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Nucleic Acid Gel Electrophoresis and Blotting |
Nucleic acid electrophoresis uses a gel matrix to separate DNA and RNA fragments based on size and molecular weight. In blotting, DNA and RNA fragments, separated by electrophoresis, are transferred to a membrane matrix. Probes are then used to detect the presence of target sequences on the membrane matrix. Specific DNA and RNA sequences in a sample are detected by Southern and Northern blots, respectively. Nucleic acid electrophoresis and blotting are chief pillars supporting molecular biology research. Lay a solid foundation for your research with Thermo Fisher Scientific’s suite of products that aid nucleic acid electrophoresis and blotting.
DNA
DNA electrophoresis
Isolate, quantify, and purify DNA fragments with highly acclaimed Invitrogen products for DNA electrophoresis including devices, gels, stains, ladders, markers, buffers, and reagents.
Southern blotting
Detect the identity, size, and abundance of target DNA sequences with superior performing Invitrogen products including membranes, probes, and buffers.
Thermo Scientific DNA electrophoresis
Conduct routine DNA electrophoresis with reliable Thermo Scientific products including GeneRuler ladders, TopVision agarose, loading dyes, and buffers.
RNA
RNA electrophoresis
Isolate, quantify, and purify RNA fragments with highly acclaimed Invitrogen products for denaturing and non-denaturing RNA electrophoresis.
Northern blotting
Immobilize and detect the identity, size, and abundance of target RNA sequences with highly acclaimed Invitrogen products for northern blotting.
Thermo Scientific RNA electrophoresis
Reliable products for routine RNA electrophoresis including RiboRuler RNA ladders, TopVision agarose, loading dyes, and buffers.
Nucleic acid electrophoresis systems
Thermo Fisher Scientific offers traditional and precast gel nucleic acid electrophoresis systems. The precast E-Gel systems come with a gel running platform integrated with an imaging device. These systems are suitable for both standard and high-throughput electrophoresis. The traditional Owl systems are used for casting and running micro, mini, and wide gels.
E-Gel Precast Agarose Gel Electrophoresis System
The Invitrogen E-Gel precast agarose gel electrophoresis systems are bufferless agarose gel electrophoresis systems with ready-to-use precast agarose gel cassettes with in-gel stain. The E-Gel precast agarose electrophoresis system simplifies the electrophoresis workflow, and it is ideal for analyzing PCR products, restriction digests, and plasmid preparations.
Owl Electrophoresis Systems
Products for conventional horizontal (agarose) or vertical (polyacrylamide) gel electrophoresis including tanks, chambers, casters, plates, spacers, combs, power supplies, and other accessories.
Resources
Nucleic acid electrophoresis products
Explore Thermo Fisher Scientific’s suite of products for all your electrophoresis needs.
Nucleic acid electrophoresis education
Learn about gel electrophoresis basics, workflow, considerations, applications, and troubleshooting for separation and analysis of nucleic acids.
DNA and RNA protocols
Find easy-to-follow protocols for everyday experiments, including agarose protocols and mRNA protocols.
Support
Nucleic acid purification and analysis support center
Find tips, troubleshooting help, and resources for your nucleic acid purification and analysis applications.
Contact us
For additional questions, reach out to our Technical Application Scientists.
For Research Use Only. Not for use in diagnostic procedures.
Stylesheet for Classic Wide Template adjustments
For Research Use Only. Not for use in diagnostic procedures.