R<0> values for QSY and dabcyl quenchers—Table 1.11

Donor	Acceptor
Donor	QSY 35	dabcyl	QSY 7 and QSY 9	QSY 21
Alexa Fluor 350	47	50
Alexa Fluor 488	44	49	64
Alexa Fluor 546	25	29	67
Alexa Fluor 555			45
Alexa Fluor 568			56	75
Alexa Fluor 594				77
Alexa Fluor 647				69
R₀ values in angstroms (Å) represent the distance at which fluorescence resonance energy transfer from the donor dye to the acceptor dye is 50% efficient (Förster radius). Values were calculated from spectroscopic data as outlined (see Fluorescence Resonance Energy Transfer (FRET)—Note 1.2).

For Research Use Only. Not for use in diagnostic procedures.

R0 Values for QSY and Dabcyl Quenchers—Table 1.11