Single Stranded DNA Graph
许多应用都要求使用单链 DNA 模板。通过固定双链 PCR 产物来制备两个单链 DNA 片段可能是 Dynabeads M-280 链霉亲和素磁珠应用较广泛的方案。首先对两个 PCR 引物之一进行生物素化，生成 PCR 产物。将 PCR 产物固定到磁珠上，以分离生物素化和非生物素化链。洗脱条件可以是加热或碱变性。所有多余的引物、核苷酸和缓冲液组分均被去除，无需离心或沉淀。 

链霉亲和素偶联 Dynabeads 不会抑制任何酶活性。这使得可以直接在固相上进一步处理和操作磁珠结合 DNA。

固定和洗脱的单链 DNA 模板均可用于下游应用，如 MALDI-MS (1)、焦磷酸测序技术 (2) 和 SNP 分析 (3)，以及单链构象多态性 (SSCP)、固相 DNA 测序 (4,5)、DNA 芯片&微阵列、等位基因特异性延伸和引物延伸。单链 DNA 模板也可用于体外突变、核酸酶 S1 作图 (6)、探针制备和标记 (7)、消减杂交 (8,9,10) 以及多种其他分子技术。

部分参考文献

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