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RNA Phosphoramidites |
Thermo Scientific RNA phosphoramidites are built to meet the varying needs of oligonucleotide manufacturers for the development of therapeutic, diagnostic, and research applications. We offer TheraPure, standard, and fast deprotect phosphoramidites.
Our RNA phosphoramidites are supported by:
Processes are monitored from material procurement through final QC and product release for batch-to-batch reproducibility. Purity of each phosphoramidite lot is assessed by HPLC-MS, GC, 31P NMR, as well as Karl Fischer oven titration. Our rigorously controlled manufacturing processes help minimize levels of reactive P(III) impurities which could be incorporated into the oligonucleotide during synthesis and result in the accumulation of by-products.
Applied Biosystems/AKTA Synthesizer | Expedite Synthesizer | MerMade Synthesizer | Bulk | ||
---|---|---|---|---|---|
TheraPure phosphoramidites | Bz-rA | ✓ | ✓ | ✓ | ✓ |
Ac-rC | ✓ | ✓ | ✓ | ✓ | |
iBu-rG | ✓ | ✓ | ✓ | ✓ | |
rU | ✓ | ✓ | ✓ | ✓ | |
Standard phosphoramidites | Bz-rA | ✓ | |||
Ac-rC | ✓ | ||||
iBu-rG | ✓ | ||||
rU | ✓ | ||||
Fast deprotection (mild) | PAC-rA | ✓ | ✓ | ||
iPrPAC-rG | ✓ | ✓ | |||
Ac-rG | Contact us |
For items not listed above, flexible/bulk packaging, or custom services, please contact us ›
TheraPure RNA phosphoramidites were engineered specifically for oligonucleotide manufacturers requiring highly defined impurity profiles, strictly controlled processes with thorough documentation, and a rigorous process change control. With our in-house advanced analytical capabilities including LC-MS, GC, HPLC, 31P NMR and 1H NMR, meeting possible regulatory requirements should be achievable. Collaborative, customized specifications are always welcome for further discussion.
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Bz-rA CEP | iBu-rG CEP | Ac-rC CEP | rU CEP | |
---|---|---|---|---|
27-1903 | 27-1906 | 27-1805 | 27-1804 | |
Formula | C53H66N7O8PSi | C50H68N7O9PSi | C47H64N5O9PSi | C45H61N4O9PSi |
Molecular weight | 988.2 g/mol | 970.1 g/mol | 902.1 g/mol | 861.1 g/mol |
CAS # | 104992-55-4 | 147201-04-5 | 121058-88-6 | 118362-03-1 |
Appearance | Color intensity must not exceed Pantone #P1-4U or #P4-2U | |||
Solution Clarity | Free from undissolved particulate at 0.2M solution in ACN | |||
HPLC purity | ≥99% | |||
31P NMR purity | ≥99% | |||
Residual Solvent | ≤3.0% total % by weight solvent residue | |||
Shipping / Storage condition | Ambient / –20°C | |||
Water content | ≤0.3% by KF Method | |||
Material Origin | Non-animal source (no TSE/BSE risk) | |||
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Use these additional amidites with Ac-rC and U to mitigate the time for your oligonucleotide deprotection.
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Therapure PAC-rA CEP | Therapure iPrPAC-rG CEP | |
---|---|---|
Cat. No. | 27-1803 | 27-1806 |
Formula | C54H68N7O9PSi | C57H74N7O10PSi |
Molecular weight | 1018.2 g/mol | 1076.3 g/mol |
CAS # | 121058-86-4 | 956580-73-7 |
Solution Clarity | 0.2M solution in ACN is free from undissolved particulate | |
HPLC purity | ≥99% | |
31P NMR purity | ≥99% | |
Water content | ≤0.3% (KF Method) | |
Shipping / Storage condition | Ambient / –20°C | |
Material Origin | Non-animal source (no TSE/BSE risk) | |
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Traditional base protection methods are used to ensure quality and reliability for routine oligonucleotide synthesis.
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Bz-rA CEP | iBu-rG CEP | Ac-rC CEP | rU CEP | |
---|---|---|---|---|
Cat. No. | 27-1403 | 27-1406 | 27-1405 | 27-1404 |
Formula | C53H66N7O8PSi | C50H68N7O9PSi | C47H64N5O9PSi | C45H61N4O9PSi |
Molecular weight | 988.2 g/mol | 970.1 g/mol | 902.1 g/mol | 861.1 g/mol |
CAS # | 104992-55-4 | 147201-04-5 | 121058-88-6 | 118362-03-1 |
Appearance | Color intensity must not exceed Pantone #P1-4U or #P4-2U | |||
HPLC purity | ≥98.0% | |||
31P NMR purity | ≥98.0% | |||
Shipping / Storage condition | Ambient / –20°C | |||
Water content | ≤0.3% by KF Method | |||
Material Origin | Non-animal source (no TSE/BSE risk) | |||
Request a quote |
仅供科研使用,不可用于诊断目的。