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Cat. No. | Coelenterazine Analog | Em * (nm) | RLC † | Relative Intensity ‡ | Half-Rise Time (msec) § |
---|---|---|---|---|---|
C2944 | native | 466 | 1.00 | 1 | 6–30 |
C14260 | cp | 442 | 0.63 | 28 | 2–5 |
C6779 | f | 472 | 0.80 | 20 | 6–30 |
C6780 | h | 466 | 0.75 | 16 | 6–30 |
* Emission maxima. † Relative luminescence capacity = total time-integrated emission of aequorin in saturating Ca2+ relative to native aequorin = 1.0. ‡ Relative intensity at 100 nM Ca2+. § Half-rise time = time for the luminescence signal to reach 50% of the maximum after addition of 1 mM Ca2+ to a standard of aequorin reconstituted with the coelenterazine analog of interest. All data are from Cell Calcium (1993) 14:373. |
For Research Use Only. Not for use in diagnostic procedures.