Antibody Protocol Collection

In this collection, protocols for each antibody application are provided along with links to antibodies, reagents, and resources. Learn how to perform each application so quality, consistent results are attained every time.
 

Western Blot Protocols

Western blot (WB) is a technique that separates a mixture of proteins via size exclusion electrophoresis. The target protein is identified using an antibody specific for the target and is visualized as a band via chemiluminescent or fluorescent detection.

Shop WB antibodies  |  Shop WB reagents  |  Blot Builder  |  WB troubleshooting
Western blot antibody dilution calculator

Flow Cytometry Protocols

Flow cytometry identifies cellular characteristics and expression of proteins in a heterogeneous cell population by flowing one cell at a time past a laser beam.

Shop Flow antibodies  |  Shop Flow reagents  |  Panel Builder  |  SpectraViewer

Immunohistochemistry Protocols

Immunohistochemistry (IHC) identifies structures in whole tissue samples. A mixture of antibodies and stains are used to microscopically visualize the cells.

Shop IHC antibodies  |  IHC troubleshooting
 Download IHC guide

Immunocytochemistry/Immunofluorescence Protocols

Immunocytochemistry/Immunofluorescence (ICC/IF) identifies cells from samples that are no longer in their natural state. Antibodies are used to microscopically visualize cellular structures.

Shop ICC/IF antibodies  |  ICC/IF troubleshooting  |  SpectraViewer

ELISA Protocols

Enzyme-linked immunosorbent assay (ELISA) identifies protein concentrations using antibodies and a plate with multiple sample wells. Visualization is performed using colorimetric or fluorescent enzymatic substrates and is represented graphically.

ELISA Overview  |  Shop ELISA antibodies  |  Shop ELISA kits  |  Shop ELISA reagents
Download Biomarker guide

Chromatin Immunoprecipitation Protocols

Chromatin immunoprecipitation (ChIP) analyzes protein-DNA interactions. The target interactions are identified using antibodies and the DNA is amplified for graphical visualization.

Shop ChIP antibodies
 Download ChIP guide  |   Protein preparation handbook

Immunoprecipitation Protocols

Immunoprecipitation (IP) precipitates a target protein from a mixture of proteins using an antibody. Immunoprecipitation can be used in conjunction with other assays or independently.

Shop IP antibodies  |  Shop IP beads, resins, kits  |  IP troubleshooting
 Protein preparation handbook