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Table 12.2 Spectral characteristics of the MitoTracker probes.

Cat. No.MitoTracker ProbeAbs * (nm)Em * (nm)Oxidation State
M7514MitoTracker Green FM †490516NA
M7510MitoTracker Orange CMTMRos551576Oxidized
M7511MitoTracker Orange CM-H2TMRos551 ‡576 ‡Reduced
M7512MitoTracker Red CMXRos578599Oxidized
M7513MitoTracker Red CM-H2XRos578 ‡599 ‡Reduced
M22425MitoTracker Red FM581644NA
M22426MitoTracker Deep Red FM644665NA
* Absorption (Abs) and fluorescence emission (Em) maxima, determined in methanol; values may vary somewhat in cellular environments. † MitoTracker Green FM is nonfluorescent in aqueous environments. ‡ These reduced MitoTracker probes are not fluorescent until oxidized. NA = Not applicable.

For Research Use Only. Not for use in diagnostic procedures.