Biotin labeled rabbit IgG cross-adsorbed secondary antibody in immunofluorescence

Biotin-labeled secondary antibodies are commonly used in various applications such as western blotting, ELISA, immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), and flow cytometry. Secondary antibodies labeled with multiple biotin molecules, when combined with a streptavidin- or avidin-based conjugates, allow for signal amplification of lowly expressed proteins. Search below our wide-ranging catalog of biotinylated secondary antibodies that can be used with fluorescent or enzymatic streptavidin- or avidin-based techniques.

Search all Biotin-Labeled Secondary Antibodies

Biotinylated secondary antibodies by application

Navigate the tables below to see our wide-ranging offerings for biotinylated secondary antibodies by application.

Need a fluorescent substrate for immunofluorescence, immunohistochemistry, or in situ hybridization applications? Check out our Tyramide SuperBoost Kits to amplify your signal!
 

Table 1. Biotinylated secondary antibodies for western blotting

Target speciesHost
GoatRabbitMouseDonkeyChickenBovineHorseRatSheep
Anti-MouseBiotin-labeled goat anti-mouseBiotin-labeled rabbit anti-mouseBiotin-labeled donkey anti-mouseBiotin-labeled chicken anti-mouseBiotin-labeled bovine anti-mouseBiotin-labeled horse anti-mouseBiotin-labeled rat anti-mouse
Anti-HumanBiotin-labeled goat anti-humanBiotin-labeled rabbit anti-humanBiotin-labeled mouse anti-humanBiotin-labeled chicken anti-humanBiotin-labeled bovine anti-human
Anti-RabbitBiotin-labeled goat anti-rabbitBiotin-labeled mouse anti-rabbitBiotin-labeled donkey anti-rabbitBiotin-labeled chicken anti-rabbit
Anti-RatBiotin-labeled goat anti-ratBiotin-labeled rabbit anti-ratBiotin-labeled mouse anti-ratBiotin-labeled donkey anti-ratBiotin-labeled chicken anti-ratBiotin-labeled sheep anti-rat
Anti-GoatBiotin-labeled rabbit anti-goatBiotin-labeled mouse anti-goatBiotin-labeled donkey anti-goat
Anti-BovineBiotin-labeled rabbit anti-bovine
Anti-ChickenBiotin-labeled goat anti-chickenBiotin-labeled rabbit anti-chickenBiotin-labeled donkey anti-chickenBiotin-labeled bovine anti-chicken
Anti-HamsterBiotin-labeled goat anti-hamster
Anti-Guinea PigBiotin-labeled rabbit anti-guinea pig
Anti-SheepBiotin-labeled rabbit anti-sheep
Anti-DonkeyBiotin-labeled goat anti-donkeyBiotin-labeled rabbit anti-donkey
Anti-Horse

Biotin-labeled goat anti-horse

Table 2. Biotinylated secondary antibodies for ELISA applications

Target speciesHost
GoatRabbitMouseDonkeyChickenRatBovineSheep
Anti-MouseBiotin-labeled goat anti-mouseBiotin-labeled rabbit anti-mouseBiotin-labeled donkey anti-mouseBiotin-labeled chicken anti-mouseBiotin-labeled rat anti-mouseBiotin-labeled bovine anti-mouse
Anti-HumanBiotin-labeled goat anti-humanBiotin-labeled rabbit anti-humanBiotin-labeled mouse anti-humanBiotin-labeled chicken anti-humanBiotin-labeled bovine anti-human
Anti-RabbitBiotin-labeled goat anti-rabbitBiotin-labeled mouse anti-rabbitBiotin-labeled donkey anti-rabbitBiotin-labeled chicken anti-rabbit
Anti-RatBiotin-labeled goat anti-ratBiotin-labeled rabbit anti-ratBiotin-labeled mouse anti-ratBiotin-labeled donkey anti-ratBiotin-labeled chicken anti-ratBiotin-labeled sheep anti-rat
Anti-GoatBiotin-labeled rabbit anti-goatBiotin-labeled mouse anti-goatBiotin-labeled donkey anti-goatBiotin-labeled chicken anti-goat
Anti-BovineBiotin-labeled rabbit anti-bovineBiotin-labeled chicken anti-bovine
Anti-ChickenBiotin-labeled goat anti-chickenBiotin-labeled rabbit anti-chickenBiotin-labeled donkey anti-chickenBiotin-labeled bovine anti-chicken
Anti-HamsterBiotin-labeled goat anti-hamsterBiotin-labeled rabbit anti-hamster
Anti-Guinea PigBiotin-labeled goat anti-guinea pigBiotin-labeled rabbit anti-guinea pig
Anti-SheepBiotin-labeled donkey anti-sheep
Anti-DogBiotin-labeled goat anti-dog
Anti-CamelidBiotin-labeled goat anti-llamaBiotin-labeled rabbit anti-llama
Anti-DonkeyBiotin-labeled goat anti-donkeyBiotin-labeled rabbit anti-donkey
Anti-Horse

Biotin-labeled goat anti-horse

Anti-Non-human primateBiotin-labeled mouse anti-non-human primate

Table 5. Biotinylated secondary antibodies for immunofluorescence applications

Target speciesHost
GoatRabbitMouseDonkeyChickenRatHorseSheep
Anti-MouseBiotin-labeled goat anti-mouseBiotin-labeled rabbit anti-mouseBiotin-labeled donkey anti-mouseBiotin-labeled chicken anti-mouseBiotin-labeled rat anti-mouseBiotin-labeled horse anti-mouse
Anti-HumanBiotin-labeled goat anti-humanBiotin-labeled rabbit anti-humanBiotin-labeled mouse anti-humanBiotin-labeled sheep anti-human
Anti-RabbitBiotin-labeled goat anti-rabbitBiotin-labeled mouse anti-rabbitBiotin-labeled donkey anti-rabbitBiotin-labeled chicken anti-rabbit
Anti-RatBiotin-labeled goat anti-ratBiotin-labeled rabbit anti-ratBiotin-labeled mouse anti-ratBiotin-labeled donkey anti-ratBiotin-labeled chicken anti-rat
Anti-GoatBiotin-labeled rabbit anti-goatBiotin-labeled mouse anti-goatBiotin-labeled donkey anti-goatBiotin-labeled chicken anti-goat
Anti-BovineBiotin-labeled rabbit anti-bovine
Anti-ChickenBiotin-labeled goat anti-chickenBiotin-labeled rabbit anti-chicken
Anti-HamsterBiotin-labeled goat anti-hamster

Biotin-labeled rabbit anti-hamster

Anti-Guinea PigBiotin-labeled goat anti-guinea pig
Anti-SheepBiotin-labeled rabbit anti-sheepBiotin-labeled donkey anti-sheep
Anti-DogBiotin-labeled goat anti-dog
Anti-CamelidBiotin-labeled goat anti-llamaBiotin-labeled rabbit anti-llama
Anti-HorseBiotin-labeled goat anti-horse

Table 1. Biotinylated secondary antibodies for western blotting

Target speciesHost
GoatRabbitMouseDonkeyChickenBovineHorseRatSheep
Anti-MouseBiotin-labeled goat anti-mouseBiotin-labeled rabbit anti-mouseBiotin-labeled donkey anti-mouseBiotin-labeled chicken anti-mouseBiotin-labeled bovine anti-mouseBiotin-labeled horse anti-mouseBiotin-labeled rat anti-mouse
Anti-HumanBiotin-labeled goat anti-humanBiotin-labeled rabbit anti-humanBiotin-labeled mouse anti-humanBiotin-labeled chicken anti-humanBiotin-labeled bovine anti-human
Anti-RabbitBiotin-labeled goat anti-rabbitBiotin-labeled mouse anti-rabbitBiotin-labeled donkey anti-rabbitBiotin-labeled chicken anti-rabbit
Anti-RatBiotin-labeled goat anti-ratBiotin-labeled rabbit anti-ratBiotin-labeled mouse anti-ratBiotin-labeled donkey anti-ratBiotin-labeled chicken anti-ratBiotin-labeled sheep anti-rat
Anti-GoatBiotin-labeled rabbit anti-goatBiotin-labeled mouse anti-goatBiotin-labeled donkey anti-goat
Anti-BovineBiotin-labeled rabbit anti-bovine
Anti-ChickenBiotin-labeled goat anti-chickenBiotin-labeled rabbit anti-chickenBiotin-labeled donkey anti-chickenBiotin-labeled bovine anti-chicken
Anti-HamsterBiotin-labeled goat anti-hamster
Anti-Guinea PigBiotin-labeled rabbit anti-guinea pig
Anti-SheepBiotin-labeled rabbit anti-sheep
Anti-DonkeyBiotin-labeled goat anti-donkeyBiotin-labeled rabbit anti-donkey
Anti-Horse

Biotin-labeled goat anti-horse

Table 2. Biotinylated secondary antibodies for ELISA applications

Target speciesHost
GoatRabbitMouseDonkeyChickenRatBovineSheep
Anti-MouseBiotin-labeled goat anti-mouseBiotin-labeled rabbit anti-mouseBiotin-labeled donkey anti-mouseBiotin-labeled chicken anti-mouseBiotin-labeled rat anti-mouseBiotin-labeled bovine anti-mouse
Anti-HumanBiotin-labeled goat anti-humanBiotin-labeled rabbit anti-humanBiotin-labeled mouse anti-humanBiotin-labeled chicken anti-humanBiotin-labeled bovine anti-human
Anti-RabbitBiotin-labeled goat anti-rabbitBiotin-labeled mouse anti-rabbitBiotin-labeled donkey anti-rabbitBiotin-labeled chicken anti-rabbit
Anti-RatBiotin-labeled goat anti-ratBiotin-labeled rabbit anti-ratBiotin-labeled mouse anti-ratBiotin-labeled donkey anti-ratBiotin-labeled chicken anti-ratBiotin-labeled sheep anti-rat
Anti-GoatBiotin-labeled rabbit anti-goatBiotin-labeled mouse anti-goatBiotin-labeled donkey anti-goatBiotin-labeled chicken anti-goat
Anti-BovineBiotin-labeled rabbit anti-bovineBiotin-labeled chicken anti-bovine
Anti-ChickenBiotin-labeled goat anti-chickenBiotin-labeled rabbit anti-chickenBiotin-labeled donkey anti-chickenBiotin-labeled bovine anti-chicken
Anti-HamsterBiotin-labeled goat anti-hamsterBiotin-labeled rabbit anti-hamster
Anti-Guinea PigBiotin-labeled goat anti-guinea pigBiotin-labeled rabbit anti-guinea pig
Anti-SheepBiotin-labeled donkey anti-sheep
Anti-DogBiotin-labeled goat anti-dog
Anti-CamelidBiotin-labeled goat anti-llamaBiotin-labeled rabbit anti-llama
Anti-DonkeyBiotin-labeled goat anti-donkeyBiotin-labeled rabbit anti-donkey
Anti-Horse

Biotin-labeled goat anti-horse

Anti-Non-human primateBiotin-labeled mouse anti-non-human primate

Table 5. Biotinylated secondary antibodies for immunofluorescence applications

Target speciesHost
GoatRabbitMouseDonkeyChickenRatHorseSheep
Anti-MouseBiotin-labeled goat anti-mouseBiotin-labeled rabbit anti-mouseBiotin-labeled donkey anti-mouseBiotin-labeled chicken anti-mouseBiotin-labeled rat anti-mouseBiotin-labeled horse anti-mouse
Anti-HumanBiotin-labeled goat anti-humanBiotin-labeled rabbit anti-humanBiotin-labeled mouse anti-humanBiotin-labeled sheep anti-human
Anti-RabbitBiotin-labeled goat anti-rabbitBiotin-labeled mouse anti-rabbitBiotin-labeled donkey anti-rabbitBiotin-labeled chicken anti-rabbit
Anti-RatBiotin-labeled goat anti-ratBiotin-labeled rabbit anti-ratBiotin-labeled mouse anti-ratBiotin-labeled donkey anti-ratBiotin-labeled chicken anti-rat
Anti-GoatBiotin-labeled rabbit anti-goatBiotin-labeled mouse anti-goatBiotin-labeled donkey anti-goatBiotin-labeled chicken anti-goat
Anti-BovineBiotin-labeled rabbit anti-bovine
Anti-ChickenBiotin-labeled goat anti-chickenBiotin-labeled rabbit anti-chicken
Anti-HamsterBiotin-labeled goat anti-hamster

Biotin-labeled rabbit anti-hamster

Anti-Guinea PigBiotin-labeled goat anti-guinea pig
Anti-SheepBiotin-labeled rabbit anti-sheepBiotin-labeled donkey anti-sheep
Anti-DogBiotin-labeled goat anti-dog
Anti-CamelidBiotin-labeled goat anti-llamaBiotin-labeled rabbit anti-llama
Anti-HorseBiotin-labeled goat anti-horse


Biotinylated reagents for improved protein binding

Biotinylated reagents including biotin, biotin-X, and biotin-XX that are conjugated to secondary antibodies can be used to enhance subsequent binding to proteins such as streptavidin and avidin. Products that are denoted with an ‘X’ represent those that have a seven-atom aminohexanoyl spacer between biotin and the reactive carboxylic acid. This spacer helps to enhance binding to the biotin-binding pockets of avidin and streptavidin. DSB-X Biotin reagent also utilizes a seven-atom spacer to increase binding to streptavidin, avidin, or other biotin-binding proteins. However, DSB-X biotin conjugates can be reversibly bound to streptavidin or avidin, leaving the molecule of interest unchanged.


Tyramide SuperBoost technology for use with biotin labeled secondary antibodies

Tyramide SuperBoost Signal Amplification is a highly sensitive method for the detection of low-abundance targets in multiplexable fluorescent IHC/ICC/ISH experiments. SuperBoost kits combine the brightness of Alexa Fluor dyes with the enhancement of tyramide signal amplification to produce a superior signal. For use with biotin conjugated antibodies use one of the Alexa Fluor Tyramide SuperBoost kits with streptavidin in table X below.

Find all Alexa Fluor streptavidin and avidin conjugates for biotin binding

Amplify the signal of your biotin labeled antibody using the Biotin XX Tyramide SuperBoost Kit, Streptavidin (Cat. No. B40931). This specific kit contains biotin XX tyramide with HRP-conjugated streptavidin, which is used prior to detection and signal amplification with an Alexa Fluor-conjugated streptavidin molecule. The table below provides various Alexa Fluor-streptavidin conjugates for use with Biotin XX Tyramide SuperBoost Kit, Streptavidin.

Detection of ATP Synthase using Biotin XX Tyramide SuperBoost Kit, Streptavidin with Alexa Fluor 488 Streptavidin

Figure 1. ATP Synthase detection with Biotin XX Tyramide SuperBoost Kit. HeLa cells were fixed and permeabilized with Image-iT Fixation/Permeabilization Kit (Cat. No R37602). For ATP synthase detection, cells were labeled with anti-ATP Synthase primary antibody and a biotin-conjugated secondary antibody. Biotin signal was enhanced using Biotin XX Tyramide SuperBoost Kit – Streptavidin (Cat. No B40931) and detected with Alexa Fluor 488 Streptavidin (Cat. No. S11223). For best antifade protection, cover slip was mounted with ProLong Diamond Antifade Mountant (Cat. No. P36961).