Gibco Essential 6 Medium

Together with Essential 8 Medium, Essential 6 Medium completes a defined, xeno-free PSC workflow by serving as a growth factor–free base for differentiation and reprogramming.

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Gibco PSC Media are manufactured according to cGMP for medical devices, 21 CFR Part 820

A versatile basal medium for differentiation and other PSC applications

Unlike other media used in PSC culture, Essential 6 Medium does not contain bFGF or TGFβ.

Differentiated NSCs grown in Gibco Essential 6 Medium
图1. Neural stem cell (NSC) differentiation using Essential 6 Medium. PSCs cultured in Essential 8 Medium on rhVTN-N were differentiated into NSCs using Essential 6 Medium as a base, in accordance to Lippmann ES, et al. Stem Cells (2014). (Data generated by Randolph Ashton, PhD and Nisha Iyer, PhD, University of Wisconsin, Madison.)
Differentiated cardiomyocytes grown in Gibco Essential 6 Medium
图2. Cardiomyocyte differentiation using Essential 6 Medium. PSCs were differentiated into cardiomyocytes using WNT and GSK3B inhibitors in Essential 6 Medium.  
Differentiated motor neurons grown in Gibco Essential 6 Medium and graph showing expression profiles indicative of motor neuron progenitors
图3. Motor neuron (MN) differentiation using Essential 6 Medium. Combinations of growth factors and small molecules were added sequentially to PSCs in Essential 6 Medium according to the protocol in Lippmann ES, et al. Stem Cell Reports (2015) in order to generate various types of motor neurons. The data reveal different Hox expression profiles indicative of regionalized motor neuron progenitors (pMN). (Data generated by Randolph Ashton, PhD and Nisha Iyer, PhD, University of Wisconsin, Madison.)

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