Labeled Glycan Workflow

Released glycans have no chromophore and a poor response with conventional UV detection. They can be labeled with fluorescent and/or mass spectrometric tags prior to high sensitivity analysis by fluorescence detection, typically using 2-aminobenzamide (2-AB).

Thermo Scientific aminoxyTMT™ (Tandem Mass Tag™) reagents can also be used for labeling released glycans. This enables multiplexing experiments to be performed for quantitation of glycans by mass spectrometry.

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Get industry leading, ultra-high resolution of labeled and native glycans for structural characterization and quantification. Our range of glycan HPLC and UHPLC columns, includes:

• Thermo Scientific GlycanPac™ AXR1 LC columns. This high-performance, silica based chromatography column achieves mixed mode separation using a combination of charge, isomer structure, and size. Now there's a column with unique selectivity to analyze biologically important glycans.
• For glycans that contain ionizable groups, a mixed mode HILIC/anion exchange column like the Thermo Scientific GlycanPac™ AXH-1 column demonstrates a complementary solution for the analysis of charged glycans, with separation based on charge in addition to hydrophilicity.
• Hydrophilic interaction liquid chromatography (HILIC) features increased retention of polar species and has been shown to give good retention of  oligosaccharides. The Thermo Scientific Accucore 150-Amide-HILIC HPLC phase is designed for the separation of hydrophilic biomolecules. 

The new Thermo Scientific Vanquish Flex UHPLC delivers confidence, with a fully bio-compatible flow path and proven compliance. Achieve unprecedented glycan hplc performance in retention time stability, sensitivity and separation efficiency with the widest range of column chemistries for biotherapeutic protein characterization. Demand versatility through seamless integration with market-leading mass spectrometry, fluorescence and charged aerosol detection. Gain operational simplicity with easy, freely available, one-click workflows via AppsLab and tool-free Thermo Scientific Viper fittings.

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Vanquish UHPLC System: Advanced Detectors for Biopharma Characterization

Speed the characterization of complex glycans using SimGlycan™ software from PREMIER Biosoft. SimGlycan software processes and interprets the multi-stage/sequential (MSn) data produced by Thermo Scientific™ Mass Spectrometers, a capability essential to determining glycan heterogeneity and isomeric forms. The software automatically matches experimental mass spectra against a comprehensive database and generates a scored list of candidate structures. More relevant information about the proposed structure, including the glycan class, pathway and enzyme, is provided via interactive links. 

Streamline your laboratory workflow using Thermo Scientific Chromeleon 7.3 Chromatography Data System (CDS). This software delivers superior instrument control, automation, and data processing for compliant GxP biopharmaceutical manufacturing and QA/QC environments.

Run your routine UHPLC, or LC-MS released glycan analyses in an enterprise environment—from method creation to final reporting.

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One mass spectrometer for glycan and intact glycoform characterization. The Thermo Scientific Orbitrap Exploris 240 Mass Spectrometer of Orbitrap-based mass spectrometers add superior denatured and native MS intact analysis and subunit top/middle-down analysis capabilities to the most powerful benchtop peptide mapping instruments available.

When combined with Thermo Scientific BioPharma Finder software it provides a complete integrated hardware and software solution for biotherapeutic characterization.

The Thermo Scientific Orbitrap Eclipse Tribrid mass spectrometer is designed to surpasses challenges in quantitation using Thermo Scientific aminoxyTMT (TM) and low level glycan analysis with new innovations that deliver the ultimate flexibility to expand experimental scope, and with built-in intelligence to ensure the highest data quality and confidence. One system provides maximum insights, so you productively go beyond today’s discovery.