020) High-throughput identification of protein functional similarities using a gene-expression-based siRNA screen. Sci Data 7:27.
QuantiGene Plex instrumentation and equipment for high throughput and batch processing
Find instrumentation and equipment to make every step of your QuantiGene Plex 384-well workflow easy. For detailed options and recommendations for batch processing, refer to the QuantiGene Plex 384-well manual or contact your local Technical Sales Specialist.
| Workflow step | Recommended equipment | Description | |
|---|---|---|---|
| Sample incubation and target hybridization | Temperature regulated orbital shaker: MaxQ 4450 Benchtop Orbital Shakers | | Versatile shaker ideal for incubating a small number of vessels and cell culture and many other applications. |
| Plate washes | Magnetic microplate washers | Automated magnetic microplate washer for 384-well plates. | |
| Sample incubation | Plate sealer: ALPS 50 V-Manual Heat Sealer | | Applies consistent, secure, tight seals around individual wells, eliminating sample loss through evaporation and cross contamination between wells. |
| Sample and plate additions | Versette Automated Liquid Handler | | Compact liquid handler featuring user-friendly programming, reliable performance, and a choice of 96- or 384-channel pipetting heads. |
| Multiplex detection | Luminex FLEXMAP 3D Instrument System | | Most advanced and multifunctional multiplex unit for simultaneous measurement of up to 80 tests in a single reaction volume. |
QuantiGene Plex specifications
| Parameter | 96-well | 384-well | |
|---|---|---|---|
| Product specification | Sensitivity (Limit of detection) | 1,000–2,000 transcripts/assay well | 2,000–4,000 transcripts/assay well |
| Assay variation (C.V.) | ≤15% | ≤20% | |
| Linearity | ≥3 logarithmic units | ||
| Plex level | 3–80 | 3–80 | |
| Samples | Cultured cells, bacteria, whole blood, PAXgene blood or dried blood spots, fresh/frozen tissues (animal or plant), FFPE samples, purified RNA | ||
| Compatible Luminex instrumentation | MAGPIX, Luminex 200, FLEXMAP 3D, INTELLIFLEX | FLEXMAP 3D, INTELLIFLEX | |
| Assay volumes | Target probe hybridization | 100 µL | 70 µL |
| Pre-amp hybridization | 100 µL | 30 µL | |
| Amp hybridization | 100 µL | 30 µL | |
| Label probe hybridization | 100 µL | 30 µL | |
| SAPE | 100 µL | 30 µL | |
QuantiGene Plex FAQs
- What sample types are compatible with QuantiGene Plex assay?
Whole blood, fresh tissue, cultured cells, and purified RNA are validated. Other sample types including Tempus blood, bacteria, H&E stained/unstained FFPE, and blood spots should also be applicable. - What data analysis software solutions are offered for the QuantiGene Plex assay?
Analyze data using the QuantiGene cloud-based application from any computer, anywhere. For advanced analysis and visualize, data can be analyzed using the free desktop application software, Applied Biosystems Transcriptome Analysis Console (TAC) software. - Can the VorTemp™ 56 Shaking Incubator be used for hybridization?
We highly recommend the MaxQ Shaking incubator for optimal assay performance. It is possible to use the Vortemp, but 800 rpm is recommended. - What is the recommended temperature for hybridization?
The Day 1 temperature is 54°C while the Day 2 temperature is 50°C. Please use Quantigene Incubator Temperature Validation Kit (QS0517) to verify the incubator temperature. - What are the proper storage conditions for the QuantiGene Plex reagents?
When the kit arrives, please follow the instructions to store individual components. The Preamp/Amp/LP Diluent, Bead Mix, SAPE, SAPE Diluent should be stored at 4°C, while probe set mix, Proteinase K, and blocking reagent should be stored at -20°C. Others are stored at room temperature. Refer to the product insert for details. - What reagents are included in the kit?
The kit provides all necessary reagents to perform the assay except Target Probe and Bead mix which are ordered separately. See the user manual for a list of the reagents. - Can QuantiGene Plex 384-well assays be used for miRNA or DNA analysis?
No. We have options with the QuantiGene Singleplex assay for miRNA and DNA. - Should any control/housekeeping genes be used in my panel
Three or more control/housekeeping genes are recommended to normalize the gene expression results. - Can I use a heat sealer to seal the plate on day 2 of the assay
No, manual sealing using the provided seal is recommended. Heat sealing would deform the plate causing problem in washing. - How do I dilute my samples?
For cell lysate or blood lysate, please use Diluted Lysis Mix (DLM); for tissue homogenates, please use Homogenizing Solution. - How should I seal the Day 1 plate for Target Probe hybridization?
The Day 1 plate can be sealed either manually or using a heat sealer. The pressure seal for manual sealing and the heat seal AB-0757 for Thermo Fisher ALPS 50 V (AB-1443A) are provided in the kit. If a different heat sealer will be used, please consult the manufacturer for suitable sealing.
Technical guides
User manuals
User manuals for use with assay kits QP1013, QP1014, QP1015 and QP1016
QuantiGene sample processing
Application notes
QuantiGene Plex Assay services
Let us test and analyze your samples to help save you time
Accelerate your research by outsourcing your gene expression profiling projects to our in-house Madison, WI scientists. Our assay service is designed to help you meet your specific project goals and timelines so you can free up your valuable resources for other discovery research.
Assay services features and benefits:
Design support—save time by accessing our bioinformatics team to customize your probe and panel design; obtain high-quality data with a fast turnaround time
Consistency—use the same customized assays in your follow-up studies that our team uses in the service
Flexibility—sample types include purified RNA, cultured cells, or tissue (whole blood, fresh or frozen tissue, or FFPE) pre-lysed in Invitrogen QuantiGene Buffer
Choice—a comprehensive list of target areas, including immunology, inflammation, cancer signalling, toxicology, neurology, cardiology, bone biology, metabolism, endocrinology, stem cell biology, apoptosis, and autophagy
Guide to designing custom panels
Learn how to design a custom Invitrogen QuantiGene Plex Assay Panel for your research needs. This application note details recommendations for housekeeping genes and how to get started.
Related products and resources
Instrumentation and software
Luminex Instruments
Explore Luminex systems: MAGPIX, Luminex 200, and FLEXMAP3D.
QuantiGene Plex software
Learn about data analysis and visualization software.
Gene expression assays
Microarrays
Microarray platform for fast, detailed transcriptome-wide expression profiling.
Real-time PCR
TaqMan Gene Expression Assays, master mixes and instrumentation.
For Research Use Only. Not for use in diagnostic procedures.