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LC3Bは、オートファジーの早い段階で細胞質からリクルートされ、隔離膜と結合します。この局在化は、オートファジー膜およびその過程をモニタリングするための一般的マーカーとしての役割を果たします。Molecular Probes®のツールにより以下のことが可能です:
|  U2OS細胞を Premo™ Autophagy Sensor LC3B-RFP (赤色) および CellLight® Tubulin-GFP (緑色)を用いて形質導入 |
Premo™ Autophagy Sensor Tandem LC3Bを用いて、酸性と中性のLC3B陽性小胞を区別することができます。
|  Premo™ Autophagy Sensor Tandem RFP-GFP-LC3B を使用し、酸性と中性のLC3B陽性小胞を区別 |
p62は、クリアランスされるユビキチン化タンパク質凝集体など、オートファジーによって分解される細胞質カーゴを輸送するレセプターです。p62タンパク質はユビキチンそしてLC3との結合能をもち、それによってオートファゴソームをターゲットし、ユビキチン化タンパク質のクリアランスを促進します。 Molecular Probes®のツールにより以下のことが可能です:
|  Premo™ Autophagy Sensor GFP-p62 Kit を用いた、微小管に沿ったp62陽性小胞の運動性のイメージング |
| Target | LC3B associates with the phagophore (nascent autophagosomal membrane) early in autophagy | ||
| Readout | Localization of LC3B by fluorescence imaging | ||
| Fluorescent label | GFP | RFP | Requires secondary detection |
| Standard filter set | FITC | TRITC | NA |
| Ex/Em (nm) | 485/520 | 555/584 | NA |
| Signal-to-noise ratio |  | | NA |
| Photostability | | | NA |
| Bibliography | Citations | Citations | |
| Multiplexing | Yes | Yes | |
| Live cells | Yes | No | |
| Fixed cells | No | Yes | |
| Fixable | Yes | Yes | |
| Format | 1 kit | 1 kit | 1 kit |
| Cat. No. | P36235 | P36236 | L10382 |
| Target | LC3B associates with the phagophore (nascent autophagosomal membrane) early in autophagy and pH decreases after fusion with the lysosome | |
| Readout | Tandem sensor to monitor LC3B progression from autophagosome to autolysosome | |
| Fluorescent label | GFP | RFP |
| Standard filter set | FITC | TRITC |
| Ex/Em (nm) | 485/520 | 555/584 |
| Signal-to-noise ratio | | |
| Photostability | | |
| Bibliography | Citations | |
| Multiplexing | Yes | |
| Live cells | Yes | |
| Fixed cells | No | |
| Fixable | No | |
| Format | 1 kit | |
| Cat. No. | P36239 | |
| Target | p62 binds to ubiquitin cargo and LC3 or its homologs | |
| Readout | Localization of p62 by fluorescence imaging | |
| Fluorescent label | GFP | RFP |
| Standard filter set | FITC | TRITC |
| Ex/Em (nm) | 485/520 | 555/584 |
| Signal-to-noise ratio | | |
| Photostability | | |
| Bibliography | Citations | |
| Multiplexing | Yes | |
| Live cells | Yes | |
| Fixed cells | No | |
| Fixable | Yes | |
| Format | 1 kit | 1 kit |
| Cat. No. | P36240 | P36241 |
| Target | LC3B associates with the phagophore (nascent autophagosomal membrane) early in autophagy | ||
| Readout | Localization of LC3B by fluorescence imaging | ||
| Fluorescent label | GFP | RFP | Requires secondary detection |
| Standard filter set | FITC | TRITC | NA |
| Ex/Em (nm) | 485/520 | 555/584 | NA |
| Signal-to-noise ratio | | | NA |
| Photostability | | | NA |
| Bibliography | Citations | Citations | |
| Multiplexing | Yes | Yes | |
| Live cells | Yes | No | |
| Fixed cells | No | Yes | |
| Fixable | Yes | Yes | |
| Format | 1 kit | 1 kit | 1 kit |
| Cat. No. | P36235 | P36236 | L10382 |
| Target | LC3B associates with the phagophore (nascent autophagosomal membrane) early in autophagy and pH decreases after fusion with the lysosome | |
| Readout | Tandem sensor to monitor LC3B progression from autophagosome to autolysosome | |
| Fluorescent label | GFP | RFP |
| Standard filter set | FITC | TRITC |
| Ex/Em (nm) | 485/520 | 555/584 |
| Signal-to-noise ratio | | |
| Photostability | | |
| Bibliography | Citations | |
| Multiplexing | Yes | |
| Live cells | Yes | |
| Fixed cells | No | |
| Fixable | No | |
| Format | 1 kit | |
| Cat. No. | P36239 | |
| Target | p62 binds to ubiquitin cargo and LC3 or its homologs | |
| Readout | Localization of p62 by fluorescence imaging | |
| Fluorescent label | GFP | RFP |
| Standard filter set | FITC | TRITC |
| Ex/Em (nm) | 485/520 | 555/584 |
| Signal-to-noise ratio | | |
| Photostability | | |
| Bibliography | Citations | |
| Multiplexing | Yes | |
| Live cells | Yes | |
| Fixed cells | No | |
| Fixable | Yes | |
| Format | 1 kit | 1 kit |
| Cat. No. | P36240 | P36241 |
お役立ち情報 | |
| Molecular Probes® Handbook BioProbes® Journal Article Technical Resources | Related Products |