Search Thermo Fisher Scientific
MicroRNA (miRNA) 和其他小 ncRNA 提供了天然基因沉默机制。它们在控制基因组调控方式以及环境和遗传因素传递或消除性状的方式方面发挥了核心作用。我们提供 TaqMan® 测定和其他专门用于 miRNA 和 ncRNA 研究的工具,从分离到发现、图谱分析、定量、验证以及功能分析,无所不包。了解有关 TaqMan® MicroRNA 测定及以下相关产品的更多信息。
15K+ microRNA 测定,基于 miRBase v21,包括相关 miRBase ID、登录号和别名。
了解 miRBase 术语、归一化结果、在线搜索和订购等。
下载将多达96种 microRNA RT 引物合并在一起用于多通路逆转录的实验方案。
MicroRNA 分析:Life Technologies 从发现到功能分析的解决方案
探索我们从发现到功能分析的 microRNA 分析解决方案。下载 microRNA 资源指南,了解创新的 microRNA 研究工具。
您想要哪种规格的测定? | 您正在开展哪种类型的研究? | 使用此 TaqMan® 测定/阵列 |
---|---|---|
单管 | 靶标 microRNA 分析 | |
Pri-miRNA(人/啮齿动物)分析 | ||
长非编码 RNA(人/啮齿动物)分析 | ||
以上任何一种,需要定制设计测定 | ||
384孔微流控卡 | 使用有限的样本或材料(人/啮齿动物)全面覆盖较为相关的 microRNA (miRBase v20) | TaqMan® 阵列 MicroRNA 卡 |
对所选 microRNA 进行图谱分析(使用预设计测定) | 定制 TaqMan® 阵列 MicroRNA 卡 | |
OpenArray® 板 | 大规模图谱分析(人/啮齿动物) | TaqMan® OpenArray® MicroRNA 检测板 |
荷兰 Erasmus 医学中心的 Leendert H. J. Looijenga 博士讨论了他以 DNA、mRNA、miRNA 和蛋白水平为重点、旨在发现癌症发展早期变化的研究。了解 Looijenga 博士如何使用 Life Technologies™ 产品检测体液中的靶标 miRNA。
Chang KH et al.(2010) MicroRNA expression profiling to identify and validate reference genes for relative quantification in colorectal cancer.BMC Cancer 10:173; doi: 10.1186/1471-2407-10-17.
Das S et al.(2013) Modulation of neuroblastoma disease pathogenesis by an extensive network of epigenetically regulated microRNAs.Oncogene 32(24):2927-2936; doi: 10.1038/onc.2012.
Di Leva G et al.(2013) Estrogen mediated-activation of miR-191/425 cluster modulates tumorigenicity of breast cancer cells depending on estrogen receptor status.PLoS Genet 9(3); doi: 10.1371/journal.pgen.1003311.
Francia S et al.(2012) Site-specific DICER and DROSHA RNA products control the DNA damage response.Nature 488(7410): 231–235; doi: 10.1038/nature11179.
Havelange V et al.(2011) Functional implications of microRNAs in acute myeloid leukemia by integrating microRNA and mRNA expression profiling.Cancer 117(20), doi: 10.1002/cncr.26096.
Hammell CM, Karp X, Ambros V (2009) A feedback circuit involving let-7-family miRNAs and DAF-12 integrates environmental signals and developmental timing in Caenorhabditis elegans.Proc Natl Acad Sci U S A 106(44): 18668–18673; doi: 10.1073/pnas.0908131106.
Hennessey PT et al.(2012) Serum microRNA biomarkers for detection of non-small cell lung cancer.PLoS One 7(2); doi: 10.1371/journal.pone.0032307.
Hudson RS et al.(2012) MicroRNA-1 is a candidate tumor suppressor and prognostic marker in human prostate cancer.Nucleic Acids Res 40(8): 3689–3703; doi: 10.1093/nar/gkr1222.
Karp X et al.(2011) Effect of life history on microRNA expression during C. elegans development.RNA 17(4): 639–651; doi: 10.1261/rna.2310111. 17(4): 639–651. doi: 10.1261/rna.2310111.Epub 2011 Feb 22.
Langevin SM et al.(2011) MicroRNA-137 promoter methylation is associated with poorer overall survival in patients with squamous cell carcinoma of the head and neck.Cancer 117(7): 1454–1462; doi: 10.1002/cncr.25689.
Nazari-Jahantigh M et al.(2012) MicroRNA-155 promotes atherosclerosis by repressing Bcl6 in macrophages.J Clin Invest 122(11): 4190–4202; doi: 10.1172/JCI61716.Epub 2012 Oct 8.
Patterson EE et al.(2011) MicroRNA profiling of adrenocortical tumors reveals miR-483 as a marker of malignancy.Cancer 117(8): 1630–1639; doi: 10.1002/cncr.25724.
Romano G et al.(2012) MiR-494 is regulated by ERK1/2 and modulates TRAIL-induced apoptosis in non–small-cell lung cancer through BIM down-regulation.Proc Natl Acad Sci U S A 109(41): 16570–16575; doi: 10.1073/pnas.1207917109.
Sheinerman KS et al.(2012) Plasma microRNA biomarkers for detection of mild cognitive impairment.Aging 4(9): 590–605.
van Schooneveld E et al.(2012) Expression profiling of cancerous and normal breast tissues identifies microRNAs that are differentially expressed in serum from patients with (metastatic) breast cancer and healthy volunteers.Breast Cancer Res.14(1): R34; doi: 10.1186/bcr3127.
Su X et al.(2010) TAp63 suppresses metastasis through coordinate regulation of Dicer and miRNAs.Nature 467(7318): 986–990; doi: 10.1038/nature09459.
Tili E et al.(2011) Mutator activity induced by microRNA-155 (miR-155) links inflammation and cancer.Proc Natl Acad Sci U S A 108(12): 4908–4913; doi: 10.1073/pnas.
Wang W et al.(2012) Identification of deregulated miRNAs and their targets in hepatitis B virus-associated hepatocellular carcinomaWorld J Gastroenterol 18(38): 5442–5453; doi: 10.3748/wjg.v18.i38.5442.
Yoshizawa S et al.(2012) Downregulated plasma miR-92a levels have clinical impact on multiple myeloma and related disorders.Blood Cancer J 2(1): e53; doi: 10.1038/bcj.2011.51.
Yu J et al.(2012) MicroRNA alterations of pancreatic intraepithelial neoplasms (PanINs).Clin Cancer Res 18(4): 981–992; doi: 10.1158/1078-0432.CCR-11-2347.
Zisoulis DG, Kai ZS, Chang RK, Pasquinelli AE (2012) Auto-regulation of miRNA biogenesis by let-7 and Argonaute.Nature 486(7404): 541–544; doi: 10.1038/nature11134.
浏览下列选项卡,查看 TaqMan® MicroRNA 测定的应用文档、视频和实验方案。
仅供科研使用,不可用于诊断目的。