Efficient, accurate gene editing is critical to your scientific insights

Whether using CRISPR, TALEN, or prime editing technology, gene editing confirmation is critical for harnessing the full power of genome engineering and avoiding a large loss of time and money in performing downstream experimentation whose interpretation may be compromised.  Applied Biosystems Sanger sequencing and fragment analysis systems and free SeqScreener Gene Edit Confirmation App enable in-depth, highly accurate genomic confirmation and analysis by users of all skill levels, for genomic alterations of all types (deletion, insertion, single-base changes) using a simple sample-to-answer workflow.

  Highly accurate—single base resolution
  Simple workflow—rapid time to results
  Flexible—confirm any genomic alteration
  Insightful—powerful software with intuitive interface

Webinar: Expanded Frontiers of Gene Editing

In this webinar, Dr. Stephen Jackson, applications senior manager at Thermo Fisher Scientific, discusses the utility of genomic tools in modeling diseases, plants, and animals, as well as the importance of genotypic confirmation of gene edits.

View webinar

Gene-editing

Webinar: Gene editing—the precision tool for tomorrow's treatments

In this webinar series, scientists relate how they harness gene editing technologies to advance their HIV research and rare inherited disease studies including Duchenne muscular dystrophy and MECP2 duplication syndrome.

View webinar

gene-editing-webinar-image


Augment your phenotypic confirmation with Sanger sequencing & fragment analysis: confidence through genomic confirmation
 

Unlike phenotypic confirmation, performing genotypic confirmation allows researcher to directly attribute a specific genetic alteration to an experimental outcome, enabling researchers to better understand and confidently interpret their gene editing results across every phase of their workflow.  This facilitates the rapid development of more efficient engineering experiments, supports definitive go/no-go decisions on advancing studies relying on engineered cells and/or animal models, and maximizes the safety and efficacy of gene editing-based candidate therapies.


Matching the right genetic analysis tool for the right job
 

모세관 전기영동을 통한 Sanger 시퀀싱은 일차 형질전환된 배양에서 CRISPR과 TALEN 매개 유전체 교정의 효율성을 파악하는 데 사용 가능합니다. Sanger 시퀀싱은 형질전환된 배양에서 유전체 교정 성공 여부를 확인하고 이차 클론의 편집 이벤트 성공 여부를 스크리닝할 수 있는 효율적인 연구 방법입니다. Applied Biosystems™ Minor Variant Finder Software는 이차 배양에서 분리된 클론의 SNP 변화 빈도를 알아내는 데 사용될 수 있습니다.

앱 노트 다운로드

Workflow가 단순하고 비용 효과적인 것은 물론, 간편한 데이터 분석이 가능하기 때문에, 모세관 전기영동을 통한 Sanger 시퀀싱은 유전체 교정 workflow의 중요한 부분입니다. 개별 서브클론의 Sanger 시퀀싱은 편집 효율 확인 절차를 수월하게 해주는 것은 물론, 발생하는 염기서열 결실과 삽입 범위를 쉽게 알도록 해줍니다. 또한, Sanger 시퀀싱은 개별 세포의 보조 클론에서 유전체 교정을 식별하고 확인하는 손쉬운 방법입니다.

CRISPR 매개 유전체 교정의 전체 workflow
 
Efficiency and primary pool screening
Secondary clone screening
Targeted confirmation of cells or animal models
Cleavage assay-gel


(manual)

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Cleavage assay – fragment analysis


(automated)

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TOPO cloning and Sanger sequencing


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Sanger sequencing





단일 세포 클론 확립
run sequence
성공한 편집 특징 분석
run sequence

시스템에 적합한 분석



Powerful bioinformatic tools at your fingertips—no specialized expertise needed 
 


gene-editing-whitepaper

White paper: Genetic analysis tools for genome editing workflows

Investigate genomic tools including sequencing, fragment analysis, and other genetic analysis methods for determining the efficiency and accuracy of CRISPR/Cas9 gene edits.

Download white paper


Design, build, deliver, detect and validate—the complete gene editing workflow
 

Thermo Fisher Scientific offers a complete portfolio of products to design, deliver, and confirm gene edits at the phenotypic and genotypic level so you can confidently engineer your cells—all from one place.

gene-editing-workflow-v2_header-01

Follow the links to learn more about our product offerings for each workflow step. In many cases, the TrueDesign Genome Editor can be used to design and order reagents for the complete editing experiment.

Design and buildDeliverDetect and validate

TrueGuide Synthetic gRNA

TrueCut Cas9 Protein v2

TrueTag Donor DNA Kit, GFP

Neon Transfection System

Attune NxT Flow Cytometer, blue

Design tool

CRISPR gRNAs

CRISPR libraries

Cas9 nucleases

  • Fluorescent or epitope tags
  • SNPs and indels
  • Selection markers
  • STOP codons

Learn more ›

  • Transfection reagents
  • Electroporation instruments
  • Cell culture

Learn more ›

  • Cleavage detection
  • Clonal isolation
  • Confirmation of genotype
  • Confirmation of phenotype

Learn more ›

TALEN mRNA pairs

Style Sheet for Global Design System

For Research Use Only. Not for use in diagnostic procedures.