Invitrogen

CD45.2 Monoclonal Antibody (104), Super Bright™ 780, eBioscience™

CD45.2 Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 14

CD45.2 Antibody (78-0454-82) in Flow

BALB/c mouse splenocytes were stained with CD3e Monoclonal Antibody, APC (Product # 17-0031-82) and 0.5 µg of Mouse IgG2a kappa Isotype Control, Super Bright 780 (Product # 78-4724-82) (left) or 0.5 µg of CD45.2 Monoclonal Antibody, Super Bright 780 (right). Total cells were used for analysis.

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Product Details

78-0454-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

1.0 µg/test

View 14 publications 14 publications

Product Specifications

Species Reactivity

Mouse

Published species

Not Applicable

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), Super Bright™ 780, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

104

Conjugate

Super Bright™ 780 Super Bright™ 780 Super Bright™ 780

Excitation/Emission Max

413/780 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2802469

Product Specific Information

Description: The 104 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.2-expressing mouse strains. The strains that express CD45.2 include the most commonly used mouse strains C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A and AKR. CD45.2 is expressed by all leukocytes in these strains.

Applications Reported: This 104 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 104 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 780 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 780 nm. We recommend using a 780/60 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

In some experiments, we have observed that compensation values for Super Bright 780-conjugated antibodies are higher in the violet 450/50 channel when using UltraComp eBeads microspheres (Product # 01-2222-42) as compared to single-color stained cells. In such circumstances, we would recommend setting compensation with cells. We have also observed this in some experiments using AbC Total Antibody Compensation beads (Product # A10497).

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 780 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD45.2 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45.2 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45.2 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45.2 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Smc3 dosage regulates B cell transit through germinal centers and restricts their malignant transformation.

Nature immunology

Rivas MA,Meydan C,Chin CR,Challman MF,Kim D,Bhinder B,Kloetgen A,Viny AD,Teater MR,McNally DR,Doane AS,Béguelin W,Fernández MTC,Shen H,Wang X,Levine RL,Chen Z,Tsirigos A,Elemento O,Mason CE,Melnick AM

Published figure using CD45.2 monoclonal antibody (Product # 78-0454-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Niche-specific MHC II and PD-L1 regulate CD4+CD8αα+ intraepithelial lymphocyte differentiation.

The Journal of experimental medicine

Moon S,Park Y,Hyeon S,Kim YM,Kim JH,Kim H,Park S,Lee KJ,Koo BK,Ha SJ,Lee SW

Published figure using CD45.2 monoclonal antibody (Product # 78-0454-82) in Flow Cytometry

Mon Apr 05 00:00:00 EDT 2021

Aggravation of hepatic ischemia‑reperfusion injury with increased inflammatory cell infiltration is associated with the TGF‑β/Smad3 signaling pathway.

Molecular medicine reports

Li H,Shen X,Tong Y,Ji T,Feng Y,Tang Y,Mai R,Ye J,Que T,Luo X

Published figure using CD45.2 monoclonal antibody (Product # 78-0454-82) in Immunohistochemistry

Sun Aug 01 00:00:00 EDT 2021

Brd4 modulates diet-induced obesity via PPARγ-dependent Gdf3 expression in adipose tissue macrophages.

JCI insight

Hu X,Dong X,Li G,Chen Y,Chen J,He X,Sun H,Kim DH,Kemper JK,Chen LF

Published figure using CD45.2 monoclonal antibody (Product # 78-0454-82) in Flow Cytometry

Thu Apr 08 00:00:00 EDT 2021

Differentiation of fetal hematopoietic stem cells requires ARID4B to restrict autocrine KITLG/KIT-Src signaling.

Cell reports

Young IC,Wu B,Andricovich J,Chuang ST,Li R,Tzatsos A,Wu RC,Wu MY

Published figure using CD45.2 monoclonal antibody (Product # 78-0454-82) in Flow Cytometry

Tue Nov 23 00:00:00 EST 2021

Erythritol Ameliorates Small Intestinal Inflammation Induced by High-Fat Diets and Improves Glucose Tolerance.

International journal of molecular sciences

Kawano R,Okamura T,Hashimoto Y,Majima S,Senmaru T,Ushigome E,Asano M,Yamazaki M,Takakuwa H,Sasano R,Nakanishi N,Hamaguchi M,Fukui M

Published figure using CD45.2 monoclonal antibody (Product # 78-0454-82) in Flow Cytometry

Mon May 24 00:00:00 EDT 2021

Expansion of an Unusual Virtual Memory CD8⁺ Subpopulation Bearing Vα3.2 TCR in Themis-Deficient Mice.

Frontiers in immunology

Prasad M,Wojciech L,Brzostek J,Hu J,Chua YL,Tung DWH,Yap J,Rybakin V,Gascoigne NRJ

Published figure using CD45.2 monoclonal antibody (Product # 78-0454-82) in Flow Cytometry

Thu Sep 23 00:00:00 EDT 2021

The subunits of IL-12, originating from two distinct cells, can functionally synergize to protect against pathogen dissemination in vivo.

Cell reports

Gerber AN,Abdi K,Singh NJ

Published figure using CD45.2 monoclonal antibody (Product # 78-0454-82) in Flow Cytometry

Tue Oct 12 00:00:00 EDT 2021

Ddb1 Is Essential for the Expansion of CD4⁺ Helper T Cells by Regulating Cell Cycle Progression and Cell Death.

Frontiers in immunology

Yang L,Chen W,Li L,Xiao Y,Fan S,Zhang Q,Xia T,Li M,Hong Y,Zhao T,Li Q,Liu WH,Xiao N

Published figure using CD45.2 monoclonal antibody (Product # 78-0454-82) in Flow Cytometry

Mon Dec 20 00:00:00 EST 2021

Abraxane-induced bone marrow CD11b⁺ myeloid cell depletion in tumor-bearing mice is visualized by μPET-CT with ⁶⁴Cu-labeled anti-CD11b and prevented by anti-CSF-1.

Theranostics

Cao Q,Huang Q,Wang YA,Li C

Published figure using CD45.2 monoclonal antibody (Product # 78-0454-82) in Flow Cytometry

Fri Jul 23 00:00:00 EDT 2021

Deregulated Notch and Wnt signaling activates early-stage myeloid regeneration pathways in leukemia.

The Journal of experimental medicine

Kang YA,Pietras EM,Passegué E

Published figure using CD45.2 monoclonal antibody (Product # 78-0454-82) in Flow Cytometry

Mon Mar 02 00:00:00 EST 2020

Proenkephalin⁺ regulatory T cells expanded by ultraviolet B exposure maintain skin homeostasis with a healing function.

Proceedings of the National Academy of Sciences of the United States of America

Shime H,Odanaka M,Tsuiji M,Matoba T,Imai M,Yasumizu Y,Uraki R,Minohara K,Watanabe M,Bonito AJ,Fukuyama H,Ohkura N,Sakaguchi S,Morita A,Yamazaki S

Published figure using CD45.2 monoclonal antibody (Product # 78-0454-82) in Flow Cytometry

Tue Aug 25 00:00:00 EDT 2020

Interleukin 35 Delays Hindlimb Ischemia-Induced Angiogenesis Through Regulating ROS-Extracellular Matrix but Spares Later Regenerative Angiogenesis.

Frontiers in immunology

Fu H,Sun Y,Shao Y,Saredy J,Cueto R,Liu L,Drummer C,Johnson C,Xu K,Lu Y,Li X,Meng S,Xue ER,Tan J,Jhala NC,Yu D,Zhou Y,Bayless KJ,Yu J,Rogers TJ,Hu W,Snyder NW,Sun J,Qin X,Jiang X,Wang H,Yang X

Published figure using CD45.2 monoclonal antibody (Product # 78-0454-82) in Flow Cytometry

Fri Jul 09 00:00:00 EDT 2021

The frequency of follicular T helper cells differs in acute and chronic neuroinflammation.

Scientific reports

Baniahmad A,Birkner K,Görg J,Loos J,Zipp F,Wasser B,Bittner S

Published figure using CD45.2 monoclonal antibody (Product # 78-0454-82) in Flow Cytometry

Tue Nov 24 00:00:00 EST 2020

Long-term ex vivo haematopoietic-stem-cell expansion allows nonconditioned transplantation.

Nature

Wilkinson AC,Ishida R,Kikuchi M,Sudo K,Morita M,Crisostomo RV,Yamamoto R,Loh KM,Nakamura Y,Watanabe M,Nakauchi H,Yamazaki S

Published figure using CD45.2 monoclonal antibody (Product # 78-0454-82) in Flow Cytometry

Mon Jul 01 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: CD45; CD45 antigen; GP180; L-CA; LCA; Leukocyte common antigen; Ly-5; LY5; Lymphocyte antigen 5; lymphocyte common antigen; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein

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Gene Aliases: B220; Cd45; CD45R; L-CA; loc; Ly-5; Lyt-4; Ptprc; T200

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UniProt ID: (Mouse) P06800

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Entrez Gene ID: (Mouse) 19264

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Function(s)

phosphoprotein phosphatase activity protein tyrosine phosphatase activity protein binding heparin binding hydrolase activity phosphatase activity protein kinase binding heparan sulfate proteoglycan binding protein phosphatase protein modifying enzyme

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD45.2 Monoclonal Antibody (104), Super Bright™ 780, eBioscience™

CD45.2 Antibody (78-0454-82) in Flow

Product Details

78-0454-82

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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