Reactive oxygen species—Table 18.1

Table 18.1 Reactive oxygen species.

Reactive Oxygen Species	Structure	Detection Reagents
Hydrogen peroxide	H₂O₂	Carboxy-H₂DCFDA (C400) CellROX Orange reagent (C10443, C10493) CM-H₂DCFDA (C6827) Dihydrocalcein AM (D23805) Dihydrorhodamine 123 (D632, D23806) Dihydrorhodamine 6G (D633) H₂DCFDA (D399) Lucigenin (L6868) Luminol (L8455) RedoxSensor Red CC-1 (R14060)
Hydroxyl radical *	HO•	3'-(p-Aminophenyl) fluorescein (APF, A36003) 3'-(p-Hydroxyphenyl) fluorescein (HPF, H36004) CellROX Green reagent (C10444, C10492) CellROX Orange reagent (C10443, C10493) CellROX Deep Red reagent (C10422, C10491) CM-H₂DCFDA (C6827) Proxyl fluorescamine (C7924) TEMPO-9-AC (A7923)
Hypochlorous acid	HOCl	Aminophenyl fluorescein (APF, A36003) Dihydrorhodamine 123 (D632, D23806) Luminol (L8455)
Nitric oxide	NO	CellROX Orange reagent (C10443, C10493) DAF-FM (D23841) DAF-FM diacetate (D23842, D23844) DAA (D23840) 2,3-Diaminonaphthalene (D7918) Luminol (L8455)
Peroxyl radical, including both alkylperoxyl and hydroperoxyl radicals (wherein R = H)	ROO•	BODIPY FL EDA (D2390) BODIPY 665/676 (B3932) H₂DCFDA (D399) Carboxy-H₂DCFDA (C400) CM-H₂DCFDA (C6827) DPPP (D7894) Luminol (L8455) cis-Parinaric acid (P36005) RedoxSensor Red CC-1 (R14060)
Peroxynitrite anion †	ONOO^–	3'-(p-Aminophenyl) fluorescein (APF, A36003) 3'-(p-Hydroxyphenyl) fluorescein (HPF, H36004) CellROX Orange reagent (C10443, C10493) H₂DCFDA (D399) Carboxy-H₂DCFDA (C400) CM-H₂DCFDA (C6827) Coelenterazine (C2944) Dihydrorhodamine 123 (D632, D23806) Dihydrorhodamine 6G (D633) Luminol (L8455)
Singlet oxygen ‡	¹O₂	Singlet Oxygen Sensor Green reagent (S36002) trans-1-(2'-methoxyvinyl)pyrene (M7913)
Superoxide anion	•O₂^–	CellROX Green reagent (C10444, C10492) CellROX Orange reagent (C10443, C10493) CellROX Deep Red reagent (C10422, C10491) Coelenterazine (C2944) Dihydroethidium (D1168, D11347, D23107) Fc OxyBURST Green assay reagent (F2902) OxyBURST Green H₂DCFDA SE (D2935) OxyBURST Green H₂HFF BSA (O13291) Lucigenin (L6868) Luminol (L8455) MCLA (M23800) MTT (M6494) NBT (N6495) RedoxSensor Red CC-1 (R14060) TEMPO-9-AC (A7923) XTT (X6493)
* Hydroxyl radicals can also be photosensitized by malachite green isothiocyanate (M689) or generated by a N-(1,10-phenanthrolin-5-yl)iodoacetamide (P6879) metal–ligand complex. † 3-Nitrotyrosine, a product of this potent nitrating reagent, can be detected with an anti-nitrotyrosine antibody (A21285). ‡ Singlet oxygen can also be photosensitized by hypericin (H7476), rose bengal diacetate (R14000) and merocyanine 540 (M24571).

For Research Use Only. Not for use in diagnostic procedures.

Table 18.1 Reactive oxygen species.