Invitrogen Novex Tris-Glycine Gels are polyacrylamide gels based on traditional Laemmli protein electrophoresis. Novex Tris-Glycine Gels offer reproducible separation of a wide range of proteins into well-resolved bands.

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Key features of the Novex Tris-Glycine Gels include:

• Excellent separation—well-resolved, straight bands
• Flexibility—use for native and denaturing protein gel electrophoresis
• High-capacity wells—WedgeWellformat wellsarewedge-shaped wells that double the well sample loading capacity and make it easier to load your sample (no specialized pipette tips required)
• Exceptional separation of cell lysates—Novex Tris-Glycine gels resolve lysate samples while other suppliers' gels smear

*Not all percentages are available in every well type

Novex Tris-Glycine Gels do not contain SDS and can be used to run your proteins in native or in denatured form. For denatured proteins, we recommend using tris-glycine SDS sample buffer and a tris-glycine SDS running buffer. For native proteins, we recommend using a tris-glycine native sample buffer and a tris-glycine native running buffer.

Novex Tris-Glycine Gels use a tris-glycine discontinuous buffer system with three ions primarily involved:

• Chloride (–), supplied by the gel buffer, serves as the leading ion because it has the highest attraction to the anode relative to other anions in the system
• Glycine (–), the primary anion provided by the running buffer, serves as the trailing ion, because it is only partially negatively charged and remains behind the more highly charged chloride ions in a charged environment
• Tris base (+) is a common ion present in both the gel and the running buffers. During electrophoresis, the gel and buffer ions in the tris-glycine system form an operating pH of 9.5 in the separating region of the gel.

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WedgeWell format wells are wedge-shaped wells that increase the sample loading capacity, allowing up to 60 µL and 100 µL of samples to be loaded in the mini and midi gel wells, respectively. WedgeWell Midi format gels that combine the higher sample throughput of wide format gels with the higher sample loading capacity of WedgeWells are now available. Key benefits include:

• Higher sample throughput—load up to 26 samples per gel without compromising on sample loading volume
• Easier sample loading—no specialized pipette tips needed
• Results you can trust—remove run-to-run variability and enhance the reproducibility of your experiments
• Load more, see more—easier detection of dilute samples and low abundance proteins
• More data in less time—reduce the need to enrich low-abundant protein samples and to concentrate dilute ones
• Reduced cost—lower cost per sample and reduced running buffer required per sample

WedgeWell format well increases the well sample loading capacity, helping prevent sample spillover and cross-contamination.

Figure 1. Increased sample volume capacity of Novex Tris-glycine midi WedgeWell format gels. (A) To compare gel spillover, increasing volumes (20–60 μL) of a fluorescent protein ladder were loaded in every other lane of a Novex WedgeWell Tris-glycine midi 20-well gel (left) or Bio-Rad 4–20% Criterio TGX Protein Gel (right). Sample spillover in Bio-Rad’s gel is seen in lanes adjacent to the 40–60 μL loading lanes.

The amount of protein you can load into a protein gel well affects the ability to detect the protein following protein gel electrophoresis or western blotting; the more you can load, the easier it is to detect. The protein load capacities of Invitrogen Novex Tris-Glycine Bis-Tris precast midi gels run in the SureLock Tandem Midi Gel Tank were compared against Bio-Rad Criterion midi gels run in a Bio-Rad Criterion Cell Midi Cell Tank using manufacturer instructions. Decreasing amounts of HEK 293 cell lysate prepared in RIPA lysis buffer (48–0.5 µg total protein) were denatured in the respective manufacturer’s sample buffer and subjected to electrophoresis using manufacturer instructions. The table below lists the samples, protein mass, and %RIPA buffer loaded in each lane.

Invitrogen Novex Tris-Glycine gels outperformed Bio-Rad gels at higher lysate loads with blots from Bio-Rad gels showing band loss and smearing at higher loads for all targets investigated.

Novex Tris-Glycine Plus, Midi, WedgeWell format gels offer increased loading capacity and excellent protein separation.

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Novex Tris-Glycine Gels are designed to deliver well-resolved, straight bands with optimal band quality as compared to other commercially available precast gels.

Novex Tris-Glycine Gel band quality

Novex Tris-Glycine Gels are designed to deliver well-resolved, straight bands with optimal band quality as compared to other commercially available precast gels.

Figure 4. Band quality with Novex Tris-Glycine gels. Protein ladders, purified proteins, and E. coli lysate were loaded on an Invitrogen Novex 4–20% Tris-Glycine Mini Gel, (A) and a 4–20% gradient gel (B). Straighter lanes with better lysate protein band sharpness and resolution are observed on gel (A). Lanes 1, 5, 10: 5 µL Thermo Scientific PageRuler Unstained Protein Ladder; lanes 2, 6, 9: 5 µL Invitrogen Mark12 Unstained Standard; lane 3: 10 µg E. coli lysate; lane 4: 6 µg BSA; lane 7: 6 µg hIgG; lane 8: 20 µg E. coli lysate.

Protein integrity

With Novex Tris-Glycine Gels, you can achieve greater protein integrity as compared to other commercially available precast gels.

Figure 6. Novex Tris-Glycine Gels offer increased protein integrity. Protein ladder, purified proteins, and E. coli lysate were loaded on a Novex 4–20% Tris-Glycine Mini Gel,(A) and a Bio-Rad TGX 4–20% gradient gel (B). The Bio-Rad TGX gel (B) displays numerous low molecular weight protein degradation products below major bands in lanes 3, 4, 7, 8. These are not seen in the Novex Tris-Glycine gel (A). Gel (A) also displays better lysate protein band sharpness and resolution than gel (B). Lanes 1, 10: 5 µL Mark12 Unstained Standard; lane 2: 10 µg E. coli lysate; lane 3: 6 µg catalase; lane 4: 6 µg carbonic anhydrase; lane 5: 6 µg lysozyme; lane 6: 6 µg hIgM ; lane 7: 6 µg BSA; lane 8: 6 µg beta-galactosidase; lane 9: 20 µg E. coli lysate.

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• Novex Tris-Glycine Wedgewell Mini Gel Protocol
• Novex Midi Gel Manual
• Mini Gel Tank Manual
• SureLock Tandem Midi Gel Tank USER GUIDE
• Protein Gel Electrophoresis Handbook
• Western Blotting Handbook

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Novex WedgeWell Tris-Glycine 겔의 특징:

• Wedge형 웰(well)—웰당 최대 60µL의 샘플을 쉽게 로딩할 수 있음
• 고성능—우수한 단백질 밴드 분리능 및 선명도
• 유효기간 연장—겔을 4°C에서 최대 12개월 보관
• 빠른 실행 조건—60분 이내에 정전압을 사용해 단백질을 신속하게 분리
• native 및 denatured 단백질 샘플 사용 가능

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권장 단백질 로딩 용적:

겔 크기

Novex™ WedgeWell Tris-Glycine 겔은 mini-gel(8cm x 8cm) 형태로 출시됩니다. mini-gel은 1.0mm 겔 단위로 이용 가능하고 Invitrogen만의 독점 WedgeWell® 형태로 되어 있어 모든 1.0mm 두께의 Tris-Glycine mini-gel을 수용할 수 있는 최대 샘플 용적을 제공합니다. Novex™ WedgeWell Tris-Glycine 겔의 형식에는 10-well, 12-well, 15-well이 있습니다. 로딩 용적에 대해서는권장 웰(well) 로딩 용적 및 샘플 로딩을 참조하십시오.

Polyacrylamide 농도

Novex WedgeWell Tris-Glycine 겔은 6%~16%의 다양한 고정 농도뿐 아니라, 4–12%, 4–20%, 8–16%, 10–20% 범위의 gradient로도 출시됩니다. 각 상자에는 겔 10개가 들어 있습니다. 단백질을 분리하는 데 사용해야 하는 겔 유형을 확인하려면, 단백질 이동 패턴을 참조하십시오.

겔 보관 및 유효기간

Novex WedgeWell Tris-Glycine 겔은 4°C에서 보관해야 하고 유효기간은 최대 1년입니다.

native 또는 denatured 단백질 실행

Novex WedgeWell Tris-Glycine 겔은 SDS를 함유하고 있지 않아 native 또는 denatured 형식의 단백질을 running하는 데 사용할 수 있습니다. denatured 단백질의 경우, Invitrogen™ Novex™ Tris-Glycine SDS Sample Buffer와 Invitrogen™Novex™ Tris-Glycine SDS Running Buffer.사용을 권장합니다. 반면에 native 단백질의 경우, Invitrogen™Novex™ Tris-Glycine Native Sample Buffer 와 Invitrogen™ Novex™ Tris-Glycine Native Running Buffer사용을 권장합니다.

겔 transfer

겔을 membrane으로 transfer할 경우에는 Invitrogen™Novex™ Tris-Glycine Transfer Buffer.사용을 권장합니다. transfer를 실시할 때에는 Invitrogen™ Mini Blot Module또는 Invitrogen™ iBlot™ 2 Dry Blotting System을 사용할 수 있습니다.