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LED-based fluorescence as well as brightfield illumination and confocal capabilities
Catalog numbers: HCSDCX7LEDPRO
The Thermo Scientific CellInsight CX7 LED Pro High-Content Screening Platform is an LED-based platform that offers a choice of imaging modes to extract the information you need from your samples. Both well-by-well and channel-by-channel, you can select the right modes to read your sample—with the resolution and dynamic range that results from the high performance of the optical train and sensitive camera. Use the entire fluorescence spectrum to optimize your assay and select either widefield or confocal optics for any channel. Browse the features, specifications, videos, sample data, and reagent selection guide sections.
Features
In addition to the common features of all high-content screening platforms, the CellInsight CX7 LED Pro platform offers:
Next generation sCMOS camera
The CX7 LED Pro has been upgraded with a best-in-class sCMOS camera, offering greater than 95% peak quantum efficiency and low background 1.0e-ready noise. This near-perfect quantum efficiency further accelerates your high-throughput performance thanks to reduced exposure times across all wavelengths. Boast your experiment’s assay window, with up to 65,536 shades of gray detection while not sacrificing background noise thanks to the -20˚C cooling.
Introducing Olympus X-line objectives to the CX7 LED Pro
The CX7 LED Pro now offers Olympus X-line objectives to further improve the instruments imaging quality and assay performance. Publication quality imaging is now standard using the new CX7 LED Pro platform.
Live-cell experiment optimization
The CX7 LED Pro light has been optimized for live-cell experiments. Laser illumination provides flatter and brighter images with low background.
Confocal imaging mode
CrEST™ spinning-disk confocal technology with dual 40 or 70 micron pinhole size. The CrEST confocal is engineered directly into the optical path to provide high-resolution confocal imaging capabilities.
Brightfield imaging mode
Using an LED array for RGB and amber illumination, this mode allows users to make colorimetric absorbance measurements of histology samples with classic stains like H&E.
Widefield imaging mode
The 7-color light engine reduces switching times and intensity fluctuations to reduce scan times and boost quantitative performance.
Automation
The CX7 LED Pro is configured for fully automated plate handling and scanning.
Multiplexing
Multiplex your colorimetric absorbance data with fluorescence measurements, offering more possibilities for verification and correlation.
Specifications
Category |
Attribute |
Description |
Optics |
Camera |
Photometrics High-Resolution Fluorescent Camera:
|
|
Light source |
LED, solid-state 7-color light engine used with provided filter sets offers the following excitation/emission capabilities (Ex/Em, in nm):
|
|
Objectives |
Standard (Olympus™ objectives):
Optional (Olympus objectives):
|
|
Image compatibility |
JPG, BMP, GIF, PNG, TIF, C01, DIB |
Physical characteristics |
Dimensions |
20 in x 32 in x 18 in (50.8 cm x 81.3 cm x 45.7 cm) |
|
Weight |
68 kg (150 lb) |
System |
Data management |
Compatible with Thermo Scientific Store Image and Database Management Software |
|
Software |
Thermo Scientific HCS Studio 5.0 Cell Analysis Software with Cell-Painting Bioapplication |
|
PC |
Windows 10 Professional 64-Bit PC with 32GB (4x8GB) RAM, Intel® Xeon® Processor 3.7GHz Turbo, RAID I Controller with 1.8TB Hard Drive and 256 Boot Drive. Includes Dell 24" High Resolution Widescreen Monitor or equivalent, keyboard and mouse |
|
Wattage |
300 W |
Videos and demos
Sample data
HeLa cells imaged using the CellInsight CX7 platform
HeLa cell image acquired using the Thermo Scientific CellInsight CX7 platform and stained with Invitrogen HCS NuclearMask Blue (blue), Invitrogen MitoTracker Orange (green), and Invitrogen Alexa Fluor 647 Phalloidin (red).
A549 cells imaged using the CellInsight CX7 platform
A549 cell image acquired using the Thermo Scientific CellInsight CX7 platform and stained with Invitrogen HCS CellMask Blue (blue), Invitrogen Alexa Fluor 488 Phalloidin (green), and Invitrogen Alexa Fluor 750 goat anti–mouse IgG secondary antibody (pink).
Human breast cancer tissue section imaged using the CellInsight CX7 platform
Three-color brightfield image of fixed human breast cancer tissue stained with KI67-DAB and counterstained with hematoxylin and eosin and imaged with the Thermo Scientific CellInsight CX7 platform.
-600-dpi.png "HeLa 488 tubulin confocal led")
HeLa cells imaged using the CellInsight CX7 LED instrument
HeLa cells labeled with tubulin antibody followed by Invitrogen Alexa Fluor Plus 488 antibody and Hoechst 34580 staining. Imaging was performed on the CellInsight CX7 LED instrument using a 60x objective and confocal mode.
600-(1)-600-dpi-nucleus.png "HeLa 650 tubulin 60x LED")
HeLa cells imaged using the CellInsight CX7 LED instrument
HeLa cells labeled with tubulin antibody followed by Invitrogen Alexa Fluor Plus 647 antibody and Hoechst 34580 staining. Imaging was performed on the CellInsight CX7 LED instrument using a 60x objective and confocal mode.
600-(1)-600dpi.png "HeLa cells imaged using CX7 LED")
HeLa cells imaged using the CellInsight CX7 LED instrument
HeLa cells labeled with ATP synthase V antibody followed by Alexa Fluor Plus 488 antibody and Hoechst 34580 staining. Imaging was performed on the CellInsight CX7 LED instrument using a 60x objective and confocal mode.
Reagent selection guide
The Thermo Scientific CellInsight CX7 LED Pro high-content instrument allows you to take advantage of the entire fluorescence spectrum to optimize your assay—and multiplex your components to ask more in-depth biological questions. Learn more about reagents for cell viability, proliferation, and function or reagents to determine cell structure. For more specific applications using assays and high-content platforms, check out Assay Selection.
For Research Use Only. Not for use in diagnostic procedures.