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Donor |
Acceptor
| |||||
---|---|---|---|---|---|---|
Alexa Fluor 488 | Alexa Fluor 546 | Alexa Fluor 555 | Alexa Fluor 568 | Alexa Fluor 594 | Alexa Fluor 647 | |
Alexa Fluor 350 | 50 | |||||
Alexa Fluor 488 | NA | 64 | 70 | 62 | 60 | 56 |
Alexa Fluor 546 | NA | 70 | 71 | 74 | ||
Alexa Fluor 555 | NA | 47 | 51 | |||
Alexa Fluor 568 | NA | 82 | ||||
Alexa Fluor 594 | NA | 85 | ||||
Alexa Fluor 647 | NA | |||||
R0 values in angstroms (Å) represent the distance at which fluorescence resonance energy transfer from the donor dye to the acceptor dye is 50% efficient (Förster radius). Values were calculated from spectroscopic data as outlined (Fluorescence Resonance Energy Transfer (FRET)—Note 1.2). NA = Not applicable. |
For Research Use Only. Not for use in diagnostic procedures.