Viral Nucleic Acid Isolation Kits

Silica-containing spin columns for viral RNA/DNA isolation are based on the well-known silica-binding properties of DNA and RNA. The spin column format makes washing the silica easy and efficient.

Our viral spin-column products including PureLink Viral RNA/DNA Mini Kit and GeneJET Viral DNA/RNA Purification Kit are designed for fast and easy isolation of viral RNA or DNA from cell-free samples such as serum, plasma, cerebrospinal fluid, and cell culture supernatant.

• Performs both viral RNA extraction and viral DNA extraction
• Yields highly concentrated viral nucleic acids for greater sensitivity in detection
• Purified nucleic acid is ready for real-time PCR and endpoint analysis
   

Magnetic bead-based viral nucleic acid extraction kits utilize small paramagnetic beads that strongly yet reversibly bind to DNA/RNA. Benefits of bead-based MagMAX Viral and Pathogen Nucleic Acid Isolation Kits include:

• Magnetic beads bind RNA more efficiently than glass fiber filters, resulting in higher and more consistent RNA yields.
• Magnetic beads can be efficiently transferred and washed by pipette, making the process easily automatable.
• Magnetic beads are more flexible with regard to sample type, i.e., whole blood and cells
• Viral nucleic acid can be eluted into a small volume of highly purified material suitable for NGS
   

All of our viral spin-column kits including PureLink Viral RNA/DNA Mini Kit, Thermo Scientific GeneJET Viral DNA/RNA Purification Kit and PureLink Pro 96 Viral RNA/DNA Kit with 96-well plate are designed for fast and easy isolation of viral RNA or DNA from cell-free samples such as serum, plasma, cerebrospinal fluid, and cell culture supernatant.

• Complete solution for both viral RNA and DNA
• Yields highly concentrated viral nucleic acids for greater sensitivity in detection
• Ideal for real-time PCR and endpoint analysis

Some viral kits are experiencing long lead times but there is another option. See our automated isolation solution using MagMAX Viral/Pathogen Nucleic Acid Isolation kits.

Viral nucleic extraction kits from Thermo Fisher Scientific are optimized to provide maximum viral DNA/RNA yield, purity, and integrity from a broad range of sample types. Choose the kit that best suits your research needs:

Best for	Recovery of low abundance viral DNA/RNA	High quality DNA/RNA from liquid samples	Automated extraction of viral DNA/RNA from liquid samples	Automated extraction of DNA/RNA from range of biological sample types	DNA/RNA extraction from difficult to lyse samples
Product	PureLink Viral RNA/DNA Mini Kit	GeneJet Viral DNA/RNA Purification Kit	PureLink Pro 96 Viral RNA/DNA Purification Kit	MagMAX Prime Viral/Pathogen NA Isolation Kit	MagMAX Microbiome Ultra Nucleic Acid Isolation Kit
Cat. No.	12280050	K0821	12280096A	A58145	A42357
Starting volume	500 µL	200–400 µL	200 µL	200–400 µL	200–500 µL or 100–250 mg
Sample types	Cell-free plasma, serum, cerebrospinal fluid	Plasma, serum, nasal swab, buccal swab, saliva, urine	Cell-free plasma, serum, cerebrospinal fluid	Swabs in transport media (respiratory, genital and lesion swabs), saliva, stool, urine, plasma and serum	Stool, swabs, transport media, culture media, urine, wastewater, soil
Isolation technology & format	silica spin column	silica spin column	silica- membrane 96-well microplate	magnetic beads/ deep-well plate	magnetic beads/ multiwell plate
Protocol time	45 mins	30–60 mins	30 mins	30–60 mins (automated)	~60 mins
Sample elution volume	10–50 µL	50 µL	50–150 µL	60 µL	50–200 μL
Automation compatible	No	No	Yes	Yes	Yes
Number of preps	50	50	384	600	100
Downstream applications	(RT)-PCR, (RT)-qPCR, Northern Blotting	(RT)-PCR, (RT)-qPCR, Northern Blotting	(RT)-PCR, (RT)-qPCR, Northern Blotting	(RT)-PCR, (RT)-qPCR, NGS	(RT)-PCR, (RT)-qPCR, NGS

Need to isolate intact virus? Visit SARS-CoV-2 Virus Purification to learn how

For fast, easy, and effective high-throughput sample processing, automate your nucleic acid isolation workflow. KingFisher automated sample purification systems can be used to process all MagMAX kits, including MagMAX Viral/Pathogen Nucleic Acid Isolation Kits and MagMAX Prime Viral/Pathogen NA Isolation Kit.

Automated viral DNA extraction protocols for the silica plate-based PureLink Pro 96 Viral RNA/DNA Purification Kit use a vacuum manifold to achieve buffer flow/wash and are available for a variety of XYZ liquid handlers.

The RNA purification process presents unique challenges, one of which is ribonuclease contamination. Even a trace amount of RNase contamination can sabotage your experiment. Our RNAse-free Reagents and Supplies are designed to be used together to help avoid, detect, and inhibit RNases and nucleases in your lab.