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Esterase Substrate (Cat. No.) | Properties in Cells | pKa of Product * |
---|---|---|
BCECF AM (B1150, B1170, B3051) |
| 7.0 |
Calcein AM (C1430, C3099, C3100MP) |
| 5 |
Carboxyeosin diacetate, succinimidyl ester (CEDA, SE; C22803; Assays for Cell Enumeration, Cell Proliferation and Cell Cycle—Section 15.4) |
| <5 † |
Carboxy-2',7'-dichlorofluorescein diacetate (carboxy-DCFDA, C369) |
| 4.8 |
Carboxyfluorescein diacetate (5(6)-CFDA, C195) |
| 6.4 |
Carboxyfluorescein diacetate, acetoxymethyl ester (5-CFDA, AM; C1354) |
| 6.4 |
Carboxyfluorescein diacetate, succinimidyl ester (5(6)-CFDA, SE; C1157; Assays for Cell Enumeration, Cell Proliferation and Cell Cycle—Section 15.4) |
| 6.4 † |
CellTracker Green CMFDA (CMFDA; C2925, C7025) |
| 6.4 † |
Chloromethyl SNARF-1, acetate (C6826) |
| 7.5 † |
Fluorescein diacetate (F1303) |
| 6.4 |
Oregon Green 488 carboxylic acid diacetate (carboxy-F2FDA, O6151) |
| 4.7 |
* Approximate pKa values in aqueous solvents. The actual pKa of the indicator will vary somewhat depending upon experimental conditions. † pKa of the unconjugated hydrolysis product; after conjugation to an intracellular amine or thiol, the actual pKa value may be different. |
For Research Use Only. Not for use in diagnostic procedures.