Purifique o DNA do plasmídeo para todas as aplicações

Nossos kits de isolamento de plasmídeo Invitrogen oferecem opções para eliminar os desafios levantados pelo processamento de múltiplas amostras. Projetamos nossos produtos de extração de DNA plasmídico para isolar o DNA plasmídeo na pureza e escala necessárias. Escolha entre nossa ampla gama de tecnologias de purificação de plasmídeos de alto desempenho e custo-benefício projetadas para ajudá-lo a superar problemas comuns de preparação de plasmídeos, como baixa recuperação e presença de impurezas.

What is plasmid purification?

Plasmid purification, also known as plasmid isolation or plasmid DNA isolation, refers to the process of extracting and purifying plasmid DNA from bacterial cells, allowing scientists to obtain a highly concentrated and pure form of the plasmid for further analysis and experimentation. The key steps in plasmid purification typically include bacterial cell lysis, removal of cellular debris, binding of plasmid DNA, washing, elution of plasmid DNA, and optional additional steps depending on the downstream application.

Qual kit de isolamento e purificação de DNA de plasmídeo é adequado para você?

 Rápido isolamento da qualidade da biologia molecularPadrão ouro para ultrapura,
alta qualidade
Pureza aprimorada para aplicativos sensíveis
Grade de PurificaçãoMolecularTransfecçãoTransfecção Avançada
EndotoxinaPadrão (>10 EU/µg)Baixa endotoxina (0.1–1 EU/µg)Endotoxin-free
(< 0.1 EU/µg)
Kit de purificação de DNA plasmídeoGeneJETPureLink HiPurePureLink FastPureLinkExpi
Endotoxin-Free
Total protocol time15–60 min30–120 min30 min90–120 min
Tamanho da prepaçãoMini–MaxiMini–GigaMidi, MaxiMaxi, Mega, Giga
Rendimento até20 µg–1 mg20 µg–15 mg0.4 mg (midi prep) or 1.5 mg (maxi prep)1.5–15 mg
TecnologiaSilica membraneAnion exchangeAdvanced silicaAnion exchange membrane
AplicaçõesNão Sensivel
  • PCR
  • Cloning (digestion, ligation)
  • Sequencing
Sensivel
  • Transfection of standard, robust cell lines
  • All molecular biology applications
  • Muito Sensivel
  • Transfecção primária e de células
  • Gene therapy and vaccine (in vivo) research
  • All standard transfection applications
  • All molecular biology applications
 Compre agoraCompre agoraCompre agoraCompre agora

Tamanhos de preparação de DNA de plasmídeo

 MiniprepMidiprepMaxiprepMegaprepGigaprep
Volume da cultura bacteriana durante a noite1–5 mL25–100 mL100–500 mL500 mL–2.5 L2.5–5 L
Rendimento aproximadoaté 40 µgaté 400 µgaté 1.5 mgaté 5 mgaté 15 mg


Purity grade and endotoxin level for plasmid DNA isolation

When it comes to plasmid isolation, scientists have a range of options depending on their specific needs. Molecular-grade plasmid isolation technologies offer speed and cost-effectiveness, making them excellent for robust applications like cloning, nucleic acid labeling, PCR, and sequencing, where high purity levels are not essential. On the other hand, transfection-grade plasmid purification technologies are designed for applications that require greater purity and higher yields, such as transfection of standard cell lines and in vitro transcription. Transfection-grade plasmid DNA can also be used for molecular-grade applications. For the most sensitive applications, such as transfection of sensitive cell lines or in vivo research, advanced transfection-grade plasmid isolation technology is recommended. This technology helps ensure the highest purity and can also be used for all molecular and transfection-grade applications.

Purity gradeMolecularTransfectionAdvanced transfection
Endotoxin level>10 EU/µg0.1–1 EU/µg<0.1 EU/µg

A purificação robusta do DNA do plasmídeo, na quantidade e pureza necessárias para a aplicação a jusante de interesse, é a etapa principal do fluxo de trabalho da expressão de proteínas. Saiba mais sobre como você pode obter o plasmídeo de grau de transfecção e usá-lo com os sistemas de expressão transitória em mamíferos Gibco ExpiCHO e Expi293.

Baixe o Guia de DNA Baixe notas de aplicação  Baixe nosso guia de seleção


Solutions for automated plasmid purification

Automated plasmid purification refers to the use of advanced robotics, liquid handling capabilities, and specialized magnetic-bead purification kits to help streamline the isolation of plasmid DNA from bacterial cultures. These solutions can handle multiple samples simultaneously, helping increase throughput and reducing manual processing time. They also help minimize human error to enable reproducibility by precisely controlling the purification steps and volumes of reagents used. Thermo Fisher offers automated plasmid purification solutions that employ innovative technologies that allow for high-throughput, centrifuge-free purification of transfection-grade plasmid DNA with minimal hands-on steps. This purified pDNA can then be used for various downstream applications, such as transfection into sensitive cell lines, preparation of short hairpin vectors, enzymatic modifications, molecular cloning, restriction digestion, sequencing, and in vitro transcription/translation.

 

Applied Biosystems MagMAX Pro HT NoSpin Miniprep Plasmid Purification Kit

Thermo Scientific KingFisher Plasmid Pro

image of magmax pro ht no spin kitimage of plasmid pro instrument
Prep sizes availableMiniMaxi
Purity grade/Endotoxin levelTransfection grade
(0.1–1 EU/µg)
Transfection grade
(0.1–1 EU/µg)


Kits for transfection-grade plasmid DNA purification

Transfection-grade plasmid isolation kits are specifically designed for scientists who need to extract plasmid DNA (pDNA) with minimal levels of endotoxins from bacterial cultures. During the plasmid purification process, large amounts of endotoxins are released from lysed bacteria, which can negatively impact cell growth, cell viability, transfection efficiency, protein expression, and even mimic sepsis. To address these concerns, our kits designed for low-endotoxin plasmid DNA isolation carefully remove these endotoxins, helping ensure the integrity of the isolated plasmid DNA. These kits offer a fast and easy method of plasmid purification, helping provide high purity and yield for sensitive downstream applications. Whether you are working with small or large prep sizes, our transfection-grade plasmid isolation kits are tailored to help meet your specific needs, enabling you achieve reliable and reproducible results. 

 Invitrogen PureLink Fast Low-Endotoxin Purification KitsInvitrogen PureLink HiPure Plasmid KitsPureLink HiPure Plasmid Filter Kits
 productproductPureLink HiPure Plasmid Filter Midiprep Kit
Prep sizes availableMidi, MaxiMini, Midi, Maxi, Mega, GigaMidiMaxi
Purity grade/Endotoxin levelTransfection grade
(0.1–1 EU/µg)
Transfection grade
(0.1–1 EU/µg)
Transfection grade
(0.1–1 EU/µg)


Kits for advanced transfection-grade plasmid DNA purification

For scientists who require an even higher level of endotoxin removal, we offer advanced transfection-grade plasmid isolation kits. These kits go beyond the standard transfection-grade kits and provide an enhanced level of endotoxin removal, helping ensure the utmost purity of the isolated plasmid DNA. With our advanced transfection-grade plasmid isolation kits, you can confidently perform downstream applications that demand the highest quality pDNA. These kits are excellent for researchers working on critical experiments where even trace amounts of endotoxins can have a significant impact on their results. Using our advanced-transfection grade plasmid isolation kits can help eliminate the risks associated with endotoxins and obtain pure plasmid DNA for your scientific endeavors.

 Thermo Scientific GeneJET Endo-Free Plasmid Maxiprep KitInvitrogen PureLink Expi Endotoxin-Free Plasmid Purification Kits
 productproduct
Prep sizes availableMaxiMaxi, Mega, Giga
Purity grade/Endotoxin levelAdvanced transfection grade
(<0.1 EU/µg)
Advanced transfection grade
(<0.1 EU/µg)


Kits for molecular-grade plasmid purification

For fast, easy, and reliable plasmid purification, our Invitrogen PureLink and GeneJET molecular-grade plasmid DNA (pDNA) isolation kits produce pure, ready-to-use plasmids that are compatible with a range of standard molecular biology applications. Molecular-grade kits are suitable for transformation of bacterial and yeast cells; however, they may not be well suited for transfection of sensitive mammalian cells due to the presence of endotoxins. For transfection applications, please see the transfection-grade plasmid purification kits above.

 Invitrogen PureLink Pro Quick 96 Plasmid Purification KitInvitrogen PureLink Quick Plasmid Miniprep KitThermo Scientific GeneJET Plasmid Kits
 productproductproduct
Prep size availableSmall-scale 96-well plate formatMiniMini, Midi, Maxi
Purity grade/Endotoxin levelMolecular grade
(>10 EU/µg)
Molecular grade
(>10 EU/µg)
Molecular grade
(>10 EU/µg)


Additional buffers for plasmid isolation


Plasmid purification—How-to videos

Purificar o DNA do plasmídeo com PureLink e GeneJet

Como purificar o DNA plasmídico livre de endotoxina

Assista a este vídeo para aprender como purificar o DNA do plasmídeo usando o PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit. Este vídeo mostra como isolar e purificar o DNA plasmídico de alta qualidade e livre de endotoxina para aplicações sensíveis como a transfecção primária de células.

Como purificar DNA de plasmídeo de grau molecular

Este vídeo mostra como purificar o DNA plasmídico de grau molecular em 15 minutos usando nosso GeneJET Plasmid Miniprep Kit. O DNA plasmídico de grau molecular é adequado para clonagem, marcação de ácidos nucleicos, PCR, sequenciamento de DNA e transformação.

Como purificar o DNA do plasmídeo usando nosso kit megaprep

Isolar o DNA do plasmídeo de grau de transfecção em menos de 90 minutos com o InvitrogenPureLink HiPure Expi Plasmid Megaprep Kit. Essa inovadora resina proprietária de troca aniônica nesses kits combina grande capacidade com taxa de fluxo rápida, alta resolução e eficiência.

Como purificar DNA de plasmídeo de grau de transfecção

Aprenda a purificar o DNA do plasmídeo de grau de transfecção com o InvitrogenPureLink HiPure Plasmid Filter Maxiprep Kit. O DNA do plasmídeo de grau de transfecção é adequado para todas as aplicações de grau molecular, bem como transcrição e transfecção in vitro.

Resources
  • Protocol Videos—Videos to help you isolate nucleic acids using a variety of techniques.
  • DNA & RNA Selection Guide—Find the right purification products.
  • Videos—View our library of DNA & RNA purification and analysis videos.
  • Application Notes—Explore our application notes from scientists sharing data for isolation products
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For Research Use Only. Not for use in diagnostic procedures.

For Research Use Only. Not for use in diagnostic procedures.