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DNA Phosphoramidites |
Thermo Scientific DNA phosphoramidites are built to meet the varying needs of oligonucleotide manufacturers for the development of therapeutic, diagnostic, and research applications. We offer TheraPure, standard, and fast deprotect phosphoramidites.
Our DNA phosphoramidites are supported by:
†May not be applicable in all regions.
Processes are monitored from material procurement through final QC and product release for batch-to-batch reproducibility. Purity of each phosphoramidite lot is assessed by HPLC-MS, GC, 31P NMR, as well as Karl Fischer oven titration. Our rigorously controlled manufacturing processes help minimize levels of reactive P(III) impurities which could be incorporated into the oligonucleotide during synthesis and result in the accumulation of by-products.
TheraPure DNA Phosphoramidite highlights
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dA(Bz) CEP | dC(Bz) CEP | dG(iBu) CEP | dT CEP | |
---|---|---|---|---|
Cat. No. | 27-2030 | 27-2032 | 27-2034 | 27-2036 |
Formula | C47H52N7O7P | C46H52N5O8P | C44H54N7O8P | C40H49N4O8P |
Molecular weight | 857.95 g/mol | 833.93 g/mol | 839.92 g/mol | 744.82 g/mol |
CAS # | 98796-53-3 | 102212-98-6 | 93183-15-4 | 98796-51-1 |
HPLC purity* | ≥ 99% | |||
31P NMR purity* | ≥ 99% | |||
Solution Clarity | Free from undissolved particulate at 0.2M solution in ACN | |||
Appearance | White to pale yellow powder | |||
Residual Solvent | ≤ 3.0% total % by weight | |||
Shipping/Storage condition | Ambient / –20 °C | |||
Water content | ≤ 0.3% | |||
Material Origin | Non-animal source (no TSE/BSE risk) | |||
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* For more specification details, contact us.
Traditional base protection methods are used to ensure quality and reliability for routine oligonucleotide synthesis.
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dA(Bz) CEP | dC(Bz) CEP | dG(iBu) CEP | dT CEP | |
---|---|---|---|---|
Cat. No. | 27-1730 | 27-1732* | 27-1734* | 27-1736 |
Formula | C47H52N7O7P | C46H52N5O8P | C44H54N7O8P | C40H49N4O8P |
Molecular weight | 857.95 g/mol | 833.93 g/mol | 839.92 g/mol | 744.82 g/mol |
CAS # | 98796-53-3 | 102212-98-6 | 93183-15-4 | 98796-51-1 |
HPLC purity | ≥ 98.0% | |||
31P NMR purity | ≥ 98.0% | |||
Water content (KF Method) | ≤ 0.3% | |||
Shipping / Storage condition | Ambient / –20 °C | |||
Solution Clarity | Free from undissolved particulate at 0.2M solution in ACN | |||
Material Origin | Non-animal source (no TSE/BSE risk) | |||
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* For more specification details, contact us.
Fast Deprotect DMF-dG, iPrPAC-dG, PAC-dA, Ac-dC, and iBu-dC phosphoramidites are ideal for applications requiring mild deprotection to preserve the integrity of oligonucleotides containing sensitive dyes, labels, or other modifications, which may be unstable with prolonged exposure to strong alkaline conditions. Fast Deprotect phosphoramidites permit the removal of base protecting groups under mild or accelerated conditions without the need for special reagents.
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iPrPAC-dG CEP | PAC-dA CEP | Ac-dC CEP | DMF-dG CEP | IBU-dC CEP | |
---|---|---|---|---|---|
Cat. No. (TheraPure phosphoramidites) | N/A | N/A | 27-2027 | N/A | N/A |
Cat. No. (standard) | 27-1726 | 27-1723 | 29-1727 | 27-1737 | 27-1725 |
Formula | C51H60N7O9P | C48H54N7O8P | C41H50N5O8P | C43H53N8O7P | C43H54N5O8P |
Molecular weight | 946.05 g/mol | 887.96 g/mol | 771.8 g/mol | 824.9 g/mol | 839.92g/mol |
CAS # | 150065-82-0 | 110543-74-3 | 154110-40-4 | 330628-04-1 | 110522-84-4 |
HPLC purity |(TheraPure)* | ≥ 98.0% | ≥ 98.0% | ≥ 98% | (≥ 99%) | ≥ 98.0% | ≥ 98.0% |
31P NMR purity | (TheraPure)* | ≥ 98.0% | ≥ 98.0% | ≥ 98% | (≥ 99%) | ≥ 98.0% | ≥ 98.0% |
Shipping / Storage condition | Ambient / –20 °C | ||||
Material Origin | Non-animal source (no TSE/BSE risk) | ||||
Request a quote |
* For more specification details, contact us.
仅供科研使用,不可用于诊断目的。