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Table 1.4 Alexa Fluor active esters and kits for labeling proteins and nucleic acids.

Alexa Fluor DyeFluorescence Color (Abs/Em) *Succinimidyl Ester (NHS Ester),
TFP Ester
or SDP Ester
Kits for Labeling Proteins
Kits for Labeling Nucleic Acids and Oligonucleotides
Protein Labeling KitsAntibody Labeling KitsZenon Antibody Labeling Kits
Alexa Fluor 350Blue (346/442)A10168A10170 (P)A20180 (Ab)Z25000 
(M IgG1)

Z25100 
(M IgG2a)

Z25200 
(M IgG2b)

Z25300 
(R IgG)

Z25400 
(H IgG)		 
Alexa Fluor 405Blue (402/421)A30000

A30100		  Z25013 
(M IgG1)

Z25113 
(M IgG2a)

Z25213 
(M IgG2b)

Z25313
 (R IgG)		 
Alexa Fluor 430Yellow-green (434/539)A10169A10171 (P) Z25001 
(M IgG1)

Z25301 
(R IgG)		 
Alexa Fluor 488Green (495/519)A20000 †

A20100 †

A30052 (SDP)

A30005 (TFP)

A37563 (TFP)

A37570 (TFP)		A10235 (P) ‡

A30006 (Micro) ‡		A10468 (APEX) ‡

A20181 (Ab) ‡		Z25002 
(M IgG1)

Z25090 (TSA)
(M IgG1)

Z25102 
(M IgG2a)

Z25202 
(M IgG2b)

Z25302 
(R IgG)

Z25602 
(G IgG)

Z25402 
(H IgG)		U21650 (ULYSIS)

A21665 (ARES)

F32947 (FT)

F32952 (FT)

A20191 (Oligo)
Alexa Fluor 514Yellow-green (518/540)A30002 †    
Alexa Fluor 532Yellow (531/554)A20001

A20101MP		A10236 (P)A20182 (Ab)Z25003 
(M IgG1)

Z25303 
(R IgG)		U21651 (ULYSIS)
Alexa Fluor 546Orange (556/573)A20002 †

A20102 †		A10237 (P)A20183 (Ab)Z25004 
(M IgG1)

Z25104 
(M IgG2a)

Z25204 
(M IgG2b)

Z25304 
(R IgG)		U21652 (ULYSIS)

A21667 (ARES)
Alexa Fluor 555Red-orange (555/565)A20009

A20109

A37564

A37571		A20174 (P)

A30007 (Micro)		A10470 (APEX)

A20187 (Ab)		Z25005 
(M IgG1)

Z25105 
(M IgG2a)

Z25205 
(M IgG2b)

Z25305 
(R IgG)

Z25605 
(G IgG)

Z25405 
(H IgG)		A21677 (ARES)

F32948 (FT)

F32953 (FT)
Alexa Fluor 568Red-orange (578/603)A20003 †

A20103 †		A10238 (P)A10494 (APEX)

A20184 (Ab)		Z25006 
(M IgG1)

Z25106 
(M IgG2a)

Z25206 
(M IgG2b)

Z25306 
(R IgG)

Z25606 (
G IgG)		U21653 (ULYSIS)
Alexa Fluor 594Red (590/617)A20004 †

A20104 †

A37565 †

A37572 †		A10239 (P)

A30008 (Micro)		A10474 (APEX)

A20185 (Ab)

Z25007 
(M IgG1)

Z25107 
(M IgG2a)

Z25207 
(M IgG2b)

Z25307 
(R IgG)

Z25607 
(G IgG)

Z25407 
(H IgG)

U21654 (ULYSIS)

A21669 (ARES)

F32949 (FT)

F32954 (FT)
Alexa Fluor 610Red (612/628)A30050 (X)    
Alexa Fluor 633 §Far-red (632/647)A20005 †

A20105 †		A20170 (P)   
Alexa Fluor 647 §Far-red (650/668)A20006

A20106

A37566

A37573		A20173 (P)

A30009 (Micro)		A10475 (APEX)

A20186 (Ab)

S30044 (SAIVI)		Z25008 
(M IgG1)

Z25108 
(M IgG2a)

Z25208 
(M IgG2b)

Z25308 
(R IgG)

Z25608 
(G IgG)

Z25408 
(H IgG)		U21660 (ULYSIS)

A21676 (ARES)

F32950 (FT)

F32955 (FT)

A20196 (Oligo)
Alexa Fluor 660 §Near-infrared (663/690)A20007A20171 (P) Z25009 
(M IgG1)		U21656 (ULYSIS)

A21671 (ARES)
Alexa Fluor 680 §Near-infrared (679/702)A20008

A20108

A37567

A37574		A20172 (P)A20188 (Ab)

S30045 (SAIVI)		Z25010 
(M IgG1)

Z25110 
(M IgG2a)

Z25210 
(M IgG2b)

Z25310 
(R IgG)		 
Alexa Fluor 700 §Near-infrared (702/723)A20010

A20110		  Z25011 
(M IgG1)		 
Alexa Fluor 750 §Near-infrared (749/775)A20011

A20111

A37568

A37575		 S30046 (SAIVI)Z25312 
(R IgG)		 
Alexa Fluor 790 §Near-infrared (782/805)A30051

A37569		 A20189 (Ab)  
* Approximate absorption (Abs) and fluorescence emission (Em) maxima for conjugates, in nm. † Mixed isomers. ‡ The APEX Alexa Fluor 488 Antibody Labeling Kits contain the Alexa Fluor 488 carboxylic acid SDP ester, whereas the other Alexa Fluor 488 protein labeling kits contain the amine-reactive Alexa Fluor 488 carboxylic acid TFP ester; the Alexa Fluor 488 kits for labeling nucleic acids and oligonucleotides contain the Alexa Fluor 488 succinimidyl ester. The Alexa Fluor Labeling Kits are described in detail in Kits for Labeling Proteins and Nucleic Acids—Section 1.2. § Human vision is insensitive to light beyond ~650 nm, and therefore it is not possible to view the far-red– and near-infrared–fluorescent dyes by looking through the eyepiece of a conventional fluorescence microscope.

(Ab) = Antibody Labeling Kit.
(APEX) = APEX Antibody Labeling Kit.
(ARES) = ARES DNA Labeling Kit.
(FT) = FISH Tag DNA Kit or FISH Tag RNA Kit.
(G IgG) = Zenon Goat IgG Labeling Kit.
(H IgG) = Zenon Human IgG Labeling Kit.
(M IgG) = Zenon Mouse IgG Labeling Kit.
(Micro) = Alexa Fluor Microscale Protein Labeling Kit.
(Oligo) = Alexa Fluor Oligonucleotide Amine Labeling Kit.
(P) = Easy-to-Use Protein Labeling Kit.
(R IgG) = Zenon Rabbit IgG Labeling Kit.
(SAIVI) = SAIVI Antibody Labeling Kit.
(SDP) = Sulfodichlorophenol ester.
(TFP) = Tetrafluorophenyl ester.
(TSA) = Enhanced with TSA technology.
(ULYSIS) = ULYSIS Nucleic Acid Labeling Kit.
(X) = An aminohexanoyl spacer between the dye and the SE.

 

For Research Use Only. Not for use in diagnostic procedures.