Invitrogen

CD45.2 Monoclonal Antibody (104), eFluor™ 506, eBioscience™

CD45.2 Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 45

CD45.2 Antibody (69-0454-82) in Flow

Staining of SJL (blue histogram) and BALB/c (purple histogram) splenocytes with 0.125 µg of Anti-Mouse CD45-2 eFluor® 506. Cells in the lymphocyte gate were used for analysis.

CD45.2 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

69-0454-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Flow Cytometry (Flow)

0.25 µg/test

View 34 publications 34 publications

Product Specifications

Species Reactivity

Mouse

Published species

Fish, Mouse

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), eFluor™ 506, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

104

Conjugate

eFluor™ 506 eFluor™ 506 eFluor™ 506

Excitation/Emission Max

419/508 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2637105

Product Specific Information

Description: The 104 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.2-expressing mouse strains. The strains that express CD45.2 include the most commonly used mouse strains C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A and AKR. CD45.2 is expressed by all leukocytes in these strains.

Applications Reported: This 104 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 104 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 506 can be excited with the violet laser line (405 nm) and emits at 506 nm. We recommend using a 510/20 band pass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 405 nm; Emission: 506 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD45.2 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45.2 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45.2 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45.2 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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69-0454-82 was used in Flow cytometry/Cell sorting to reveal an important role for CD82 in restraining phagocyte infiltration and mediating their differentiation in response to stimulatory cues.

Fri Jul 15 00:00:00 EDT 2022

Smc3 dosage regulates B cell transit through germinal centers and restricts their malignant transformation.

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Rivas MA,Meydan C,Chin CR,Challman MF,Kim D,Bhinder B,Kloetgen A,Viny AD,Teater MR,McNally DR,Doane AS,Béguelin W,Fernández MTC,Shen H,Wang X,Levine RL,Chen Z,Tsirigos A,Elemento O,Mason CE,Melnick AM

Published figure using CD45.2 monoclonal antibody (Product # 69-0454-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Niche-specific MHC II and PD-L1 regulate CD4+CD8αα+ intraepithelial lymphocyte differentiation.

The Journal of experimental medicine

Moon S,Park Y,Hyeon S,Kim YM,Kim JH,Kim H,Park S,Lee KJ,Koo BK,Ha SJ,Lee SW

Published figure using CD45.2 monoclonal antibody (Product # 69-0454-82) in Flow Cytometry

Mon Apr 05 00:00:00 EDT 2021

Aggravation of hepatic ischemia‑reperfusion injury with increased inflammatory cell infiltration is associated with the TGF‑β/Smad3 signaling pathway.

Molecular medicine reports

Li H,Shen X,Tong Y,Ji T,Feng Y,Tang Y,Mai R,Ye J,Que T,Luo X

Published figure using CD45.2 monoclonal antibody (Product # 69-0454-82) in Immunohistochemistry

Sun Aug 01 00:00:00 EDT 2021

Brd4 modulates diet-induced obesity via PPARγ-dependent Gdf3 expression in adipose tissue macrophages.

JCI insight

Hu X,Dong X,Li G,Chen Y,Chen J,He X,Sun H,Kim DH,Kemper JK,Chen LF

Published figure using CD45.2 monoclonal antibody (Product # 69-0454-82) in Flow Cytometry

Thu Apr 08 00:00:00 EDT 2021

Differentiation of fetal hematopoietic stem cells requires ARID4B to restrict autocrine KITLG/KIT-Src signaling.

Cell reports

Young IC,Wu B,Andricovich J,Chuang ST,Li R,Tzatsos A,Wu RC,Wu MY

Published figure using CD45.2 monoclonal antibody (Product # 69-0454-82) in Flow Cytometry

Tue Nov 23 00:00:00 EST 2021

Erythritol Ameliorates Small Intestinal Inflammation Induced by High-Fat Diets and Improves Glucose Tolerance.

International journal of molecular sciences

Kawano R,Okamura T,Hashimoto Y,Majima S,Senmaru T,Ushigome E,Asano M,Yamazaki M,Takakuwa H,Sasano R,Nakanishi N,Hamaguchi M,Fukui M

Published figure using CD45.2 monoclonal antibody (Product # 69-0454-82) in Flow Cytometry

Mon May 24 00:00:00 EDT 2021

Expansion of an Unusual Virtual Memory CD8⁺ Subpopulation Bearing Vα3.2 TCR in Themis-Deficient Mice.

Frontiers in immunology

Prasad M,Wojciech L,Brzostek J,Hu J,Chua YL,Tung DWH,Yap J,Rybakin V,Gascoigne NRJ

Published figure using CD45.2 monoclonal antibody (Product # 69-0454-82) in Flow Cytometry

Thu Sep 23 00:00:00 EDT 2021

The subunits of IL-12, originating from two distinct cells, can functionally synergize to protect against pathogen dissemination in vivo.

Cell reports

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Published figure using CD45.2 monoclonal antibody (Product # 69-0454-82) in Flow Cytometry

Tue Oct 12 00:00:00 EDT 2021

Ddb1 Is Essential for the Expansion of CD4⁺ Helper T Cells by Regulating Cell Cycle Progression and Cell Death.

Frontiers in immunology

Yang L,Chen W,Li L,Xiao Y,Fan S,Zhang Q,Xia T,Li M,Hong Y,Zhao T,Li Q,Liu WH,Xiao N

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Mon Dec 20 00:00:00 EST 2021

Abraxane-induced bone marrow CD11b⁺ myeloid cell depletion in tumor-bearing mice is visualized by μPET-CT with ⁶⁴Cu-labeled anti-CD11b and prevented by anti-CSF-1.

Theranostics

Cao Q,Huang Q,Wang YA,Li C

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Fri Jul 23 00:00:00 EDT 2021

A distal enhancer at risk locus 11q13.5 promotes suppression of colitis by T_reg cells.

Nature

Nasrallah R,Imianowski CJ,Bossini-Castillo L,Grant FM,Dogan M,Placek L,Kozhaya L,Kuo P,Sadiyah F,Whiteside SK,Mumbach MR,Glinos D,Vardaka P,Whyte CE,Lozano T,Fujita T,Fujii H,Liston A,Andrews S,Cozzani A,Yang J,Mitra S,Lugli E,Chang HY,Unutmaz D,Trynka G,Roychoudhuri R

69-0454 was used in Flow cytometry/Cell sorting to provide a mechanistic basis for association of the 11q13.5 risk locus with immune-mediated diseases and identify GARP as a potential target in their therapy.

Wed Jul 01 00:00:00 EDT 2020

Deregulated Notch and Wnt signaling activates early-stage myeloid regeneration pathways in leukemia.

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Mon Mar 02 00:00:00 EST 2020

Proenkephalin⁺ regulatory T cells expanded by ultraviolet B exposure maintain skin homeostasis with a healing function.

Proceedings of the National Academy of Sciences of the United States of America

Shime H,Odanaka M,Tsuiji M,Matoba T,Imai M,Yasumizu Y,Uraki R,Minohara K,Watanabe M,Bonito AJ,Fukuyama H,Ohkura N,Sakaguchi S,Morita A,Yamazaki S

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Tue Aug 25 00:00:00 EDT 2020

Interleukin 35 Delays Hindlimb Ischemia-Induced Angiogenesis Through Regulating ROS-Extracellular Matrix but Spares Later Regenerative Angiogenesis.

Frontiers in immunology

Fu H,Sun Y,Shao Y,Saredy J,Cueto R,Liu L,Drummer C,Johnson C,Xu K,Lu Y,Li X,Meng S,Xue ER,Tan J,Jhala NC,Yu D,Zhou Y,Bayless KJ,Yu J,Rogers TJ,Hu W,Snyder NW,Sun J,Qin X,Jiang X,Wang H,Yang X

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Fri Jul 09 00:00:00 EDT 2021

The frequency of follicular T helper cells differs in acute and chronic neuroinflammation.

Scientific reports

Baniahmad A,Birkner K,Görg J,Loos J,Zipp F,Wasser B,Bittner S

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Tue Nov 24 00:00:00 EST 2020

Long-term ex vivo haematopoietic-stem-cell expansion allows nonconditioned transplantation.

Nature

Wilkinson AC,Ishida R,Kikuchi M,Sudo K,Morita M,Crisostomo RV,Yamamoto R,Loh KM,Nakamura Y,Watanabe M,Nakauchi H,Yamazaki S

Published figure using CD45.2 monoclonal antibody (Product # 69-0454-82) in Flow Cytometry

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The H3K4 methyltransferase Setd1b is essential for hematopoietic stem and progenitor cell homeostasis in mice.

eLife

Schmidt K,Zhang Q,Tasdogan A,Petzold A,Dahl A,Arneth BM,Slany R,Fehling HJ,Kranz A,Stewart AF,Anastassiadis K

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Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus.

eLife

Moretti FA,Klapproth S,Ruppert R,Margraf A,Weber J,Pick R,Scheiermann C,Sperandio M,Fässler R,Moser M

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Elimination of intravascular thrombi prevents early mortality and reduces gliosis in hyper-inflammatory experimental cerebral malaria.

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Myeloid progenitor cluster formation drives emergency and leukaemic myelopoiesis.

Nature

Hérault A,Binnewies M,Leong S,Calero-Nieto FJ,Zhang SY,Kang YA,Wang X,Pietras EM,Chu SH,Barry-Holson K,Armstrong S,Göttgens B,Passegué E

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Sphingosine-1-Phosphate Prevents Egress of Hematopoietic Stem Cells From Liver to Reduce Fibrosis.

Gastroenterology

King A,Houlihan DD,Kavanagh D,Haldar D,Luu N,Owen A,Suresh S,Than NN,Reynolds G,Penny J,Sumption H,Ramachandran P,Henderson NC,Kalia N,Frampton J,Adams DH,Newsome PN

69-0454 was used in Flow cytometry/Cell sorting to investigate the role of sphingosine-1-phosphate in the response of hematopoietic stem cells to liver fibrosis in mice.

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Egr2 and 3 control adaptive immune responses by temporally uncoupling expansion from T cell differentiation.

The Journal of experimental medicine

Miao T,Symonds ALJ,Singh R,Symonds JD,Ogbe A,Omodho B,Zhu B,Li S,Wang P

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Mon Jun 05 00:00:00 EDT 2017

Recipient bone marrow assimilates the myeloid/lymphoid reconstitution of distinct fetal hematopoietic stem cells.

Oncotarget

Guo XL,Chu L,Ke F,Mu LL,Li Z,Cai JJ,Li HF,Hong DL

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Tue Dec 12 00:00:00 EST 2017

Pneumocystis-Driven Inducible Bronchus-Associated Lymphoid Tissue Formation Requires Th2 and Th17 Immunity.

Cell reports

Eddens T,Elsegeiny W,Garcia-Hernadez ML,Castillo P,Trevejo-Nunez G,Serody K,Campfield BT,Khader SA,Chen K,Rangel-Moreno J,Kolls JK

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Intratumoral modulation of the inducible co-stimulator ICOS by recombinant oncolytic virus promotes systemic anti-tumour immunity.

Nature communications

Zamarin D,Holmgaard RB,Ricca J,Plitt T,Palese P,Sharma P,Merghoub T,Wolchok JD,Allison JP

Published figure using CD45.2 monoclonal antibody (Product # 69-0454-82) in Flow Cytometry

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Host-Protozoan Interactions Protect from Mucosal Infections through Activation of the Inflammasome.

Cell

Chudnovskiy A,Mortha A,Kana V,Kennard A,Ramirez JD,Rahman A,Remark R,Mogno I,Ng R,Gnjatic S,Amir ED,Solovyov A,Greenbaum B,Clemente J,Faith J,Belkaid Y,Grigg ME,Merad M

Published figure using CD45.2 monoclonal antibody (Product # 69-0454-82) in Flow Cytometry

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The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity.

Cell

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Published figure using CD45.2 monoclonal antibody (Product # 69-0454-82) in Flow Cytometry

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LTβR controls thymic portal endothelial cells for haematopoietic progenitor cell homing and T-cell regeneration.

Nature communications

Shi Y,Wu W,Chai Q,Li Q,Hou Y,Xia H,Ren B,Xu H,Guo X,Jin C,Lv M,Wang Z,Fu YX,Zhu M

Published figure using CD45.2 monoclonal antibody (Product # 69-0454-82) in Immunofluorescence

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Atypical chemokine receptor 1 deficiency reduces atherogenesis in ApoE-knockout mice.

Cardiovascular research

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Axitinib increases the infiltration of immune cells and reduces the suppressive capacity of monocytic MDSCs in an intracranial mouse melanoma model.

Oncoimmunology

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Published figure using CD45.2 monoclonal antibody (Product # 69-0454-82) in Flow Cytometry

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WASH is required for the differentiation commitment of hematopoietic stem cells in a c-Myc-dependent manner.

The Journal of experimental medicine

Xia P,Wang S,Huang G,Zhu P,Li M,Ye B,Du Y,Fan Z

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Mon Sep 22 00:00:00 EDT 2014

Leukocyte population dynamics and detection of IL-9 as a major cytokine at the mouse fetal-maternal interface.

PloS one

Habbeddine M,Verbeke P,Karaz S,Bobé P,Kanellopoulos-Langevin C

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Wed Jun 10 00:00:00 EDT 2015

Mutual expression of the transcription factors Runx3 and ThPOK regulates intestinal CD4⁺ T cell immunity.

Nature immunology

Reis BS,Rogoz A,Costa-Pinto FA,Taniuchi I,Mucida D

Published figure using CD45.2 monoclonal antibody (Product # 69-0454-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2013

miR-126 and miR-126* repress recruitment of mesenchymal stem cells and inflammatory monocytes to inhibit breast cancer metastasis.

Nature cell biology

Zhang Y,Yang P,Sun T,Li D,Xu X,Rui Y,Li C,Chong M,Ibrahim T,Mercatali L,Amadori D,Lu X,Xie D,Li QJ,Wang XF

Published figure using CD45.2 monoclonal antibody (Product # 69-0454-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2013

Arrayed lentiviral barcoding for quantification analysis of hematopoietic dynamics.

Stem cells (Dayton, Ohio)

Grosselin J,Sii-Felice K,Payen E,Chretien S,Tronik-Le Roux D,Leboulch P

Published figure using CD45.2 monoclonal antibody (Product # 69-0454-82) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2013

The cardioprotective protein apolipoprotein A1 promotes potent anti-tumorigenic effects.

The Journal of biological chemistry

Zamanian-Daryoush M,Lindner D,Tallant TC,Wang Z,Buffa J,Klipfell E,Parker Y,Hatala D,Parsons-Wingerter P,Rayman P,Yusufishaq MSS,Fisher EA,Smith JD,Finke J,DiDonato JA,Hazen SL

Published figure using CD45.2 monoclonal antibody (Product # 69-0454-82) in Flow Cytometry

Fri Jul 19 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: CD45; CD45 antigen; GP180; L-CA; LCA; Leukocyte common antigen; Ly-5; LY5; Lymphocyte antigen 5; lymphocyte common antigen; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein

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Gene Aliases: B220; Cd45; CD45R; L-CA; loc; Ly-5; Lyt-4; Ptprc; T200

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UniProt ID: (Mouse) P06800

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Entrez Gene ID: (Mouse) 19264

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Function(s)

phosphoprotein phosphatase activity protein tyrosine phosphatase activity protein binding heparin binding hydrolase activity phosphatase activity protein kinase binding heparan sulfate proteoglycan binding protein phosphatase protein modifying enzyme

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD45.2 Monoclonal Antibody (104), eFluor™ 506, eBioscience™

CD45.2 Antibody (69-0454-82) in Flow

Product Details

69-0454-82

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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