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Biotin-Labeled Secondary Antibodies
Biotin-labeled secondary antibodies are commonly used in various applications such as western blotting, ELISA, immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), and flow cytometry. Secondary antibodies labeled with multiple biotin molecules, when combined with a streptavidin- or avidin-based conjugates, allow for signal amplification of lowly expressed proteins. Search below our wide-ranging catalog of biotinylated secondary antibodies that can be used with fluorescent or enzymatic streptavidin- or avidin-based techniques.
Biotinylated secondary antibodies by application
Navigate the tables below to see our wide-ranging offerings for biotinylated secondary antibodies by application.
Need a fluorescent substrate for immunofluorescence, immunohistochemistry, or in situ hybridization applications? Check out our Tyramide SuperBoost Kits to amplify your signal!
Table 1. Biotinylated secondary antibodies for western blotting
Table 2. Biotinylated secondary antibodies for ELISA applications
Table 3. Biotinylated secondary antibodies for IHC
Table 4. Biotinylated secondary antibodies for ICC
| Target species | Host | ||||||
|---|---|---|---|---|---|---|---|
| Goat | Rabbit | Mouse | Donkey | Rat | Bovine | Horse | |
| Anti-Mouse | Biotin labeled goat anti-mouse | Biotin labeled rabbit anti-mouse | — | — | Biotin labeled rat anti-mouse | — | Biotin labeled horse anti-mouse |
| Anti-Human | Biotin labeled goat anti-human | Biotin labeled rabbit anti-human | Biotin labeled mouse anti-human | — | — | Biotin labeled bovine anti-human | — |
| Anti-Rabbit | Biotin labeled goat anti-rabbit | — | Biotin labeled mouse anti-rabbit | Biotin labeled donkey anti-rabbit | — | — | — |
| Anti-Rat | Biotin labeled goat anti-rat | Biotin labeled rabbit anti-rat | Biotin labeled mouse anti-rat | — | — | — | — |
| Anti-Goat | — | Biotin labeled rabbit anti-goat | Biotin labeled mouse anti-goat | — | — | — | — |
| Anti-Chicken | — | Biotin labeled rabbit anti-chicken | — | — | — | Biotin labeled bovine anti-chicken | — |
| Anti-Hamster | Biotin labeled goat anti-hamster | — | — | — | — | — | — |
| Anti-Sheep | — | Biotin labeled rabbit anti-sheep | — | — | — | — | — |
| Anti-Horse | Biotin labeled goat anti-horse | — | — | — | — | — | — |
Table 5. Biotinylated secondary antibodies for immunofluorescence applications
Table 6. Biotinylated secondary antibodies for flow cytometry
Table 1. Biotinylated secondary antibodies for western blotting
Table 2. Biotinylated secondary antibodies for ELISA applications
Table 3. Biotinylated secondary antibodies for IHC
Table 4. Biotinylated secondary antibodies for ICC
| Target species | Host | ||||||
|---|---|---|---|---|---|---|---|
| Goat | Rabbit | Mouse | Donkey | Rat | Bovine | Horse | |
| Anti-Mouse | Biotin labeled goat anti-mouse | Biotin labeled rabbit anti-mouse | — | — | Biotin labeled rat anti-mouse | — | Biotin labeled horse anti-mouse |
| Anti-Human | Biotin labeled goat anti-human | Biotin labeled rabbit anti-human | Biotin labeled mouse anti-human | — | — | Biotin labeled bovine anti-human | — |
| Anti-Rabbit | Biotin labeled goat anti-rabbit | — | Biotin labeled mouse anti-rabbit | Biotin labeled donkey anti-rabbit | — | — | — |
| Anti-Rat | Biotin labeled goat anti-rat | Biotin labeled rabbit anti-rat | Biotin labeled mouse anti-rat | — | — | — | — |
| Anti-Goat | — | Biotin labeled rabbit anti-goat | Biotin labeled mouse anti-goat | — | — | — | — |
| Anti-Chicken | — | Biotin labeled rabbit anti-chicken | — | — | — | Biotin labeled bovine anti-chicken | — |
| Anti-Hamster | Biotin labeled goat anti-hamster | — | — | — | — | — | — |
| Anti-Sheep | — | Biotin labeled rabbit anti-sheep | — | — | — | — | — |
| Anti-Horse | Biotin labeled goat anti-horse | — | — | — | — | — | — |
Table 5. Biotinylated secondary antibodies for immunofluorescence applications
Table 6. Biotinylated secondary antibodies for flow cytometry
Biotinylated reagents for improved protein binding
Biotinylated reagents including biotin, biotin-X, and biotin-XX that are conjugated to secondary antibodies can be used to enhance subsequent binding to proteins such as streptavidin and avidin. Products that are denoted with an ‘X’ represent those that have a seven-atom aminohexanoyl spacer between biotin and the reactive carboxylic acid. This spacer helps to enhance binding to the biotin-binding pockets of avidin and streptavidin. DSB-X Biotin reagent also utilizes a seven-atom spacer to increase binding to streptavidin, avidin, or other biotin-binding proteins. However, DSB-X biotin conjugates can be reversibly bound to streptavidin or avidin, leaving the molecule of interest unchanged.
See all streptavidin/biotin binding protein conjugates for imaging or flow cytometry
Find Streptavidin Dynabeads for purification of protein or isolation of cells
Learn more about the Endogenous Biotin-Blocking Kit
Tyramide SuperBoost technology for use with biotin labeled secondary antibodies
Tyramide SuperBoost Signal Amplification is a highly sensitive method for the detection of low-abundance targets in multiplexable fluorescent IHC/ICC/ISH experiments. SuperBoost kits combine the brightness of Alexa Fluor dyes with the enhancement of tyramide signal amplification to produce a superior signal. For use with biotin conjugated antibodies use one of the Alexa Fluor Tyramide SuperBoost kits with streptavidin in table X below.
Find all Alexa Fluor streptavidin and avidin conjugates for biotin binding
Amplify the signal of your biotin labeled antibody using the Biotin XX Tyramide SuperBoost Kit, Streptavidin (Cat. No. B40931). This specific kit contains biotin XX tyramide with HRP-conjugated streptavidin, which is used prior to detection and signal amplification with an Alexa Fluor-conjugated streptavidin molecule. The table below provides various Alexa Fluor-streptavidin conjugates for use with Biotin XX Tyramide SuperBoost Kit, Streptavidin.
Figure 1. ATP Synthase detection with Biotin XX Tyramide SuperBoost Kit. HeLa cells were fixed and permeabilized with Image-iT Fixation/Permeabilization Kit (Cat. No R37602). For ATP synthase detection, cells were labeled with anti-ATP Synthase primary antibody and a biotin-conjugated secondary antibody. Biotin signal was enhanced using Biotin XX Tyramide SuperBoost Kit – Streptavidin (Cat. No B40931) and detected with Alexa Fluor 488 Streptavidin (Cat. No. S11223). For best antifade protection, cover slip was mounted with ProLong Diamond Antifade Mountant (Cat. No. P36961).