Invitrogen Novex Tris-Glycine Gels are polyacrylamide gels based on traditional Laemmli protein electrophoresis. Novex Tris-Glycine Gels offer reproducible separation of a wide range of proteins into well-resolved bands.

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Novex Tris-Glycine Gel specifications

Key features of the Novex Tris-Glycine Gels include:

  • Excellent separation—well-resolved, straight bands
  • Flexibility—use for native and denaturing protein gel electrophoresis
  • High-capacity wells—WedgeWellformat wellsarewedge-shaped wells that double the well sample loading capacity and make it easier to load your sample (no specialized pipette tips required)
  • Exceptional separation of cell lysates—Novex Tris-Glycine gels resolve lysate samples while other suppliers' gels smear
Available gel sizesMini: 8 cm x 8 cm (1.0 mm thick)
Midi: 8 cm x 13 cm (1.0 mm thick)
Available Well configurations*WedgeWell format, Midi (load up to 100 µL per well): 12+2, 20, 26 wells
WedgeWell format, Mini (load up to 60 µL per well): 10, 12, 15 wells
Midi: 12+2, 20, 26 wells
Mini: 10, 12, 15 wells
Storage conditions2–8°C
Shelf lifeUp to 12 months
Recommended sample bufferSDS-PAGE: Novex Tris-Glycine SDS Sample Buffer
Native-PAGE: Novex Tris-Glycine Native Sample Buffer
Recommended running buffersSDS-PAGE: Novex Tris-Glycine SDS Running Buffer
Native-PAGE: Novex Tris-Glycine Native Running Buffer
Recommended transfer buffersNovex Tris-Glycine Transfer Buffer
Gel chemistryTris-glycine
Available polyacrylamide concentrations6%, 8%, 10%, 12%, 14%, 16%, 4–12%, 4–20%, 8–16%, 10–20%
Separation range (denaturing)8–250 kDa
For use with (equipment) mini gelsMini Gel Tank or XCell SureLock Mini-Cell
For use with (equipment) midi gelsSureLock Tandem Midi Gel Tank, Invitrogen XCell4 SureLock Midi-Cell or Bio-Rad Criterion (with adapters only)
Mode of separationSDS-PAGE: Molecular weight
Native-PAGE: Intrinsic charge, molecular size
ApplicationsSDS-PAGE, Native-PAGE

*Not all percentages are available in every well type

Novex Tris-Glycine Gels do not contain SDS and can be used to run your proteins in native or in denatured form. For denatured proteins, we recommend using tris-glycine SDS sample buffer and a tris-glycine SDS running buffer. For native proteins, we recommend using a tris-glycine native sample buffer and a tris-glycine native running buffer.

Novex Tris-Glycine Gels use a tris-glycine discontinuous buffer system with three ions primarily involved:

  • Chloride (–), supplied by the gel buffer, serves as the leading ion because it has the highest attraction to the anode relative to other anions in the system
  • Glycine (–), the primary anion provided by the running buffer, serves as the trailing ion, because it is only partially negatively charged and remains behind the more highly charged chloride ions in a charged environment
  • Tris base (+) is a common ion present in both the gel and the running buffers. During electrophoresis, the gel and buffer ions in the tris-glycine system form an operating pH of 9.5 in the separating region of the gel.

High-capacity wells now also available in the midi gel format

WedgeWell format wells are wedge-shaped wells that increase the sample loading capacity, allowing up to 60 µL and 100 µL of samples to be loaded in the mini and midi gel wells, respectively. WedgeWell Midi format gels that combine the higher sample throughput of wide format gels with the higher sample loading capacity of WedgeWells are now available. Key benefits include:

  • Higher sample throughput—load up to 26 samples per gel without compromising on sample loading volume
  • Easier sample loading—no specialized pipette tips needed
  • Results you can trust—remove run-to-run variability and enhance the reproducibility of your experiments
  • Load more, see more—easier detection of dilute samples and low abundance proteins
  • More data in less time—reduce the need to enrich low-abundant protein samples and to concentrate dilute ones
  • Reduced cost—lower cost per sample and reduced running buffer required per sample


Recommended sample loading volumes for Novex Tris-Glycine Gels

Well sizeRecommended loading
volume (µL)
Maximum loading
volume (µL)
WedgeWell format midi gels
12 + 2 (large well)90100
12 + 2 (small well)3035
205060
263040
WedgeWell format mini gels
104060
123045
152035
171530
Midi gels
12 + 2 (large well)4560
12 + 2 (small well)1520
202530
261520
Mini gels*
91728
101525
121220
15915
17915
*1 mm thick gel


Increased sample loading capacity

WedgeWell format well increases the well sample loading capacity, helping prevent sample spillover and cross-contamination.

Sample loading capacity of Coomassie stained gels

Figure 1. Increased sample volume capacity of Novex Tris-glycine midi WedgeWell format gels. (A) To compare gel spillover, increasing volumes (20–60 μL) of a fluorescent protein ladder were loaded in every other lane of a Novex WedgeWell Tris-glycine midi 20-well gel (left) or Bio-Rad 4–20% Criterio TGX Protein Gel (right). Sample spillover in Bio-Rad’s gel is seen in lanes adjacent to the 40–60 μL loading lanes.


Comparison of electrophoresis and western blotting of cell lysates using Novex midi gels versus Bio-Rad

The amount of protein you can load into a protein gel well affects the ability to detect the protein following protein gel electrophoresis or western blotting; the more you can load, the easier it is to detect. The protein load capacities of Invitrogen Novex Tris-Glycine Bis-Tris precast midi gels run in the SureLock Tandem Midi Gel Tank were compared against Bio-Rad Criterion midi gels run in a Bio-Rad Criterion Cell Midi Cell Tank using manufacturer instructions. Decreasing amounts of HEK 293 cell lysate prepared in RIPA lysis buffer (48–0.5 µg total protein) were denatured in the respective manufacturer’s sample buffer and subjected to electrophoresis using manufacturer instructions. The table below lists the samples, protein mass, and %RIPA buffer loaded in each lane.

Invitrogen Novex Tris-Glycine gels outperformed Bio-Rad gels at higher lysate loads with blots from Bio-Rad gels showing band loss and smearing at higher loads for all targets investigated.

Lane+1123456789101112+1
SampleSample bufferiBright Protein LadderHEK293 lysateiBright Protein LadderSample buffer
Load mass (µg)NA​NA​60​50​40​30​20​10​5​2​1​0.5​NA​NA​
Load vol. (µL)5​3+25 S.B.​30​30​30​30​30​30​30​30​30​30​3+25 S.B.​5​
Conc. (µg/µL)​NA​NA​2​1.67​1.33​1​0.67​0.33​0.17​0.07​0.03​0.02​NA​NA​


Protein gel electrophoresis of cell lysates

Novex Tris-Glycine Plus, Midi, WedgeWell format gels offer increased loading capacity and excellent protein separation.

Sample loading capacity of Coomassie stained Tris-glycine midi gels

Figure 2. The protein and RIPA buffer load capacity of Novex 4–20% Tris-Glycine Plus, Midi, 12+2 well WedgeWell format gel exceeds that of Bio-Rad gels. After loading and electrophoresis, a Novex 4–20% Tris-Glycine Plus midi, 12+2 well, WedgeWell format gel, and a Bio-Rad 4–20% Criterion TGX , 12+2 well, midi gel were stained with SimplyBlue SafeStain. The Bio-Rad gel succumbs to protein and lysis buffer overload above a load of 20 µg protein, resulting in streaking and smearing. Staining near the wells indicates some protein has had difficulty entering the gel.  The Novex 4–20% Tris-Glycine Plus midi gel provides better protein band sharpness and resolution versus the Bio-Rad 4–20% TGX gel under these loading conditions.


Comparison of protein blotting and western detection of cell lysates

Western blots using Novex 4–20% Tris-Glycine Plus midi gels display sharper bands at greater protein and RIPA lysis buffer loads than Bio-Rad 4–20% TGX midi gels. The Bio-Rad blot shows streaking, bowing of bands above 24 µg protein, and bleed-over into the ladder lane for total protein analysis. With immunoblotting, the Bio-Rad blot shows band loss and smearing at higher loads for all targets.

Figure 3. Western blots using Novex 4–20% Tris-Glycine Plus midi gels display sharper bands at greater protein and RIPA lysis buffer loads than Bio-Rad 4–20% TGX midi gels. A Novex 4–20% Tris-Glycine Plus midi gel, 12+2 well, was loaded with decreasing total protein amount of HEK293 lysate, subjected to electrophoresis in a SureLock Tandem Midi Gel Tank and transferred onto a 0.45 µm PVDF membrane using the SureLock Tandem Blot Module. In parallel, a Bio-Rad 4-20% TGX midi gel, 12+2 well, was subjected to electrophoresis in a Criterion Midi Cell Tank and transferred onto a 0.45 µm PVDF membrane using the Criterion Blotter. Both membranes were analyzed for total protein using the No-Stain Protein Labeling Reagent, followed by chemiluminescent immunodetection of three targets: Vinculin, α-Tubulin, and p23. The Bio-Rad blot shows streaking, bowing of bands above 24 µg protein, and bleed-over into the ladder lane for total protein analysis. With immunoblotting, the Bio-Rad blot shows band loss and smearing at higher loads for all targets. For immunodetection: membranes were blocked for 1 hour in 1X Blocker FL Fluorescent Blocking Buffer. For chemiluminescent detection: the membranes were probed overnight with a mixture of primary antibodies diluted in blocking solution: Rabbit-anti Vinculin (1:30,000), Rat anti-α Tubulin (1:15,000), and Mouse-anti p23 (1:60,000) followed by an incubation with secondary antibodies in 1X Blocker FL: Donkey anti-Rabbit HRP (1:5,000), Donkey anti-Rat HRP (1:30,000), and Donkey anti-Mouse HRP (1:240,000) for 1 hour. Membranes were incubated for 5 minutes with SuperSignal West Dura Extended Duration Substrate and imaged for the same amount of time on an iBright Imaging System.


Novex Tris-Glycine Gels—excellent protein band resolution

Novex Tris-Glycine Gels are designed to deliver well-resolved, straight bands with optimal band quality as compared to other commercially available precast gels.

Novex Tris-Glycine Gel band quality

Novex Tris-Glycine Gels are designed to deliver well-resolved, straight bands with optimal band quality as compared to other commercially available precast gels.

High band quality is observed with Novex Tris-glycine gel compared to other commercial suppliers

Figure 4. Band quality with Novex Tris-Glycine gels. Protein ladders, purified proteins, and E. coli lysate were loaded on an Invitrogen Novex 4–20% Tris-Glycine Mini Gel, (A) and a 4–20% gradient gel (B). Straighter lanes with better lysate protein band sharpness and resolution are observed on gel (A). Lanes 1, 5, 10: 5 µL Thermo Scientific PageRuler Unstained Protein Ladder; lanes 2, 6, 9: 5 µL Invitrogen Mark12 Unstained Standard; lane 3: 10 µg E. coli lysate; lane 4: 6 µg BSA; lane 7: 6 µg hIgG; lane 8: 20 µg E. coli lysate.

Novex Tris-glycine gels deliver sharp straight bands

Figure 5. Novex Tris-Glycine Gels deliver sharp straight bands. Protein ladders and A431 cell lysate were loaded on a Novex Tris-Glycine Gel, 4–20% gradient and transferred to nitrocellulose using the Invitrogen iBlot 2 Gel Transfer Device. Lane 1: Invitrogen iBright Prestained Protein Ladder; Lane 2: Invitrogen MagicMark XP Western Protein Standard; Lanes 3–7: A431 cell lysate, 15 µg, 5 µg, 1.67 µg, 0.55 µg, 0.19 µg.

Protein integrity

With Novex Tris-Glycine Gels, you can achieve greater protein integrity as compared to other commercially available precast gels.

Novex Tris-Glycine gels offer increased protein integrity over other commercial gel suppliers

Figure 6. Novex Tris-Glycine Gels offer increased protein integrity. Protein ladder, purified proteins, and E. coli lysate were loaded on a Novex 4–20% Tris-Glycine Mini Gel,(A) and a Bio-Rad TGX 4–20% gradient gel (B). The Bio-Rad TGX gel (B) displays numerous low molecular weight protein degradation products below major bands in lanes 3, 4, 7, 8. These are not seen in the Novex Tris-Glycine gel (A). Gel (A) also displays better lysate protein band sharpness and resolution than gel (B). Lanes 1, 10: 5 µL Mark12 Unstained Standard; lane 2: 10 µg E. coli lysate; lane 3: 6 µg catalase; lane 4: 6 µg carbonic anhydrase; lane 5: 6 µg lysozyme; lane 6: 6 µg hIgM ; lane 7: 6 µg BSA; lane 8: 6 µg beta-galactosidase; lane 9: 20 µg E. coli lysate.


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novex-wedgewell-showcase

Tris-Glycine 겔의 완전히 새로운 깊이 알아보기, 60µL 로드 체적으로 더 깊이 침투

Invitrogen™ Novex™ WedgeWell™ Tris-Glycine 겔은 polyacrylamide 겔 전기영동(PAGE)을 통해 단백질을 분리하는 데 사용되는 precast mini-gel로서 Laemmli 샘플과 running buffer를 사용할 수 있습니다. 이 겔은 고품질 성능을 보장할 뿐만 아니라 다양한 단백질을 분리능이 우수한 밴드로 분리합니다. Novex WedgeWell Tris-Glycine 겔은 당사의 Invitrogen™ Mini Gel Tank에서 사용할 수 있습니다.

Novex WedgeWell Tris-Glycine 겔의 특징:

  • Wedge형 웰(well)—웰당 최대 60µL의 샘플을 쉽게 로딩할 수 있음
  • 고성능—우수한 단백질 밴드 분리능 및 선명도
  • 유효기간 연장—겔을 4°C에서 최대 12개월 보관
  • 빠른 실행 조건—60분 이내에 정전압을 사용해 단백질을 신속하게 분리
  • native 및 denatured 단백질 샘플 사용 가능

다른 회사의 겔을 사용하고 계신가요?

선택 가이드를 사용해 동등한 당사 제품을 찾아 보세요.

아래에서 최적의 Novex WedgeWell Tris-Glycine 겔을 선택하실 수 있습니다:

Number of wellsGel percentage

Novex WedgeWell Tris-Glycine 겔 이동 차트

각 특정 겔 비율(%)에 최적인 전체 분리 범위는 겔 이동 차트에서 확인하십시오.

겔 이동 차트 보기

제품 특징

wedgewell-fig1

WedgeWell Tris-Glycine 겔의 샘플 용적 수용력 증가. (왼쪽) 증가한 용량 (20 μL–60 μL) 의 형광 단백질 래더를 Invitrogen™ Novex™ WedgeWell Tris-Glycine 10-well 겔의 레인에 하나 걸러씩 로딩했습니다 . (오른쪽) 증가한 용량 (20 μL–60 μL) 의 형광 단백질 래더를 경쟁사 겔의 레인에 하나 걸러씩 로딩했습니다. (B). 겔의 경우, 50μL와 60 μL 로드 레인에 인접한 레인에서 샘플 spillover가 나타났습니다.

wedgewell-fig2

Novex WedgeWell Tris-Glycine 겔을 사용한 밴드 품질. 단백질 래더, 정제 단백질, E. coli 용해물을 4–20% gradient WedgeWell Tris-Glycine 겔(A)과 경쟁사 4–20% gradient 겔(B)에 로딩했습니다. 용해물 단백질 밴드 선명도와 분리능이 더 우수하고 더 곧은 레인이 겔 (A)에서 관찰되었습니다. 레인 1, 5, 10: 5µL Thermo Scientific™ PageRuler™ Unstained Protein Ladder, 레인 2, 6, 9: 5µL Mark12 Unstained Standard, 레인 3: 10µg E. coli 용해물(10µL의 샘플 용적), 레인 4: 6µg BSA(10µL의 샘플 용적), 레인 7: 6 µg hlgG(10µL의 샘플 용적), 레인 8: 20µg E. coli 용해물(20µL의 샘플 용적)

wedgewell-fig3

Novex WedgeWell Tris-Glycine 겔의 단백질 보전성이 더 우수함. 단백질 래더, 정제 단백질, E. coli 용해물을 4–20% gradient WedgeWell Tris-Glycine 겔(A)과 경쟁사 4–20% gradient 겔(B)에 로딩했습니다. 겔 (B)의 경우, 레인 3, 4, 7, 8의 주요 밴드 아래에서 저분자량 단백질 분리 산물이 다수 나타나 있습니다. 겔 (A)에서는 이러한 결과물이 나타나지 않습니다. 또한, 겔 (A)는 겔 (B)보다 용해물 단백질 밴드 선명도와 분리능이 우수합니다. 레인 1, 10: 5µL Mark12 Unstained Standard, 레인 2: 10µg E. coli 용해물(10µL의 샘플 용적), 레인 3: 6µg catalase(10µL의 샘플 용적), 레인 4: 6µg carbonic anhydrase(10µL의 샘플 용적), 레인 5: 6µg lysozyme(10µL의 샘플 용적), 레인 6: 6µg hIgM(10µL의 샘플 용적), 레인 7: 6µg BSA(10µL의 샘플 용적), 레인 8: 6µg beta-galactosidase(10µL의 샘플 용적), 레인 9: 20µg E. coli 용해물(20µL의 샘플 용적).

wedgewell-fig1

WedgeWell Tris-Glycine 겔의 샘플 용적 수용력 증가. (왼쪽) 증가한 용량 (20 μL–60 μL) 의 형광 단백질 래더를 Invitrogen™ Novex™ WedgeWell Tris-Glycine 10-well 겔의 레인에 하나 걸러씩 로딩했습니다 . (오른쪽) 증가한 용량 (20 μL–60 μL) 의 형광 단백질 래더를 경쟁사 겔의 레인에 하나 걸러씩 로딩했습니다. (B). 겔의 경우, 50μL와 60 μL 로드 레인에 인접한 레인에서 샘플 spillover가 나타났습니다.

wedgewell-fig2

Novex WedgeWell Tris-Glycine 겔을 사용한 밴드 품질. 단백질 래더, 정제 단백질, E. coli 용해물을 4–20% gradient WedgeWell Tris-Glycine 겔(A)과 경쟁사 4–20% gradient 겔(B)에 로딩했습니다. 용해물 단백질 밴드 선명도와 분리능이 더 우수하고 더 곧은 레인이 겔 (A)에서 관찰되었습니다. 레인 1, 5, 10: 5µL Thermo Scientific™ PageRuler™ Unstained Protein Ladder, 레인 2, 6, 9: 5µL Mark12 Unstained Standard, 레인 3: 10µg E. coli 용해물(10µL의 샘플 용적), 레인 4: 6µg BSA(10µL의 샘플 용적), 레인 7: 6 µg hlgG(10µL의 샘플 용적), 레인 8: 20µg E. coli 용해물(20µL의 샘플 용적)

wedgewell-fig3

Novex WedgeWell Tris-Glycine 겔의 단백질 보전성이 더 우수함. 단백질 래더, 정제 단백질, E. coli 용해물을 4–20% gradient WedgeWell Tris-Glycine 겔(A)과 경쟁사 4–20% gradient 겔(B)에 로딩했습니다. 겔 (B)의 경우, 레인 3, 4, 7, 8의 주요 밴드 아래에서 저분자량 단백질 분리 산물이 다수 나타나 있습니다. 겔 (A)에서는 이러한 결과물이 나타나지 않습니다. 또한, 겔 (A)는 겔 (B)보다 용해물 단백질 밴드 선명도와 분리능이 우수합니다. 레인 1, 10: 5µL Mark12 Unstained Standard, 레인 2: 10µg E. coli 용해물(10µL의 샘플 용적), 레인 3: 6µg catalase(10µL의 샘플 용적), 레인 4: 6µg carbonic anhydrase(10µL의 샘플 용적), 레인 5: 6µg lysozyme(10µL의 샘플 용적), 레인 6: 6µg hIgM(10µL의 샘플 용적), 레인 7: 6µg BSA(10µL의 샘플 용적), 레인 8: 6µg beta-galactosidase(10µL의 샘플 용적), 레인 9: 20µg E. coli 용해물(20µL의 샘플 용적).

권장 단백질 로딩 용적:

웰(Well) 유형단백질 로딩 용적최대 로딩 용적최대 단백질 로딩
10 well40 µL60 µL0.5 µg/band
12 well30 µL45 µL0.4 µg/band
15 well20 µL35 µL0.25 µg/band

겔 크기

Novex™ WedgeWell Tris-Glycine 겔은 mini-gel(8cm x 8cm) 형태로 출시됩니다. mini-gel은 1.0mm 겔 단위로 이용 가능하고 Invitrogen만의 독점 WedgeWell® 형태로 되어 있어 모든 1.0mm 두께의 Tris-Glycine mini-gel을 수용할 수 있는 최대 샘플 용적을 제공합니다. Novex™ WedgeWell Tris-Glycine 겔의 형식에는 10-well, 12-well, 15-well이 있습니다. 로딩 용적에 대해서는권장 웰(well) 로딩 용적 및 샘플 로딩을 참조하십시오.

Polyacrylamide 농도

Novex WedgeWell Tris-Glycine 겔은 6%~16%의 다양한 고정 농도뿐 아니라, 4–12%, 4–20%, 8–16%, 10–20% 범위의 gradient로도 출시됩니다. 각 상자에는 겔 10개가 들어 있습니다. 단백질을 분리하는 데 사용해야 하는 겔 유형을 확인하려면, 단백질 이동 패턴을 참조하십시오.

겔 보관 및 유효기간

Novex WedgeWell Tris-Glycine 겔은 4°C에서 보관해야 하고 유효기간은 최대 1년입니다.

native 또는 denatured 단백질 실행

Novex WedgeWell Tris-Glycine 겔은 SDS를 함유하고 있지 않아 native 또는 denatured 형식의 단백질을 running하는 데 사용할 수 있습니다. denatured 단백질의 경우, Invitrogen™ Novex™ Tris-Glycine SDS Sample Buffer와 Invitrogen™Novex™ Tris-Glycine SDS Running Buffer.사용을 권장합니다. 반면에 native 단백질의 경우, Invitrogen™Novex™ Tris-Glycine Native Sample Buffer 와 Invitrogen™ Novex™ Tris-Glycine Native Running Buffer사용을 권장합니다.

겔 transfer

겔을 membrane으로 transfer할 경우에는 Invitrogen™Novex™ Tris-Glycine Transfer Buffer.사용을 권장합니다. transfer를 실시할 때에는 Invitrogen™ Mini Blot Module또는 Invitrogen™ iBlot™ 2 Dry Blotting System을 사용할 수 있습니다.

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