Number of Transfections 5 nmol of siRNA
Plate Type (Transfection Vol.)	10 nM Final siRNA Concentration	100 nM Final siRNA Concentration
6 well (2.5 ml)	200	20
12 well (1 ml)	500	50
24 well (0.5 ml)	100	10
96 well (0.1 ml)	5000	50

	Number of Transfections 20 nmol of siRNA
Plate Type (Transfection Vol.)	10 nM Final siRNA Concentration	100 nM Final siRNA Concentration
6 well (2.5 ml)	800	80
12 well (1 ml)	2000	200
24 well (0.5 ml)	4000	400
96 well (0.1 ml)	20,000	2000

	Number of Transfections 40 nmol of siRNA
Plate Type (Transfection Vol.)	10 nM Final siRNA Concentration	100 nM Final siRNA Concentration
6 well (2.5 ml)	1600	160
12 well (1 ml)	4000	400
24 well (0.5 ml)	8000	800
96 well (0.1 ml)	40,000	4000