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Overview of protein quantitation reagents
Mass spectrometry can detect very low analyte concentrations in complex mixtures. It can be quantitative with the use of peptide labels or standards that are concomitantly analyzed with the sample and act as a reference point for both relative or absolute analyte quantitation, respectively. We offer three different systems that can be applied to solve a wide variety of biological problems. Relative quantitation strategies include tandem mass tagging (TMT) and stable isotope labeling using amino acids in cell culture (SILAC). For both relative and absolute quantitation, SureQuant assays are designed for multiplexed targeted quantitation for key cellular signaling proteins.
Tandem mass tag (TMT) reagents are robust and efficient tools for multiplex relative protein quantitation of up to 18 samples derived from cells, tissues or biological fluids. All mass tagging reagents within a label reagent set have the same nominal mass (i.e., are isobaric) and chemical structure composed of a reactive group, a spacer arm (mass normalizer), and a mass reporter. The isobaric nature of the TMT reagents enables concurrent identification and quantification of proteins in different samples using tandem mass spectrometry (MS).
Learn more about TMT reagents and kits
The SureQuant assays enable multiplexed targeted quantitation for key cell signaling proteins and contain reagents for multiplexed immunoprecipitation and mass spectrometry sample preparation, heavy peptide quantitation panels, and suitability standards to assess system performance. Together, the SureQuant assay system provides flexibility to use the immunoprecipitation sample prep kits and peptide quantitation modules together or independently based on the end application for robust quantitation.
Learn more about SureQuant assay standards and kits
SILAC metabolic labeling relies on in vivo incorporation of “heavy” 13C- or 15N-labeled amino acids into proteins during translation for multiplexed quantitative comparison of “heavy” and “light” protein. It can be widely applied for comprehensive identification, characterization and quantitation of proteins. We offer a broad portfolio of liquid and powdered SILAC media and SILAC amino acids. These reagents are available as kits or as stand-alone reagents.
Learn more about SILAC metabolic labeling reagents
We offer individual reagents and complete kits for both discovery and targeted quantitative proteomic applications.
Benefits of Mass spectrometry reagents:
- Powerful—isobaric tags and metabolic amino acid labeling offer concurrent mass spectrometry analysis of multiple samples, to help increase sample throughput to enable relative quantitation of 2 to 18 different samples derived from cells, tissues, or biological fluids
- Accurate—SureQuant targeted assays and standards provide improved detection enrichment and detection of target proteins for more precise protein quantitation, including low abundant proteins in complex mixtures
- Broad portfolio—complete offering of kits and reagents to support both targeted and discovery protein quantitation
- Compatible—optimized for use with Thermo Scientific LC-MS or LC-MS/MS platforms with data analysis fully supported by Proteome Discoverer software
Check out this simple guide and see which protein quantitation technology is right for your research:
| Labeling molecule | Sample type | Multiplexing sample capability | Technology/Reagent | ||
|---|---|---|---|---|---|
| Cells | Tissues | Biofluids | |||
| Peptide level (In vitro) | ✓ | ✓ | ✓ | Up to 18-plex | Tandem Mass Tags (TMT) |
| Peptide level (In vitro) | ✓ | ✓ | ✓ | None | SureQuant Targeted Assays |
| Protein level (In vivo, Ex vivo) | ✓ | — | — | Up to 3-plex | Stable Isotope Labeling of Amino Acids (SILAC) |
Explore our technical handbook for an integrated overview of protein sample preparation, quantitation and instrument calibration for mass spectrometry, and more.
View and select from our list of qualifying products, eligible for a discount, including TMT reagents, MS-compatible crosslinkers, MS-grade proteases, calibration solutions, standards, and more.
| Order now | Order now | Order now | Order now | Order now | |
| SILAC | TMT | iodoTMT | aminoxyTMT | Peptides for SRM | |
| Method of labeling | Metabolic | Amine-reactive mass tags | Cysteine-reactive mass tags | Carbonyl-reactive mass tags | Spike-in standard |
| Sample type | Cultured mammalian cells | Cultured mammalian cells; | Cultured mammalian cells; tissue; biofluids | Cultured mammalian cells; tissue; biofluids | Cultured mammalian cells; tissue; biofluids |
| Digestion protocol | In-gel | In solution | In solution | In solution | In solution |
| Quantitation mode | MS1 | MS2 (6-plex) | MS2 | MS2 | MS1 |
| Multiplex options | 2-plex, 3-plex | 2-plex, 6-plex, 10-plex | 6-plex | 6-plex | Samples not combined |
Resources and Support
A quick reference brochure that illustrates the workflow and chemical structures of the TMTpro 16-plex label reagents that enable the same labeling efficiency as TMT reagents with improved quantitative accuracy with larger sample sets.
A brochure that highlights products in key areas of mass spectrometry that can help lead to quality results including sample preparation, protein quantitation, protein characterization, and instrument calibration and quality control.
Review this transforming sample preparation platform that can help improve speed, accuracy, and has high reproducibility. This booklet is an overview of the AccelerOme automated sample preparation platform describing features, software, workflow, and integration.
Distinguish between standard peptide synthesis service offering and those of PEPotec Immuno Peptide Libraries, HeavyPeptide and LightPeptide AQUA standards, and PEPotec SRM Peptide Libraries. Find the option suitable for your application.
For Research Use Only. Not for use in diagnostic procedures.