Question 1

Why switch to a new film?

Accepted Answer

We have a culture of continuous improvement and innovation. Based on feedback from customers, we designed a bag portfolio using a consistent film across all sizes. We also achieved a new film superior in function to other commercially available films.

Question 2

When will I start to receive bags with the new film?

Accepted Answer

We will begin production in the new film starting in February, 2010. You may continue to receive product with the original film until our stock is depleted. We expect to have all product in the new Gibco PE film by mid year.

Question 3

What are the benefits of the PE film?

Accepted Answer

The commercially available film is a very clear, multi-layered single web film with a polyethylene contact surface that can be made into a wide range of bag sizes (250mL – 1,000L). In addition, the film is a market leader with exceptional gas barrier properties with very low extractable and leachable results.

Question 4

Does this mean there are changes to the formulations?

Accepted Answer

No. There is no change to the product formulation inside the bag and the new Gibco PE film will not affect media properties or effectiveness. All products are still manufactured at our Gibco production sites in Grand Island, NY or Paisley, Scotland.

Question 5

Will anything else change with the bags?

Accepted Answer

The shape and design of the existing bag will remain constant. All tubing and fittings of the universal bags will remain in place to offer a wide range of connection options. The outer package of the new bags has been improved to provide more support and structure for transportation and storage. Your bag will now ship in all brown, 100% recyclable, heavyweight corrugated box. We have also added some additional formulas as stocked catalog products. We will now sell Freestyle 293 and CD CHO in the universal bags with the Gibco® PE film.

Question 6

In what size package will my bags ship?

Accepted Answer

Each bag size will arrive in a specific heavyweight corrugated box designed for shipment 5L – 12” x 14.25” x 5.5” 10L – 13” x 23.5” 6.125” 20L – 15” x 24” x 6.125”

Question 7

What sized bags are currently available?

Accepted Answer

Large size bags are currently available with a continued release of additional sizes throughout the year. We plan to start filling select formulas in the 5, 10, and 20 liter bags starting in Q1 2010 and will have a rolling launch of all catalog offered formulas.

Question 8

Will you continue to support other films?

Accepted Answer

Yes. Although we feel that the Gibco PE film exceeds many of the technical properties of the other available films, we will continue to offer and support the other currently available films through custom orders.

Question 9

Will my previous custom configuration change to the new film?

Accepted Answer

No. All custom re-orders in the small bags will use the same film/bag as your original order. If you would like to switch to the new film, please let us know during the order process and we will accommodate your request.

Question 10

What will I have to do differently?

Accepted Answer

Nothing. The catalog numbers will remain the same and you will continue to get your orders as usual. Each formula may be available in the new film at different times, but the change will occur automatically with no action needed on your part.

Question 11

Can I find out what products are on stability and specifically if my product is?

Accepted Answer

We can share with you our list of products on stability. However, we do not put all products on stability – only representative formulations.

Question 12

What does this change ultimately mean to me?

Accepted Answer

Gas transmission rates: The Gibco PE film has superior transmission rates when compared to other commercially available films. Bag size flexibility: You will now be able to receive a wider range of bag sizes using the same film which makes scale up easier for you. The ability to scale up will allow you to receive different sized bags without you having to validate multiple films. Documentation: The documentation for the Gibco PE film exceeds many others. It includes USP Class VI, leachable, extractable, gas permeation, biological and physical property to name a few. Clarity: The clarity of this film is much better than multi-webbed films and most single web films. Contact layer: The Gibco PE film has a polyethylene contact layer.

物理性质	尺寸	数值（γ 灭菌前后）*	程序
雾度	%	5 / 5	ASTM D-1003
透光度	%	98 / 98	ASTM D-1003
透射率	%	93 / 93	ASTM D-1003
断裂时的抗张强度	MPa	14 / 13	ASTM D-882
断裂时的拉伸率	%	280 / 300	ASTM D-882
弹性模量	MPa	370 / 350	ASTM D-882
在低温下断裂	ºC	低于 -45 / 低于 -45	ISO 8570
密度	g/cm³	0.9	ASTM D-792
水蒸气传输速率**	g/（m².天）	0.35 / 0.32	ASTM F-1249
氧气渗透性**	cm³/（m².天.atm）	<0.05 / <0.05	ASTM D-3985
二氧化碳渗透性**	cm³/（m².天.atm）	<0.2 / <0.2	ASTM F-2476
膜厚度	0.325 mm
* γ 灭菌剂量 25 kGy（标记为 ** 的参数接收 50 kGy 除外）

检测	规格	结果
表征	通过	通过
鉴定（A-IR 分光光度法）	通过	通过
鉴定（B-二氧化钛）	通过（≤4%）	通过
C-二氧化钛	通过	通过
溶液 S1 的外观	通过	通过
酸碱度	≤1.5 mL 0.01 N NaOH ≤1.0 mL 0.01 N HCl	通过（0.60 mL）通过（0.40 mL）
紫外吸光度（220-340 nm）	≤0.20	通过（0.01）
还原物质	≤0.5 mL	通过（0.20 mL）
可提取铝	≤1 ppm	通过
可提取锆	0.0	≤ 0.1 ppm
可提取铬	≤0.05 ppm	通过
可提取重金属	≤2.5 ppm	通过
可提取钛	≤1 ppm	通过
可提取钒	≤0.1 ppm	通过
可提取锌	≤1 ppm	通过
可提取锆	≤0.1 ppm	通过
硫酸盐灰分	≤ 1%	通过（0.02%）
可溶于己烷的物质	不适用	不可行
抗氧化剂（最大3）	通过	通过
抗氧化剂	<0.3%	通过
酰胺和硬脂酸盐	通过	通过

检测	方法	规格	结果
非挥发性残留	USP <661>	≤15 mg	通过
炽灼残渣	USP <661>	≤5 mg	通过
重金属	USP <661>	<2 ppm	通过
缓冲容量	USP <661>	≤10 mL	通过

检测	方法	规格	结果
洗脱测试	USP <87>	通过	通过
系统进样检测	USP <88>	VI 级	通过
皮内试验	USP <88>	VI 级	通过
缓冲容量	USP <88>	VI 级	通过

细胞培养袋

使用细胞培养袋的原因

Gibco 细胞培养袋的特点和优势

灵活的设计

超低密度聚乙烯（PE）接触层

技术概要

符合性规格

生理化学规格

生物兼容性规格

气体传输速率

Gibco PE 薄膜结构

Gibco PE 薄膜 pH 稳定性

可定制

订购细胞培养袋中的培养基产品

Related products

		50 μm 聚乙烯 10 μm 粘性层 20 μm 乙基乙烯醇（气体阻隔） 10 μm 粘性层 230 μm 超低密度聚乙烯（接触层）
图2.Gibco PE 薄膜的结构