Essential 8 Medium—Superior Recovery During Adaptation

The Gibco Essential 8 Adaptation Kit includes Gibco Essential 8 Medium and recombinant human rhLaminin-521 (rhLaminin-521) for optimal survival during transition from a feeder-dependent to feeder-free culture system.

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Gibco PSC Media are manufactured according to cGMP for medical devices, 21 CFR Part 820

Confidently support cells during transition from feeder-based to feeder-free culture conditions

> When used in conjunction with Essential 8 Medium, rhLaminin-521 enables a trouble-free transition from feeder-based media to a xeno-free, feeder-free media system.

Optimum recovery of PSCs during transition to Gibco Essential 8 Medium in the absence of small molecule inhibitors

图1. Optimum recovery of PSCs during transition to Gibco Essential 8 Medium in the absence of small molecule inhibitors. An episomal CD34+-derived iPSC line was cultured on mouse irradiated feeders in KSR-based medium prior to transition to Essential 8 Medium.   Confluency of cultures was monitored using the IncuCyte ZOOM™ system post-passage (n=3 per condition).

Four panel stem cell imaging results showing human embryonic stem cells stained using SSEA4 marker (green), OCT4 marker (red), DAPI (blue), and overlay of all three images.

图2. Human ESCs maintain expression of self-renewal factors.

Complete media system

The following components are recommended to create a complete media system for routine expansion and maintenance of PSCs with Essential 8 Medium.

Essential 8 Adaptation Kit Protocol

基质

Passaging reagent

相关资源

仅供科研使用，不可用于诊断目的。