Invitrogen

CD4 Monoclonal Antibody (OKT4 (OKT-4)), Alexa Fluor™ 700, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

28 Published Figures

34 References

View all (606) CD4 antibodies

Datasheet

Protocols

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Datasheet

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CD4 Antibody (56-0048-82) in Flow

Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD3 FITC, CD8 PE and CD4 Alexa Fluor 700. As expected based on known relative expression patterns, CD4 clone OKT4 (OKT-4) stains a subset of lymphocytes (pink) and monocytes (orange), but not granulocytes (blue).

CD4 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

CD4 Antibody in Immunocytochemistry (ICC/IF)

CD4 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CD4 Antibody — Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD3 FITC, CD8 PE and CD4 Alexa Fluor 700. As expected based on known relative expression patterns, CD4 clone OKT4 (OKT-4) stains a subset of lymphocytes (pink) and monocytes (orange), but not granulocytes (blue). {RE}

Product Details

56-0048-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.25 µg/test

View 32 publications 32 publications

Product Specifications

Species Reactivity

Human

Published species

Cynomolgus monkey, Hamster, Human, Mouse, Rhesus monkey

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), Alexa Fluor™ 700, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

OKT4 (OKT-4)

Conjugate

Alexa Fluor™ 700 Alexa Fluor™ 700 Alexa Fluor™ 700

Excitation/Emission Max

696/719 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_657741

Product Specific Information

Description: The OKT4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface glycoprotein expressed by the majority of thymocytes, a subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). The OKT4 antibody recognizes a different epitope than the RPA-T4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.

Applications Reported:This OKT4 (OKT-4) antibody has been reported for use in flow cytometric analysis.

Applications Tested: Has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

The Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Distinct SARS-CoV-2 specific NLRP3 and IL-1β responses in T cells of aging patients during acute COVID-19 infection.

Frontiers in immunology

Mahalingam SS,Jayaraman S,Arunkumar A,Dudley HM,Anthony DD,Shive CL,Jacobson JM,Pandiyan P

56-0048-82 was used in Flow Cytometry to reveal SARS-CoV-2-specific CD4+ and CD8+ T-cell intrinsic cytokine alterations in the individuals older than 61 and may provide new insights into dysregulated COVID-directed immune responses in the elderly.

Thu Oct 12 00:00:00 EDT 2023

Distinct SARS-CoV-2 specific NLRP3 and IL-1β responses in T cells of aging patients during acute COVID-19 infection.

Frontiers in immunology

Mahalingam SS,Jayaraman S,Arunkumar A,Dudley HM,Anthony DD,Shive CL,Jacobson JM,Pandiyan P

Thu Oct 12 00:00:00 EDT 2023

Estrogen Protects against Renal Ischemia-Reperfusion Injury by Regulating Th17/Treg Cell Immune Balance.

Disease markers

Zhang Y,Chang Y,Han Z,Ma K,Zeng X,Li L

Published figure using CD4 monoclonal antibody (Product # 56-0048-82) in Flow Cytometry

Tue Oct 18 00:00:00 EDT 2022

Plasma Extracellular Vesicle Subtypes May be Useful as Potential Biomarkers of Immune Activation in People With HIV.

Pathogens & immunity

Bazié WW,Boucher J,Vitry J,Goyer B,Routy JP,Tremblay C,Trottier S,Jenabian MA,Provost P,Alary M,Gilbert C

Published figure using CD4 monoclonal antibody (Product # 56-0048-82) in Flow Cytometry

Sat Apr 23 00:00:00 EDT 2022

Targeting Phosphatidylserine Enhances the Anti-tumor Response to Tumor-Directed Radiation Therapy in a Preclinical Model of Melanoma.

Cell reports

Budhu S,Giese R,Gupta A,Fitzgerald K,Zappasodi R,Schad S,Hirschhorn D,Campesato LF,De Henau O,Gigoux M,Liu C,Mazo G,Deng L,Barker CA,Wolchok JD,Merghoub T

Published figure using CD4 monoclonal antibody (Product # 56-0048-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Small-molecule antagonist of VLA-4 (GW559090) attenuated neuro-inflammation by targeting Th17 cell trafficking across the blood-retinal barrier in experimental autoimmune uveitis.

Journal of neuroinflammation

Chen YH,Eskandarpour M,Zhang X,Galatowicz G,Greenwood J,Lightman S,Calder V

Published figure using CD4 monoclonal antibody (Product # 56-0048-82) in Flow Cytometry

Thu Feb 18 00:00:00 EST 2021

A Cross-Reactive Small Protein Binding Domain Provides a Model to Study Off-Tumor CAR-T Cell Toxicity.

Molecular therapy oncolytics

Hammill JA,Kwiecien JM,Dvorkin-Gheva A,Lau VWC,Baker C,Wu Y,Bezverbnaya K,Aarts C,Heslen CW,Denisova GF,Derocher H,Milne K,Nelson BH,Bramson JL

Published figure using CD4 monoclonal antibody (Product # 56-0048-82) in Flow Cytometry

Fri Jun 26 00:00:00 EDT 2020

The Potential of Nanobody-Targeted Photodynamic Therapy to Trigger Immune Responses.

Cancers

Beltrán Hernández I,Angelier ML,Del Buono D'Ondes T,Di Maggio A,Yu Y,Oliveira S

Published figure using CD4 monoclonal antibody (Product # 56-0048-82) in Flow Cytometry

Wed Apr 15 00:00:00 EDT 2020

Neutralizing Antibodies Protect against Oral Transmission of Lymphocryptovirus.

Cell reports. Medicine

Singh S,Homad LJ,Akins NR,Stoffers CM,Lackhar S,Malhi H,Wan YH,Rawlings DJ,McGuire AT

56-0048-82 was used in Flow Cytometry to indicate that a vaccine capable of eliciting adequate titers of neutralizing antibodies targeting the AMMO1 epitope may protect against EBV acquisition and are therefore highly relevant to the design of an effective EBV vaccine.

Tue Jun 23 00:00:00 EDT 2020

Using Serum Metabolomics to Predict Development of Anti-drug Antibodies in Multiple Sclerosis Patients Treated With IFNβ.

Frontiers in immunology

Waddington KE,Papadaki A,Coelewij L,Adriani M,Nytrova P,Kubala Havrdova E,Fogdell-Hahn A,Farrell R,Dönnes P,Pineda-Torra I,Jury EC

56-0048-82 was used in Flow cytometry/Cell sorting to prove if serum lipids could contribute to ADA development by altering immune-cell lipid rafts.

Wed Apr 14 00:00:00 EDT 2021

Expression of CD20 after viral reactivation renders HIV-reservoir cells susceptible to Rituximab.

Nature communications

Serra-Peinado C,Grau-Expósito J,Luque-Ballesteros L,Astorga-Gamaza A,Navarro J,Gallego-Rodriguez J,Martin M,Curran A,Burgos J,Ribera E,Raventós B,Willekens R,Torrella A,Planas B,Badía R,Garcia F,Castellví J,Genescà M,Falcó V,Buzon MJ

Published figure using CD4 monoclonal antibody (Product # 56-0048-82) in Flow Cytometry

Fri Aug 16 00:00:00 EDT 2019

Fc Effector Function Contributes to the Activity of Human Anti-CTLA-4 Antibodies.

Cancer cell

Arce Vargas F,Furness AJS,Litchfield K,Joshi K,Rosenthal R,Ghorani E,Solomon I,Lesko MH,Ruef N,Roddie C,Henry JY,Spain L,Ben Aissa A,Georgiou A,Wong YNS,Smith M,Strauss D,Hayes A,Nicol D,O'Brien T,Mårtensson L,Ljungars A,Teige I,Frendéus B,Pule M,Marafioti T,Gore M,Larkin J,Turajlic S,Swanton C,Peggs KS,Quezada SA

56-0048 was used in Flow cytometry/Cell sorting to examine the importance of Fc-gamma receptor binding in contributing to the activity of anti-CTLA4 antibodies.

Mon Apr 09 00:00:00 EDT 2018

Accumulation of T-helper 22 cells, interleukin-22 and myeloid-derived suppressor cells promotes gastric cancer progression in elderly patients.

Oncology letters

Chen X,Wang Y,Wang J,Wen J,Jia X,Wang X,Zhang H

Published figure using CD4 monoclonal antibody (Product # 56-0048-82) in Flow Cytometry

Sun Jul 01 00:00:00 EDT 2018

IL-6 receptor blockade corrects defects of XIAP-deficient regulatory T cells.

Nature communications

Hsieh WC,Hsu TS,Chang YJ,Lai MZ

Published figure using CD4 monoclonal antibody (Product # 56-0048-82) in Flow Cytometry

Wed Jan 31 00:00:00 EST 2018

Complement receptor CD46 co-stimulates optimal human CD8⁺ T cell effector function via fatty acid metabolism.

Nature communications

Arbore G,West EE,Rahman J,Le Friec G,Niyonzima N,Pirooznia M,Tunc I,Pavlidis P,Powell N,Li Y,Liu P,Servais A,Couzi L,Fremeaux-Bacchi V,Placais L,Ferraro A,Walsh PR,Kavanagh D,Afzali B,Lavender P,Lachmann HJ,Kemper C

Published figure using CD4 monoclonal antibody (Product # 56-0048-82) in Flow Cytometry

Wed Oct 10 00:00:00 EDT 2018

The chimeric TAC receptor co-opts the T cell receptor yielding robust anti-tumor activity without toxicity.

Nature communications

Helsen CW,Hammill JA,Lau VWC,Mwawasi KA,Afsahi A,Bezverbnaya K,Newhook L,Hayes DL,Aarts C,Bojovic B,Denisova GF,Kwiecien JM,Brain I,Derocher H,Milne K,Nelson BH,Bramson JL

56-0048 was used in Flow cytometry/Cell sorting to show that T cell antigen coupler-engineered T cells may have a superior therapeutic index relative to chimeric antigen receptor-T cells.

Fri Aug 03 00:00:00 EDT 2018

The influence of chemotherapy on adenosine-producing B cells in patients with head and neck squamous cell carcinoma.

Oncotarget

Ziebart A,Huber U,Jeske S,Laban S,Doescher J,Hoffmann TK,Brunner C,Jackson EK,Schuler PJ

56-0048 was used in Flow cytometry/Cell sorting to study the influence of chemotherapy on the function of regulatory B cells.

Fri Jan 19 00:00:00 EST 2018

In Utero Transfer of Adeno-Associated Viral Vectors Produces Long-Term Factor IX Levels in a Cynomolgus Macaque Model.

Molecular therapy : the journal of the American Society of Gene Therapy

Mattar CNZ,Gil-Farina I,Rosales C,Johana N,Tan YYW,McIntosh J,Kaeppel C,Waddington SN,Biswas A,Choolani M,Schmidt M,Nathwani AC,Chan JKY

56-0048 was used in Flow cytometry/Cell sorting to report long-term transgene expression over more than 6 years without toxicity following a single intrauterine gene transfer at 0.9G using recombinant adeno-associated vector-human factor IX in the non-human primate model previously described.

Wed Aug 02 00:00:00 EDT 2017

Ex vivo culture of human atherosclerotic plaques: A model to study immune cells in atherogenesis.

Atherosclerosis

Lebedeva A,Vorobyeva D,Vagida M,Ivanova O,Felker E,Fitzgerald W,Danilova N,Gontarenko V,Shpektor A,Vasilieva E,Margolis L

Published figure using CD4 monoclonal antibody (Product # 56-0048-82) in Flow Cytometry

Fri Dec 01 00:00:00 EST 2017

Hormonal vitamin D up-regulates tissue-specific PD-L1 and PD-L2 surface glycoprotein expression in humans but not mice.

The Journal of biological chemistry

Dimitrov V,Bouttier M,Boukhaled G,Salehi-Tabar R,Avramescu RG,Memari B,Hasaj B,Lukacs GL,Krawczyk CM,White JH

Published figure using CD4 monoclonal antibody (Product # 56-0048-82) in Flow Cytometry

Fri Dec 15 00:00:00 EST 2017

Single-cell profiling reveals GPCR heterogeneity and functional patterning during neuroinflammation.

JCI insight

Tischner D,Grimm M,Kaur H,Staudenraus D,Carvalho J,Looso M,Günther S,Wanke F,Moos S,Siller N,Breuer J,Schwab N,Zipp F,Waisman A,Kurschus FC,Offermanns S,Wettschureck N

Published figure using CD4 monoclonal antibody (Product # 56-0048-82) in Flow Cytometry

Thu Aug 03 00:00:00 EDT 2017

Targeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis.

Stem cell reports

Li Y,Brauer PM,Singh J,Xhiku S,Yoganathan K,Zúñiga-Pflücker JC,Anderson MK

56-0048 was used in Flow cytometry/Cell sorting to identify HEB as a critical regulator of human mesodermal and hematopoietic specification.

Tue Sep 12 00:00:00 EDT 2017

PD-L1 expression and prognostic impact in glioblastoma.

Neuro-oncology

Nduom EK,Wei J,Yaghi NK,Huang N,Kong LY,Gabrusiewicz K,Ling X,Zhou S,Ivan C,Chen JQ,Burks JK,Fuller GN,Calin GA,Conrad CA,Creasy C,Ritthipichai K,Radvanyi L,Heimberger AB

Published figure using CD4 monoclonal antibody (Product # 56-0048-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

Tax and Semaphorin 4D Released from Lymphocytes Infected with Human Lymphotropic Virus Type 1 and Their Effect on Neurite Growth.

AIDS research and human retroviruses

Quintremil S,Alberti C,Rivera M,Medina F,Puente J,Cartier L,Ramírez E,Tanaka Y,Valenzuela MA

Published figure using CD4 monoclonal antibody (Product # 56-0048-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2016

Tumor-derived exosomes regulate expression of immune function-related genes in human T cell subsets.

Scientific reports

Muller L,Mitsuhashi M,Simms P,Gooding WE,Whiteside TL

Published figure using CD4 monoclonal antibody (Product # 56-0048-82) in Flow Cytometry

Thu Feb 04 00:00:00 EST 2016

Long-Term Stable Mixed Chimerism after Hematopoietic Stem Cell Transplantation in Patients with Non-Malignant Disease, Shall We Be Tolerant?

PloS one

Stikvoort A,Sundin M,Uzunel M,Gertow J,Sundberg B,Schaffer M,Mattsson J,Uhlin M

56-0048 was used in Flow cytometry/Cell sorting to study how the populations of NKT-cells differs following hematopoietic stem cell transplantation according to whether a patient retains any of their own immune cells or not.

Mon Jul 17 00:00:00 EDT 2017

The HIV-1 gp120 CD4-bound conformation is preferentially targeted by antibody-dependent cellular cytotoxicity-mediating antibodies in sera from HIV-1-infected individuals.

Journal of virology

Veillette M,Coutu M,Richard J,Batraville LA,Dagher O,Bernard N,Tremblay C,Kaufmann DE,Roger M,Finzi A

Published figure using CD4 monoclonal antibody (Product # 56-0048-82) in Flow Cytometry

Thu Jan 01 00:00:00 EST 2015

Impaired survival of regulatory T cells in pulmonary sarcoidosis.

Respiratory research

Broos CE,van Nimwegen M,Kleinjan A,ten Berge B,Muskens F,in 't Veen JC,Annema JT,Lambrecht BN,Hoogsteden HC,Hendriks RW,Kool M,van den Blink B

56-0048 was used in Flow cytometry/Cell sorting to investigate the role of impaired Treg function in sarcoidosis.

Wed Sep 16 00:00:00 EDT 2015

Aging and cytomegalovirus infection differentially and jointly affect distinct circulating T cell subsets in humans.

Journal of immunology (Baltimore, Md. : 1950)

Wertheimer AM,Bennett MS,Park B,Uhrlaub JL,Martinez C,Pulko V,Currier NL,Nikolich-Žugich D,Kaye J,Nikolich-Žugich J

56-0048 was used in Flow cytometry/Cell sorting to demonstrate that ageing and cytomegalovirus infection exert both distinct and joint influence upon blood T cell homeostasis in humans.

Sat Mar 01 00:00:00 EST 2014

An increased abundance of tumor-infiltrating regulatory T cells is correlated with the progression and prognosis of pancreatic ductal adenocarcinoma.

PloS one

Tang Y,Xu X,Guo S,Zhang C,Tang Y,Tian Y,Ni B,Lu B,Wang H

Published figure using CD4 monoclonal antibody (Product # 56-0048-82) in Immunohistochemistry

Tue Jan 06 00:00:00 EST 2015

Comparative immune phenotypic analysis of cutaneous Squamous Cell Carcinoma and Intraepidermal Carcinoma in immune-competent individuals: proportional representation of CD8+ T-cells but not FoxP3+ Regulatory T-cells is associated with disease stage.

PloS one

Freeman A,Bridge JA,Maruthayanar P,Overgaard NH,Jung JW,Simpson F,Prow TW,Soyer HP,Frazer IH,Freeman M,Wells JW

56-0048 was used in Flow cytometry/Cell sorting to define whether Intraepidermal Carcinoma and Squamous Cell Carcinoma have distinct immune profiles.

Mon Feb 01 00:00:00 EST 2016

Human neonatal naive CD4+ T cells have enhanced activation-dependent signaling regulated by the microRNA miR-181a.

Journal of immunology (Baltimore, Md. : 1950)

Palin AC,Ramachandran V,Acharya S,Lewis DB

56-0048 was used in Flow cytometry/Cell sorting to investigate the developmental differences in immune function between human neonates and adults, showing that neonatal naive CD4+ T cells have enhanced activation-dependent signalling.

Fri Mar 15 00:00:00 EDT 2013

Capsid-specific T-cell responses to natural infections with adeno-associated viruses in humans differ from those of nonhuman primates.

Molecular therapy : the journal of the American Society of Gene Therapy

Li H,Lasaro MO,Jia B,Lin SW,Haut LH,High KA,Ertl HC

56-0048 was used in Flow cytometry/Cell sorting to investigate whether rejection of AAV-transduced hepatocytes is caused by AAV capsid-specific CD8(+) T cells, showing that responses to adeno-associated viruses differ between humans and nonhuman primates.

Tue Nov 01 00:00:00 EDT 2011

CD4-CCR5 interaction in intracellular compartments contributes to receptor expression at the cell surface.

Blood

Achour L,Scott MG,Shirvani H,Thuret A,Bismuth G,Labbé-Jullié C,Marullo S

Published figure using CD4 monoclonal antibody (Product # 56-0048-82) in Flow Cytometry

Thu Feb 26 00:00:00 EST 2009

CD4-CCR5 interaction in intracellular compartments contributes to receptor expression at the cell surface.

Blood

Achour L,Scott MG,Shirvani H,Thuret A,Bismuth G,Labbé-Jullié C,Marullo S

Published figure using CD4 monoclonal antibody (Product # 56-0048-82) in Flow Cytometry

Thu Feb 26 00:00:00 EST 2009

CD4-CCR5 interaction in intracellular compartments contributes to receptor expression at the cell surface.

Blood

Achour L,Scott MG,Shirvani H,Thuret A,Bismuth G,Labbé-Jullié C,Marullo S

Published figure using CD4 monoclonal antibody (Product # 56-0048-82) in Flow Cytometry

Thu Feb 26 00:00:00 EST 2009

Bioinformatics

Protein Aliases: CD4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; cd4a; fCD4; Leu-3; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

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Gene Aliases: CD4; CD4mut

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UniProt ID: (Human) P01730

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Entrez Gene ID: (Human) 920

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Function(s)

virus receptor activity glycoprotein binding receptor activity transmembrane signaling receptor activity extracellular matrix structural constituent protein binding zinc ion binding coreceptor activity immunoglobulin binding enzyme binding protein kinase binding MHC class II protein binding protein homodimerization activity protein tyrosine kinase binding

Process(es)

cytokine production adaptive immune response induction by virus of host cell-cell fusion immune response signal transduction cell surface receptor signaling pathway enzyme linked receptor protein signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of calcium ion transport into cytosol fusion of virus membrane with host plasma membrane T cell differentiation entry into host cell T cell costimulation response to estradiol maintenance of protein location in cell response to vitamin D helper T cell enhancement of adaptive immune response positive regulation of T cell proliferation T cell selection positive regulation of interleukin-2 biosynthetic process positive regulation of protein kinase activity regulation of defense response to virus by virus positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-negative bacterium positive regulation of calcium-mediated signaling T cell receptor signaling pathway regulation of T cell activation

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CD4 Monoclonal Antibody (OKT4 (OKT-4)), Alexa Fluor™ 700, eBioscience™

CD4 Antibody (56-0048-82) in Flow

Product Details

56-0048-82

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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