Table 7.9 Molecular Probes avidin, streptavidin, NeutrAvidin and CaptAvidin conjugates.

Label (Abs/Em) *StreptavidinNeutrAvidinAvidinCaptAvidin
Fluorophore Conjugates
Alexa Fluor 350 (346/442)S11249A11236  
Marina Blue (365/460)S11221   
Cascade Blue (400/420) A2663  
Alexa Fluor 405 (402/421)S32351   
Pacific Orange (400/551)S32365   
Cascade Yellow (402/545)S11228   
Pacific Blue (410/455)S11222   
Pacific Green (411/500)S11200   
Alexa Fluor 430 (434/539)S11237   
Fluorescein (494/518)S869A2662A821 
Alexa Fluor 488 (495/519)S11223, S32354 † A21370 
Oregon Green 488 (496/524)S6368A6374  
R-Phycoerythrin (496/578) ‡S866, S21388 §A2660  
Alexa Fluor 610–R-PE (496/628) ‡S20982   
Alexa Fluor 647–R-PE (496/668) ‡S20992   
Alexa Fluor 680–R-PE (496/702) ‡S20985   
Alexa Fluor 750–R-PE (496/775) ‡S32363   
Oregon Green 514 (511/530)S6369   
Alexa Fluor 514 (518/540)S32353   
Alexa Fluor 532 (531/554)S11224   
B-Phycoerythrin (546/575) ‡S32350   
Tetramethylrhodamine (555/580)S870A6373  
Alexa Fluor 546 (556/573)S11225   
Alexa Fluor 555 (555/565)S21381, S32355 †   
Rhodamine B (570/590)S871   
Rhodamine Red-X (570/590)S6366A6378  
Alexa Fluor 568 (578/603)S11226   
Alexa Fluor 594 (590/617)S11227, S32356 †   
Texas Red (595/615)S872A2665A820 
Texas Red-X (595/615)S6370   
Alexa Fluor 633 (632/647)S21375   
Alexa Fluor 635 (633/647)S32364   
Alexa Fluor 647 (650/668)S21374, S32357 †   
Allophycocyanin (650/660)S868, S32362 §   
Alexa Fluor 680–allophycocyanin (650/702)S21002   
Alexa Fluor 700–allophycocyanin (650/723)S21005   
Alexa Fluor 750–allophycocyanin (650/775)S21008   
Alexa Fluor 660 (663/690)S21377   
Alexa Fluor 680 (679/702)S21378, S32358 †   
Alexa Fluor 700 (702/723)S21383   
Alexa Fluor 750 (749/775)S21384   
Alexa Fluor 790 (782/805)S11378   
Qdot Nanocrystal Conjugates
Qdot 525 (405/525)Q10141MP **   
Qdot 565 (405/565)Q10131MP **   
Qdot 585 (405/585)Q10111MP **   
Qdot 605 (405/605)Q10101MP **   
Qdot 625 (405/625)Q22063 **
A10196 **
   
Qdot 655 (405/655)Q10121MP **   
Qdot 705 (405/705)Q10161MP **   
Qdot 800 (405/800)Q10171MP **   
FluoSpheres and TransFluoSpheres Microsphere Conjugates
Europium luminescent FluoSpheres microspheres †† (365/610) F20883, F20884  
Yellow-green–fluorescent FluoSpheres microspheres †† (505/515)F8780F8771, F8774, F8776  
Red-fluorescent FluoSpheres microspheres †† (580/605) F8770, F8775  
TransFluoSpheres microspheres †† (488/605) T8860, T8861  
TransFluoSpheres microspheres †† (488/645)T10711   
Nonfluorescent FluoSpheres microspheres †† F8772, F8777  
Gold Conjugates
NANOGOLDN24918   
Alexa Fluor 488 FluoroNanogold (495/519)A24926   
Alexa Fluor 488 colloidal gold (495/519)A32360 ‡‡, A32361 §§   
Alexa Fluor 594 FluoroNanogold (590/617)A24927   
Enzyme Conjugates
Alkaline phosphataseS921   
β-GalactosidaseS931   
Horseradish peroxidaseS911A2664  
β-LactamaseS31569   
Acrylamide and Agarose Conjugates
AcrylamideS21379   
AgaroseS951  C21386
Unlabeled Avidins
UnlabeledS888A2666A887, A2667C21385
* Approximate absorption (Abs) and fluorescence emission (Em) maxima, in nm, for conjugates. † These Alexa Fluor streptavidin conjugates are supplied in 0.5 mL units of a 2 mg/mL solution. ‡ Multiple absorption peaks. § Premium grade. ** Properties and applications of Qdot nanocrystal conjugates are described in Qdot Nanocrystals—Section 6.6. The Qdot Streptavidin Sampler Kit (Q10151MP) contains six different Qdot streptavidin conjugates. †† FluoSpheres and TransFluoSpheres microspheres are available in different diameters, including 0.04 µm, 0.2 µm and 1.0 µm. ‡‡ 5 nm colloidal gold conjugate. §§ 10 nm colloidal gold conjugate.

For Research Use Only. Not for use in diagnostic procedures.