Search Thermo Fisher Scientific
Efficient, accurate gene editing is critical to your scientific insights
Whether using CRISPR, TALEN, or prime editing technology, gene editing confirmation is critical for harnessing the full power of genome engineering and avoiding a large loss of time and money in performing downstream experimentation whose interpretation may be compromised. Applied Biosystems Sanger sequencing and fragment analysis systems and free SeqScreener Gene Edit Confirmation App enable in-depth, highly accurate genomic confirmation and analysis by users of all skill levels, for genomic alterations of all types (deletion, insertion, single-base changes) using a simple sample-to-answer workflow.
.svg)
Highly accurate—single base resolution
Simple workflow—rapid time to results
Flexible—confirm any genomic alteration
Insightful—powerful software with intuitive interface
Webinar: Expanded Frontiers of Gene Editing
In this webinar, Dr. Stephen Jackson, applications senior manager at Thermo Fisher Scientific, discusses the utility of genomic tools in modeling diseases, plants, and animals, as well as the importance of genotypic confirmation of gene edits.
Webinar: Gene editing—the precision tool for tomorrow's treatments
In this webinar series, scientists relate how they harness gene editing technologies to advance their HIV research and rare inherited disease studies including Duchenne muscular dystrophy and MECP2 duplication syndrome.
Augment your phenotypic confirmation with Sanger sequencing & fragment analysis: confidence through genomic confirmation
Unlike phenotypic confirmation, performing genotypic confirmation allows researcher to directly attribute a specific genetic alteration to an experimental outcome, enabling researchers to better understand and confidently interpret their gene editing results across every phase of their workflow. This facilitates the rapid development of more efficient engineering experiments, supports definitive go/no-go decisions on advancing studies relying on engineered cells and/or animal models, and maximizes the safety and efficacy of gene editing-based candidate therapies.
Matching the right genetic analysis tool for the right job
모세관 전기영동을 통한 Sanger 시퀀싱은 일차 형질전환된 배양에서 CRISPR과 TALEN 매개 유전체 교정의 효율성을 파악하는 데 사용 가능합니다. Sanger 시퀀싱은 형질전환된 배양에서 유전체 교정 성공 여부를 확인하고 이차 클론의 편집 이벤트 성공 여부를 스크리닝할 수 있는 효율적인 연구 방법입니다. Applied Biosystems™ Minor Variant Finder Software는 이차 배양에서 분리된 클론의 SNP 변화 빈도를 알아내는 데 사용될 수 있습니다.
Workflow가 단순하고 비용 효과적인 것은 물론, 간편한 데이터 분석이 가능하기 때문에, 모세관 전기영동을 통한 Sanger 시퀀싱은 유전체 교정 workflow의 중요한 부분입니다. 개별 서브클론의 Sanger 시퀀싱은 편집 효율 확인 절차를 수월하게 해주는 것은 물론, 발생하는 염기서열 결실과 삽입 범위를 쉽게 알도록 해줍니다. 또한, Sanger 시퀀싱은 개별 세포의 보조 클론에서 유전체 교정을 식별하고 확인하는 손쉬운 방법입니다.
CRISPR 매개 유전체 교정의 전체 workflow
Efficiency and primary pool screening | Secondary clone screening | Targeted confirmation of cells or animal models | |
Cleavage assay-gel |
| - | - |
Cleavage assay – fragment analysis |
| - | - |
TOPO cloning and Sanger sequencing |
| - | - |
Sanger sequencing |
|
|
|
성공한 편집 특징 분석
시스템에 적합한 분석
Powerful bioinformatic tools at your fingertips—no specialized expertise needed
Determine the range and frequency of gene edits with the free, intuitive SeqScreener Gene Edit Confirmation App.
- Everything you need all in one software app—analyzing results to generating publication figures
- Easy-to-use—enables quick identification of successful gene knock-outs and knock-ins
- Fast and accurate—robust algorithm calculates editing frequencies and grades the editing outcome
- Flexible viewing—plate-based overviews to individual sequence trace results
Click
image to enlarge
White paper: Genetic analysis tools for genome editing workflows
Investigate genomic tools including sequencing, fragment analysis, and other genetic analysis methods for determining the efficiency and accuracy of CRISPR/Cas9 gene edits.
Design, build, deliver, detect and validate—the complete gene editing workflow
Thermo Fisher Scientific offers a complete portfolio of products to design, deliver, and confirm gene edits at the phenotypic and genotypic level so you can confidently engineer your cells—all from one place.
Follow the links to learn more about our product offerings for each workflow step. In many cases, the TrueDesign Genome Editor can be used to design and order reagents for the complete editing experiment.
| Design and build | Deliver | Detect and validate | ||
|---|---|---|---|---|
|
|
|
|
|
|
|
| ||
Resources
Webinars
Literature
Blog posts
Customer education
Style Sheet for Global Design System
For Research Use Only. Not for use in diagnostic procedures.