|
2014-05-22
| Transfer
EMSA gels using the Pierce G2 Fast Blotter. The Pierce G2 Fast Blotter
provides rapid semi-dry transfer of DNA (TBE) gels to nylon
membrane. |
Electrophoresis and
Staining, Western Blotting
| Pierce™
G2 Fast Blotter – Power Stainer Upgrade |
|
2014-05-19
| An
improved method for highly efficient isolation of primary mouse embryonic
fibroblasts. Easily isolate and culture primary mouse embryonic fibroblasts
(MEF). |
Lysis and Extraction
| Pierce™
Mouse Embryonic Fibroblast Isolation Kit |
|
2014-05-02
| Pierce
qIP Assay: a novel, HTS-compatible protein-protein interaction assay system.
A cell-based, quantitative co-immunoprecipitation method for protein
interaction analysis involving a sensitive luciferase assay
strategy. |
Immunoprecipitation,
Luciferase Assays
| Pierce™
Agarose qIP Protein Interaction Kit, Tluc and HA Tags |
|
2014-04-02
| One-hour
procedure to isolate primary cardiomyocytes from neonatal mouse and rat
hearts. Easily isolate and culture highly viable, functional primary
cardiomyocytes. |
Lysis and Extraction
| Pierce™
Primary Cardiomyocyte Isolation Kit |
|
2014-03-24
| Glycoprotein
expression in a human IVT system. Expression of partially N-linked
glycoproteins using the 1-Step Human Coupled in vitro Translation
System. |
Protein Expression
| 1-Step
Human Coupled IVT Kit - DNA |
|
2014-03-24
| Benchmarking
experiments with Zeba Spin Desalting Columns. Protein recovery performance
comparisons of Thermo Scientific Zeba Spin Desalting Columns to other
commercially available desalting resins and columns. |
Dialysis and
Desalting
| Zeba™
Micro Spin Desalting Columns, 7K MWCO, 75 µL |
|
2014-03-03
| Calculate
the number of immobilized proteins per bead of agarose affinity supports. A
consideration of relative sizes and dimensions of agarose resin beads,
antibodies, proteins, chemical modification groups, and affinity
ligands. |
Affinity Purification
| AminoLink™
Plus Coupling Resin |
|
2014-02-19
| Improved,
all-in-one B-PER Reagent for bacterial protein extraction. B-PER Complete
Reagent provides gentle, efficient, and cost effective protein extraction
from E. coli and other bacterial cells. |
Lysis and Extraction
| B-PER™
Complete Bacterial Protein Extraction Reagent |
|
2014-01-28
| An
efficient method for the isolation of highly functional primary neurons.
Easily and reproducibly isolate and culture primary neurons. |
Lysis and Extraction
| Pierce™
Primary Neuron Isolation Kit |
|
2014-01-16
| Expression
of highly active proteins using a cell-free human IVT system. Active
caspase-3 produced by cell-free protein expression with the 1-Step Human
High-Yield In Vitro Translation Kit is more functional than recombinant
caspase-3 expressed in bacteria. |
Protein Expression
| 1-Step
Human High-Yield Mini IVT Kit |
|
2014-01-10
| System
for high-throughput, high-yield in vitro translation. HTP protein expression
using 1-Step Human High-Yield IVT Kits and Pierce 96-well Microdialysis
Plates. |
Protein Expression
| 1-Step
Human High-Yield Mini IVT Kit |
|
2013-11-12
| Luciferase
assays and BioTek Synergy microplate readers. Validation and comparison of
single-step flash and dual-spectral luciferase reporter gene assays using the
Synergy line of microplate readers. |
Luciferase Assays
| Pierce™
Renilla Luciferase Flash Assay Kit |
|
2013-10-22
| Choosing
a vector and purification method for in vitro protein expression. Strategies
for optimizing expression and purification of functional proteins produced
using the Thermo Scientific 1-Step High Yield IVT protein expression
system. |
Protein Expression,
Fusion Tag Methods
| pT7CFE1-NHis
Vector for Mammalian Cell-Free Protein Expression |
|
2013-09-11
| Analysis
of androgen-dependent and -independent regulation of transcriptional
activity. Monitor activity using a sensitive and optimized magnetic ChIP
kit. |
Immunoprecipitation,
Molecular Biology
| Pierce™
Magnetic ChIP Kit |
|
2013-08-29
| Efficient
mammalian membrane protein extraction. Fast and simple enrichment of integral
membrane proteins and membrane-associated proteins. |
Lysis and Extraction
| Mem-PER™
Plus Membrane Protein Extraction Kit |
|
2013-08-19
| Specific
labeling, enrichment and quantitation of S-nitrosylated peptides using
iodoTMT reagents. Measure protein and peptide cysteine modifications using
multiplex quantitative mass spectrometry. |
Mass Spectrometry,
Post-translational Modification
| iodoTMTsixplex™
Label Reagent Set, 1 x 0.2 mg |
|
2013-08-02
| Performance
characterization of Pierce Glutathione Superflow Agarose. Purity, dynamic
binding capacity, reusability and chemical compatibility properties of a
glutathione resin for FPLC purification of GST tagged fusion
proteins. |
Fusion Tag Methods,
Affinity Purification
| Pierce™
Glutathione Superflow Agarose |
|
2013-07-11
| Performance
characterization of HisPur Ni-NTA Superflow Agarose. Purity, dynamic binding
capacity, reusability and chemical compatibility properties of a
nickel-charged IMAC resin for FPLC purification of His-tagged
proteins. |
Fusion Tag Methods,
Affinity Purification
| HisPur™
Ni-NTA Superflow Agarose |
|
2013-06-26
| Improved
Western blot detection using CCD digital imaging. Comparison of cooled-CCD
digital imaging versus X-ray film for sensitivity, dynamic range and signal
linearity with enhanced chemiluminescence (ECL). |
Western Blotting,
Electrophoresis and Staining
| myECL™
Imager |
|
2013-06-24
| Versatile
luciferases: new tools for reporter assays. Microplate luminometers equipped
with reagent dispensers and optical filters provide optimal performance for
luciferase assays with Cypridina, Gaussia, Renilla and firefly luciferase
reporter genes. |
Luciferase Assays
| Pierce™
Gaussia Luciferase Flash Assay Kit |
|
2013-06-05
| Carrier
protein activation and conjugation data for immunogen preparation.
Experiments with KLH variants and other immunogens to validate solubility,
maleimide activation consistency, peptide conjugation efficiency and
immunogenicity. |
Antibody Production
| Imject™
mcKLH (in PBS) |
|
2013-06-04
| A
versatile mass spectrometry sample preparation procedure for complex protein
samples. Prepare high-quality peptide digests with high recovery from
cultured cells. |
Mass Spectrometry,
Sample Preparation
| Pierce™
Mass Spec Sample Prep Kit for Cultured Cells |
|
2013-05-22
| Protocol
for in vitro protein expression using mRNA templates. Use the 1-Step Human
Coupled DNA IVT kit to efficiently translate mRNA templates. |
Protein Expression
| 1-Step
Human Coupled IVT Kit - DNA |
|
2013-05-02
| Performance
characterization of HisPur Cobalt Superflow Agarose. Purity, dynamic binding
capacity, reusability and chemical compatibility properties of a
cobalt-charged IMAC resin. |
Fusion Tag Methods,
Affinity Purification
| HisPur™
Cobalt Superflow Agarose |
|
2013-04-30
| Activation
of the antioxidant response pathway by pesticide chemicals. Simultaneous
measurement of the activation of ARE- and NF-κB-driven luciferase reporters
by parkinsonian neurotoxins in neuronal cells. |
Luciferase Assays
| Pierce™
Gaussia Luciferase Flash Assay Kit |
|
2013-04-18
| Separation
characteristics of dialysis membranes. Molecular weight cut-off (MWCO)
specifications and rates of buffer exchange with Slide-A-Lyzer Dialysis
Devices and Snakeskin Dialysis Tubing. |
Dialysis and
Desalting
| Slide-A-Lyzer™
G2 Dialysis Cassettes, 10K MWCO, 3 mL |
|
2013-03-08
| Monitoring
neuronal differentiation using multiplexed luciferase reporters.
Characterization of early neuronal differentiation markers in precursor cell
lines using multiplexed luciferase reporters and immunofluorescence
imaging. |
Luciferase Assays,
IHC and Cell Imaging
| Pierce™
Cypridina Luciferase Flash Assay Kit |
|
2013-03-07
| Chromatography
cartridges for desalting and affinity purification. Data using the cartridge
format for automated liquid chromatography (LC) systems. |
Affinity
Purification, Dialysis and Desalting
| Zeba™
Micro Spin Desalting Columns, 7K MWCO, 75 µL |
|
2013-01-24
| Simultaneous
dual-emission detection of luciferase reporter assays. Performing
dual-spectral luciferase assays using the BMG Labtech POLARstar Omega plate
reader. |
Luciferase Assays,
Protein Expression
| Pierce™
Cypridina-Firefly Luciferase Dual Assay Kit |
|
2012-12-11
| Quantitative
cancer stem cell phosphoprotein profiling. Using tandem mass labeling and
LC-MS/MS to profile perturbations of an autocrine loop. |
Mass Spectrometry,
Post-translational Modification
| Pierce™
Phosphoprotein Enrichment Chromatography Cartridges, 5mL |
|
2012-11-12
| Phosphoprotein
enrichment from cell and tissue samples. Isolate phosphorylated proteins with
an easy-to-use spin format |
Post-translational
Modification, Affinity Purification
| Pierce™
Phosphoprotein Enrichment Chromatography Cartridges, 5mL |
|
2012-10-11
| Cell-free
protein expression system for generating stable isotope-labeled proteins.
Rapidly express protein standards for quantitative mass
spectrometry. |
Mass Spectrometry
| 1-Step
Heavy Protein IVT Kit |
|
2012-07-18
| Eliminate
endotoxins from protein and antibody samples. A highly specific affinity
resin enables endotoxin removal from various protein sources, sizes and
charges. |
Sample Preparation
| Pierce™
High Capacity Endotoxin Removal Resin |
|
2012-07-09
| Removing
endotoxins using a spin-column format. Fast and effective removal of
endotoxins from protein samples. |
Sample Preparation
| Pierce™
High Capacity Endotoxin Removal Resin |
|
2012-06-13
| Luciferase
assays in hard-to-transfect Jurkat cells. Obtain strong signals in cells with
low transfection efficiency. |
Luciferase Assays
| Pierce™
Gaussia Luciferase Flash Assay Kit |
|
2012-05-18
| Fractionate
subcellular proteins from tissue samples. Obtain proteins from five cellular
components using a tissue-optimized kit. |
Lysis and Extraction
| Subcellular
Protein Fractionation Kit for Tissues |
|
2012-05-10
| Method
to isolate functional synaptosomes. Enrich neuronal synaptic proteins while
maintaining phosphoprotein integrity. |
Lysis and Extraction
| Syn-PER™
Synaptic Protein Extraction Reagent |
|
2012-04-18
| Complete
protein protection from proteases and phosphatases. Protease, phosphatase and
combination inhibitor tablets provide a convenient format to protect proteins
from degradation and dephosphorylation. |
Lysis and Extraction,
Sample Preparation
| Pierce™
Protease Inhibitor Tablets, EDTA-free |
|
2012-03-05
| Optimized
protein extraction reagent for neuronal tissue and primary cells. Obtain high
yields of functional neuronal protein from all neuronal cell
compartments. |
Lysis and Extraction
| N-PER™
Neuronal Protein Extraction Reagent |
|
2012-02-28
| Highly
sensitive multiplex luciferase reporter assays. Monitor changes in NFkB and
CRE promoter activity using bioluminescent reporter assays. |
Luciferase Assays,
Protein Expression
| Pierce™
Cypridina-Firefly Luciferase Dual Assay Kit |
|
2012-02-24
| Using
antibody-binding proteins for antibody purification. Choose a format based on
the specific application and scale. |
Antibody
Purification, Affinity Purification
| Pierce™
Protein A Plus Agarose |
|
2011-10-07
| Improved
immobilization and conjugation of glycoproteins. Efficient aldehyde-hydrazide
covalent coupling using a catalyst and an optimized buffer. |
Immunoprecipitation,
Protein Immobilization
| GlycoLink™
IP Kit |
|
2011-06-01
| Western
blotting using enhanced chemiluminescence. Choose the perfect substrate for
your needs. |
Western Blotting
| Pierce™
ECL Western Blotting Substrate |
|
2011-06-01
| Serine
hydrolase active-site probes for activity-based enzyme profiling. New
fluorophosphonate probes selectively label and enrich active serine
hydrolases. |
Protein Labeling,
Protein Assays
| ActivX™
TAMRA-FP Serine Hydrolase Probe |
|
2011-06-01
| Selective
enrichment of sialylated glycopeptides using TiO2 beads. Affinity
purification of glycoproteins for mass spectrometry based on titanium dioxide
beads (TiO2). |
Mass Spectrometry
| Pierce™
TiO2 Phosphopeptide Enrichment and Clean-up Kit |
|
2011-06-01
| Perform
IP and co-IP experiments with high yield and low background. New Protein A/G
magnetic beads enable manual and automated applications. |
Immunoprecipitation,
Affinity Purification
| Pierce™
Protein A/G Magnetic Beads |
|
2011-06-01
| Optimize
liquid chromatography with a peptide retention time calibration mixture. New
mixture of 15 heavy peptides streamlines assay design for targeted peptide
quantification. |
Chromatography, Mass
Spectrometry
| Pierce™
Peptide Retention Time Calibration Mixture |
|
2011-06-01
| Calibration
solutions for Thermo Scientific Mass Spectrometers. Ready-to-use standards
enable quick instrument calibration. |
Mass Spectrometry
| Pierce™
LTQ ESI Positive Ion Calibration Solution |
|
2011-06-01
| New
dimensions for low-volume sample dialysis. Self-contained devices give you the
solution for high-protein recovery. |
Dialysis and
Desalting, Sample Preparation
| Slide-A-Lyzer™
MINI Dialysis Device, 10K MWCO, 0.1 mL |
|
2011-06-01
| Introducing
a Western blot substrate that is chemiluminescent and chemifluorescent.
Detect your target by film, CCD cameras or laser-based imagers. |
Western Blotting
| Pierce™
ECL Plus Western Blotting Substrate |
|
2011-06-01
| Develop
mid- to high-throughput SRM and MRM assays. New application-specific peptide
libraries for quantitative mass spectrometry workflows. |
Peptides, Mass
Spectrometry
| PEPotec
SRM Custom Peptide Libraries |
|
2011-04-05
| Measure
activation of small GTPases via their specific downstream effectors.
Pull-down kits enrich active GTPases from cell or tissue lysates. |
Protein Assays,
Immunoprecipitation
| Active
Rac1 Pull-Down and Detection Kit |
|
2011-04-05
| GTPase
enrichment using a new active-site probe. Introducing a desthiobiotin
nucleotide probe for studying GTPases. |
Protein Labeling,
Affinity Purification
| Pierce™
GTPase Enrichment Kit with GTP Probe |
|
2011-04-05
| Enhanced
immunostaining means more signal and better sensitivity. Achieve better protein
detection using a fraction of your valuable primary antibody. |
IHC and Cell Imaging
| Pierce™
Immunostain Enhancer |
|
2011-04-05
| Detection
and localization of active GTPases in neuronal cell differentiation. GTPase
pull-down kits enable powerful signal transduction studies. |
IHC and Cell Imaging,
Affinity Purification
| Active
Rac1 Pull-Down and Detection Kit |
