Disease Modeling Resources | Thermo Fisher Scientific

We have collaborated with The Parkinson’s Institute in Sunnyvale, California, to demonstrate how to develop cellular models to identify drugs that may ameliorate the underlying processes of Parkinson’s disease (PD). In a series of white papers, we described three steps for creating PD cell models using Life Technologies tools for generation of NSCs, iPSCs, and editing the genome. We have collected a set of resources here to help enable your disease modeling research.

Protocols and user guides on this page	More resources on this page
Culture Engineering Differentiation Characterization	Webinars Seminars Scientific Posters Research Highlights

What assay?	Workflow step	What consumable?	Protocol or user guide
iPSC culture	Culture	KnockOut™ Serum Replacement	Feeder-Dependent Culture of Human Induced Pluripotent Stem Cells
iPSC culture	Culture	Essential 8™ Medium	Culturing Pluripotent Stem Cells (PSCs) in Essential 8™ Medium
iPSC culture	Culture	mouse embryonic fibroblasts	Culturing Induced Pluripotent Stem Cells (iPSCs) on MEF Conditioned Medium
hESC culture	Culture	mouse embryonic fibroblasts	Culturing Induced Pluripotent Stem Cells (hESCs) on MEF Conditioned Medium
NSC culture	Culture	Neural Expansion Medium	Expansion of Neural Stem Cells Using Neural Expansion Medium
NSC culture	Culture	StemPro® NSC SFM	Expansion of Neural Stem Cells Using StemPro® NSC SFM

What assay?	Workflow step	What instrument?	What consumable?	Protocol or user guide
iPSC generation	Engineer	EVOS® XL Imaging System	CytoTune®-iPS Sendai Reprogramming Kit	Reprogramming Fibroblasts with the CytoTune®-iPS Reprogramming Kit
iPSC reprogramming efficiency calculation	Engineer	EVOS® FLoid® Cell Imaging Station	CytoTune®-iPS Sendai Reprogramming Kit	Calculating Reprogramming Efficiency using CytoTune®-iPS Reprogramming Kit
Genomic cleavage	Engineer	Agarose gel electrophoresis	GeneArt® Genomic Cleavage Detection Kit	GeneArt® Genomic Cleavage Detection Kit - User Guide
SNP genotyping	Engineer	QuantStudio® 12K Flex Real-Time PCR System	Custom TaqMan® SNP Genotyping Assays	Custom TaqMan® SNP Genotyping Assays - Protocol
Targeted DNA double-stranded breakage via electroporation	Engineer	Neon® Transfection System	GeneArt® TALs Products and Services	GeneArt® Precision TALs - User Manual

What assay?	Workflow step	What instrument?	What consumable?	Protocol or user guide
iPSC characterization - EB formation immunostaining	Differentiate	EVOS® FLoid® Cell Imaging Station	antibodies	Creation of Embryoid Bodies from iPSCs
NSC generation	Differentiate	EVOS® XL Imaging System	PSC Neural Induction Medium	Induction of Neural Stem Cells Using Gibco® PSC Neural Induction Medium
NSC differentiation - DA neuron	Differentiate	EVOS® FLoid® Cell Imaging Station	50X B-27® Serum-Free Supplement, 100X N-2 Supplement	Differentiating Neural Stem Cells into Neurons
NSC differentiation - glial	Differentiate	EVOS® FLoid® Cell Imaging Station	50X B-27® Serum-Free Supplement, 100X N-2 Supplement	Differentiating Neural Stem Cells into Glial Cells

What assay?	Workflow step	What instrument?	What consumable?	Protocol or user guide
Search for additional mutations	Characterize	Ion PGM™ Sequencer	AmpliSeq™ Inherited Disease Panel	Ion Torrent Protocol Community
iPSC characterization - cytogenetic	Characterize	None	KaryoMAX® Giemsa Stain Solution	Giemsa Banding (“GTG” Banding)
iPSC characterization - pluripotent markers immunostaining	Characterize	EVOS® FLoid® Cell Imaging Station	antibodies	TRA 1-60 antibody TRA-1-81 antibody SSEA4 Antibody Hoechst 33342 Oct4 Antibody Goat Anti–Mouse IgG Antibodies
iPSC characterization - pluripotent markers immunostaining	Characterize	EVOS® FLoid® Cell Imaging Station	PSC 4-Marker Immunocytochemistry Kit PSC (OCT4, SSEA4) Immunocytochemistry Kit PSC (SOX2, TRA-1-60) Immunocytochemistry Kit	PSC Immunocytochemistry Kits (4-Marker) (OCT4, SSEA4) (SOX2, TRA-1-60) - User Manual
iPSC characterization - pluripotent markers flow analysis	Characterize	Attune® Acoustic Focusing Cytometer	antibodies	Attune® Acoustic Focusing Cytometer
iPSC characterization - gene expression profile	Characterize	QuantStudio™ 12K Flex Real-Time PCR System	TaqMan® OpenArray® Human Stem Cell Panel	TaqMan® OpenArray® Pathway Panels
	Characterize		RNAlater® Stabilization Solution	RNAlater® Tissue Collection: RNA Stabilization Solution
	Characterize		RNAqueous® -4PCR Total RNA Isolation Kit	RNAqueous® -4PCR Kit Kit for Isolation of DNA-free RNA
	Characterize		SuperScript® VILO™ Master Mix	Using SuperScript® VILO™ MasterMix
iPSC characterization	Characterize	QuantStudio™ 12K Flex Real-Time PCR System	TaqMan® hPSC Scorecard™ Panel	Using the TaqMan® hPSC Scorecard™ Panel
Detection of transgenes	Characterize	QuantStudio™ 12K Flex Real-Time PCR System	TaqMan® iPSC Sendai Detection Kit	Using the TaqMan® iPSC Sendai Detection Kit
Cell line authentication	Characterize	3130xL Genetic Analyzer	AuthentiFiler™ PCR Amplification Kit	AuthentiFiler™ PCR Amplification Kit
NSC characterization - cytogenetic	Characterize	None	KaryoMAX® Giemsa Stain Solution	Giemsa Banding (“GTG” Banding)
NSC characterization - NSC immunostaining	Characterize	EVOS® FLoid® Cell Imaging Station	Human Neural Stem Cell Immunocytochemistry Kit	Using the Human Neural Stem Cell Immunocytochemistry Kit
NSC characterization - NSC markers flow analysis	Characterize	Attune® Acoustic Focusing Cytometer	antibodies	Attune® Acoustic Focusing Cytometer
Cell line authentication	Characterize	3130xL Genetic Analyzer	AuthentiFiler™ PCR Amplification Kit	AuthentiFiler™ PCR Amplification Kit
Characterization - DA neuron immunostaining	Characterize	EVOS® FLoid® Cell Imaging Station	antibodies	Characterizing NSCs and Differentiated Lineages by Immunocytochemistry
Characterization - glial immunostaining	Characterize	EVOS® FLoid® Cell Imaging Station	antibodies	Characterizing NSCs and Differentiated Lineages by Immunocytochemistry
NSC characterization - gene expression profile	Characterize	QuantStudio™ 12K Flex Real-Time PCR System	TaqMan® OpenArray® Human Stem Cell Panel	TaqMan® OpenArray® Pathway Panels
	Characterize		RNAlater® Stabilization Solution	RNAlater® Tissue Collection: RNA Stabilization Solution
	Characterize		RNAqueous® -4PCR Total RNA Isolation Kit	RNAqueous® -4PCR Kit Kit for Isolation of DNA-free RNA
	Characterize		SuperScript® VILO™ Master Mix	Using SuperScript® VILO™ MasterMix
Gene expression profiling of differentiated NSCs	Characterize	QuantStudio™ 12K Flex Real-Time PCR System	Custom TaqMan® OpenArray® PCR Plate	TaqMan® OpenArray® Pathway Panels
Cell health assays	Characterize		PrestoBlue® Cell Viability Reagent	PrestoBlue® Cell Viability Reagent Protocol
	Characterize		CellEvent™ Caspase-3/7 Green Detection	CellEvent™ Caspase-3/7 Green Detection Reagent
	Characterize		CellROX® Green Reagent	CellROX® Oxidative Stress Reagents
	Characterize		MitoSOX™ Red Mitochondrial Superoxide Indicator	MitoSOX™ Red Mitochondrial Superoxide Indicator
	Characterize		Staurosporine	Using staurosporine to induce apoptosis

Webinars

All webinars were recorded at the 24 Hours of Stem Cells Event, November 2013.

Date	Title
On Demand	Life Technologies and The Parkinson's Institute collaborate to understand the molecular basis for Parkinson's disease Roland Leathers, PhD	View Webinar
On Demand	Generation of induced pluripotent stem cells from Parkinson's disease fibroblasts Connie Lebakken, PhD	View Webinar
On Demand	Generation and characterization of neural stem cells from Parkinson's disease donor-derived iPSCs Kun Bi, PhD	View Webinar
On Demand	Genome editing of Parkinson's disease iPSCs using GeneArt® Precision TALs technology Spencer Hermanson	View Webinar

Seminars

Video	Presented	Year
Creating Integration-free iPSCs and NSCs to Model Parkinson’s Disease by Dr. Birgitt Schuele View seminar	ISSCR	2013

Video

Presented

Year

Creating Integration-free iPSCs and NSCs to Model Parkinson’s Disease by Dr. Birgitt Schuele

View seminar

ISSCR

2013

Scientific Posters

Poster Title	Presented	Year
Efficient generation of induced pluripotent stem cells (iPSCs) derived from Parkinson's disease (PD) study patient fibroblast lines using CytoTune®-iPS 2.0 Sendai Reprogramming Kit in the Essential® feeder-free media system	ISSCR	2014
Genome Editing of iPSCs from Parkinson's Disease and Multiple System Atrophy Patients	ISSCR	2014
Using cells from patients with multiple systems atrophy or Parkinson's Disease (PARK2, LRRK2, and/or GBA mutations) to build stem cell derived disease model systems	ISSCR	2014
Monitoring PD-Relevant Phenotypes in iPSC-generated Neurons from a Spectrum of Parkinson's Disease Patients for Drug Screening and for Understanding the Biology of PD	SFN Chicago	2014
Efficient Generation and Characterization of Neural Stem Cells Derived from Parkinson’s Disease Patient Cells	SFN	2013
Genome editing of alpha-synuclein in iPSCs from a donor with multiple system atrophy	SFN	2013
Generation of transgene-free iPSCs from Parkinson’s disease patients	ISSCR	2013
Novel neural induction method for efficient generation of neural stem cells derived from Parkinson’s Disease and patient-derived sample iPSC lines	ISSCR	2013

Research Highlights

These highlights are brief PDFs describing how a particular product was used in this research.

Title
CytoTune®-iPS Sendai Reprogramming Kit	View PDF
TaqMan® hPSC Scorecard™ Panel	View PDF