Table 9.3 Molecular Probes fluorescent and luminescent protein blot stains.

Dye Name (Cat. No.) Ex/Em * Major Applications Features
SYPRO Ruby protein blot stain (S11791)280, 450/618Mass spectrometry, microsequencing, counterstain for blot-based detection techniques (nitrocellulose or PVDF membranes)
  • High sensitivity (1–2 ng/band; comparable to colloidal gold staining)
  • Reversible
BODIPY FL-X succinimidyl ester (D6102) †365, 505/575Counterstain for blot-based detection techniques (PVDF membranes)
  • Very good sensitivity (4–8 ng/band)
  • Permanent, covalent staining for multicolor techniques
BODIPY TR-X succinimidyl ester (D6116) ‡300, 590/615Counterstain for blot-based detection techniques (PVDF membranes)
  • Very good sensitivity (4–8 ng/band)
  • Permanent, covalent staining for multicolor techniques
* Excitation (Ex) and emission (Em) maxima, in nm. For maximum sensitivity, use excitation sources and optical filters matched to these values. † Available as a stand-alone reagent (D6102, BODIPY Dye Series—Section 1.4). ‡ Available as a stand-alone reagent (D6116, BODIPY Dye Series—Section 1.4).

For Research Use Only. Not for use in diagnostic procedures.