Table 19.2 Comparison of in vitro and in situ Kd values for various Ca2+ indicators.
Indicator
Kdin vitro (nM) *
Kdin situ (nM) †
Cell/Tissue Type
Calcium Green-1
190
930
HeLa cells
Fluo-3
390
2570
Frog skeletal muscle
Fluo-4
345
1000
HeLa cells
Fura-2
145
371
U373-MG astrocytoma cell
Fura-2
145
350
Rabbit gastric gland
Indo-1
230
844
Rabbit cardiac myocyte
Oregon Green 488 BAPTA-1
170
430
HeLa cells
Rhod-2
570
720
Mouse heart
* Values determined at 22°C in 100 mM KCl, 10 mM MOPS, pH 7.2, 0–10 mM CaEGTA. † Values determined in the cellular environments listed in the adjacent column.
For Research Use Only. Not for use in diagnostic procedures.